Assessment of the risk of contracting Lyme disease in areas with significant human presence

Milutinović, Milica; Radulović, Željko; Tomanović, Snežana

doi:10.1590/s0102-09352008000100018

Cited by 5 publications

(4 citation statements)

References 21 publications

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“…On the other hand, a different study (Tomanović et al, 2010) found that over 40% of I. ricinus ticks were infected with these bacteria, similar to the results of the current study. According to prior studies (Milutinovic et al, 2008;Radulović et al, 2010;Tomanović et al, 2010), the following Borrelia species are found in Serbian ticks: B. afzelii, B. garinii, B. valaisiana, and, most frequently, B. lusitaniae.…”

Section: Discussionmentioning

confidence: 94%

Borellia burgdorferi infection in removed ticks and anti-borrelia antibodies in infested patients admitted to the Pasteur institute, Novi Sad

Simin¹,

Lalošević²,

Mijatović

et al. 2020

VET GLASNIK

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Introduction. The primary objectives of this study were (1) to determine the presence of Borrelia burgdorferi infection in ticks removed from patients for the purpose of singling out sites with increased risk of Lyme borreliosis, and (2) to determine the presence of IgM and/or IgG antibodies against B. burgdorferi sensu lato (s. l.) complex in sera of patients who had ticks removed. Materials and Methods. From 108 ticks removed from patients, all were examined zoologically and a sub-sample of 91 ticks was tested using PCR analysis to determine the presence of DNA indicating B. burgdorferi infection. To detect anti-Borrelia IgM and/ or IgG antibodies in 61 patients bitten by ticks, we used line recombinant immunoblot test. Results and Conclusions. The most common tick identified was Ixodes ricinus. B. burgdorferi s. l. was present in 37 of 91 tested ticks (40.7%). Seroconversion against B. burgdorferi s. l. antigen was detected in 12 of 61 patients (19.7%). Most of the infected ticks were from the province of Vojvodina (11 municipalities), with the city of Novi Sad proving to be the site with the highest number of infected ticks, 6 in total.

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Section: Discussionmentioning

confidence: 94%

Borellia burgdorferi infection in removed ticks and anti-borrelia antibodies in infested patients admitted to the Pasteur institute, Novi Sad

Simin¹,

Lalošević²,

Mijatović

et al. 2020

VET GLASNIK

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“…In central, western, and northern Europe, Ixodes ricinus , also known as the castor bean tick, is the most widespread tick species ( de la Fuente et al, 2017 ; Rosa et al, 2018 ). This species has been frequently reported to infest humans and is ( Milutinovic et al, 2008b ) the main vector for a large variety of TBPs transmitted to both humans and animals. Other tick species, such as the ornate cow tick Dermacentor reticulatus , the red sheep tick Haemaphysalis punctata , and the brown dog tick Rhipicephalus sanguineus s.l., are also widely distributed across Europe where reports of tick bites by these species in human is increasing ( Rubel et al, 2021 ).…”

Section: Introductionmentioning

confidence: 99%

Clinical Aspects and Detection of Emerging Rickettsial Pathogens: A “One Health” Approach Study in Serbia, 2020

Banović

Díaz-Sánchez

Simin³

et al. 2022

Front. Microbiol.

