Assessment of the role of flavonoids for inducing osteoblast differentiation in isolated mouse bone marrow derived mesenchymal stem cells

Srivastava, Swati; Bankar, Rohini; Roy, Partha

doi:10.1016/j.phymed.2013.03.001

Cited by 81 publications

(58 citation statements)

References 35 publications

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“…It was shown that quercetin at low concentrations stimulate the proliferation of human cells and was suggested for the treatment of certain diseases, in contrast to the higher concentrations that induce apoptosis and was suggested as potential anticancer drug [18]. Similar results were described by Srivastava et al [7], who observed that this flavonoid increase the proliferation of mouse bone quantified (a-c, e). The results represent the mean of three independent experiments performed in triplicate ± SEM.…”

Section: Discussionsupporting

confidence: 76%

“…Metabolic specificities of each cell type interfere directly with the effect of flavonoids [7]. It was shown that quercetin at low concentrations stimulate the proliferation of human cells and was suggested for the treatment of certain diseases, in contrast to the higher concentrations that induce apoptosis and was suggested as potential anticancer drug [18].…”

Section: Discussionmentioning

confidence: 99%

“…The nature of mesenchymal cells, together with their multipotentiality, may render these cells an excellent choice for many strategies and cell-based therapies [7]. Similarly, stromal cells exhibit MSCs characteristics, such as immunosuppressive properties, transdifferentiation potential and cell phenotype, thus, they are functionally comparable to MSCs [5,8].…”

Section: Introductionmentioning

confidence: 99%

“…This discrepancy in the effects of flavonoids may be attributed to the fact that the positive and negative actions of these phytochemicals are also dependent on cell types and metabolic disorders involved [7]. Due to conflicting results with regard to the adverse properties of flavonoids, it is important to evaluate its safety [17,24].…”

Section: Introductionmentioning

confidence: 99%

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Quercetin and Rutin Affect the Survival and Proliferation of Human Skin-Derived Multipotent Mesenchymal Stromal Cells

Almeida¹,

Trentin²,

Nones³

2015

JPP

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Abstract:Flavonoids are phenolic compounds with biological and pharmacological properties, such as antioxidant and antiviral effects. In the present work, we evaluated the effect of the flavonoids quercetin and rutin in human SD-MSCs (skin-derived-multipotent mesenchymal stromal cells). Cultured SD-MSCs were exposed to different concentrations of flavonoids (80 to 320 µM) for 2 days in vitro. Cell viability was assessed by MTT assay and cell proliferation by BrdU staining. Cell death was quantified by the analysis of picnotic nuclei. In this paper, we demonstrated for the first time that both quercetin and rutin affect the viability of SD-MSCs, although high concentrations of quercetin (320 µM) promoted increased values of picnoctic nuclei. Quercetin treatment increased cell proliferation and, in contrast, rutin in the same concentration decreased these values. Our results may aid the comprehension of flavonoids effect in SD-MSCs. However, a better understanding of the mechanisms involving flavonoids and SD-MSCs interactions are necessary.

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Section: Discussionsupporting

confidence: 76%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Quercetin and Rutin Affect the Survival and Proliferation of Human Skin-Derived Multipotent Mesenchymal Stromal Cells

Almeida¹,

Trentin²,

Nones³

2015

JPP

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“…Phytophenols which can suppress ROS-induced oxidative stress, therefore, may suggest a possible new approach to maintain MSC viability and potency for clinical application. 1) Because of this, several types of phytophenols have been investigated for the beneficial effects on MSCs, such as flavonoids (quercetin and rutin), 3) lignan (honokiol), 4) coumarin (7,8-dihydroxy coumarin), 5) anthraquinone (emodin), 6) phenolic acid (protocatechuic acid), 7) stilbene (resveratrol), 8) epigallocatechin-3-gallate (EGCG), and curcumin. 1) Our recent studies have demonstrated that a phenolic acid ester (chlorogenic acid) and a phenolic ketone (maclurin) could also effectively protect MSCs from damage induced by hydroxyl radical (·OH), which is the most harmful form of ROS.…”

Section: Protective Effect Of Sinapine Against Hydroxyl Radical-inducmentioning

confidence: 99%

Protective Effect of Sinapine against Hydroxyl Radical-Induced Damage to Mesenchymal Stem Cells and Possible Mechanisms

Han

et al. 2016

CHEMICAL & PHARMACEUTICAL BULLETIN

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As a phenolic alkaloid occurring in Cruciferous plants, sinapine was observed to protect mesenchymal stem cells (MSCs) against ·OH-induced damage in this study. It was also found to prevent DNA from damage, to scavenge various free radicals (·OH, ·O 2 , 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid diammonium salt) (ABTS) · , and 1,1-diphenyl-2-picrylhydrazyl radical (DPPH·)), and to reduce Cu 2 to Cu . To further explore the mechanism, the end-product of sinapine reaction with DPPH· was determined using HPLC-electrospray ionization ( ·OH-induced oxidative damage is radical-scavenging, which is thought to be via hydrogen atom (H·) transfer (HAT) (or sequential electron (e) proton transfer (SEPT))→RAF pathways.Key words sinapine; mesenchymal stem cell; antioxidant mechanism; Cruciferous plant; ·OH-induced damage; hydrogen atom (H·) transfer (HAT) (or sequential electron (e) proton transfer (SEPT)) radical adduct formation (RAF) Recently, some phytophenols have been reported to suppress reactive oxygen species (ROS)-induced oxidative stress in mesenchymal stem cells (MSCs).1) MSCs are known as multipotent fibroblast cells that give rise to cells of the skeletal connective tissue including osteoblasts, chondrocytes, and adipocytes. However, their clinical applications are limited in their expandability and differentiation capacity, because oxidative stress from ROS impact a number of cellular processes, including cell adhesion, migration, and proliferation.2) Phytophenols refer to the phenolic compounds occurring in medicinal plants (especially Chinese herbal medicines). Phytophenols which can suppress ROS-induced oxidative stress, therefore, may suggest a possible new approach to maintain MSC viability and potency for clinical application.

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Biocomposite Scaffolds Derived from Renewable Resources for Bone Tissue Repair

Thangaraj

Selvamurugan

2017

Handbook of Composites From