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Ticks carry numerous pathogens that, if transmitted, can cause disease in susceptible humans and animals. The present study describes our approach on how to investigate clinical presentations following tick bites in humans. To this aim, the occurrence of major tick-borne pathogens (TBPs) in human blood samples (n = 85) and the ticks collected (n = 93) from the same individuals were tested using an unbiased high-throughput pathogen detection microfluidic system. The clinical symptoms were characterized in enrolled patients. In patients with suspected TBP infection, serological assays were conducted to test for the presence of antibodies against specific TBPs. A field study based on One Health tenets was further designed to identify components of a potential chain of infection resulting in Rickettsia felis infection in one of the patients. Ticks species infesting humans were identified as Ixodes ricinus, Rhipicephalus sanguineus sensu lato (s.l.), Dermacentor reticulatus, and Haemaphysalis punctata. Five patients developed local skin lesions at the site of the tick bite including erythema migrans, local non-specific reactions, and cutaneous hypersensitivity reaction. Although Borrelia burgdorferi s.l., Babesia microti, Anaplasma phagocytophilum, and Candidatus Cryptoplasma sp. DNAs were detected in tick samples, different Rickettsia species were the most common TBPs identified in the ticks. The presence of TBPs such as Rickettsia helvetica, Rickettsia monacensis, Borrelia lusitaniae, Borrelia burgdorferi, Borrelia afzelii, A. phagocytophilum, and B. microti in ticks was further confirmed by DNA sequencing. Two of the patients with local skin lesions had IgG reactive against spotted fever group rickettsiae, while IgM specific to B. afzelii, Borrelia garinii, and Borrelia spielmanii were detected in the patient with erythema migrans. Although R. felis infection was detected in one human blood sample, none of the components of the potential chain of infection considered in this study tested positive to this pathogen either using direct pathogen detection in domestic dogs or xenodiagnosis in ticks collected from domestic cats. The combination of high-throughput screening of TBPs and One Health approaches might help characterize chains of infection leading to human infection by TBPs, as well as prevalence of emerging rickettsial pathogens in the Balkan region.

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“…Total RNA extracted from individual ticks was used as a template, as described previously (Radulović et al Seasonal dynamics of I. ricinus populations in Serbia with extremes of interest for the current study marked: 1, the beginning of tick activity; 2, spring peak; 3, summer minimum; 4, autumn peak. Graph is based on previous results (Milutinović and Bobić 1997, Milutinović 2000, Milutinović and Radulović 2002, Milutinović et al 2008 2010). In the Þrst step, reverse transcription and ampliÞcation of 913 bp long sequence was performed by using SuperScript III One-Step RT-PCR System with Platinum Taq High Fidelity (Invitrogen, Carlsbard, CA), as well as ZR-GPDH-913-F and ZR-GPDH-913-R primers.…”

Section: Methodsmentioning

confidence: 99%

Seasonal and Spatial Occurrence of Glycerol-3-Phosphate Dehydrogenase Variability inIxodes ricinus(Acari: Ixodidae) Populations

Radulović¹,

Milutinović²,

Tomanović³

et al. 2012

J Med Entomol

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Previous results indicate that glycerol-3-phosphate dehydrogenase variability represents the adaptation of Ixodes ricinus L. (Acari: Ixodidae) to fluctuations of environmental conditions, particularly to temperature. Analysis of crucial polymorphisms in I. ricinus Gpdh gene was done by the restriction method, and three different haplotypes were obtained (GPDH441 1, GPDH441 2, and GPDH441 3), corresponding to GPDH alleles detected by allozyme electrophoresis. Differences in GPDH441 haplotype and genotype frequencies were found between samples from open and forest habitats. Significant seasonal variations of GPDH441 haplotype and genotype frequencies were detected in samples from the open habitats. No seasonal variations were observed at forest localities, probably because of the less pronounced amplitude of environmental factors. The possible role of host availability was discussed as an important factor that affects seasonal dynamics and genetic composition of tick populations.

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Assessment of the risk of contracting Lyme disease in areas with significant human presence

Cited by 5 publications

References 21 publications

Borellia burgdorferi infection in removed ticks and anti-borrelia antibodies in infested patients admitted to the Pasteur institute, Novi Sad

Borellia burgdorferi infection in removed ticks and anti-borrelia antibodies in infested patients admitted to the Pasteur institute, Novi Sad

Clinical Aspects and Detection of Emerging Rickettsial Pathogens: A “One Health” Approach Study in Serbia, 2020

Seasonal and Spatial Occurrence of Glycerol-3-Phosphate Dehydrogenase Variability inIxodes ricinus(Acari: Ixodidae) Populations

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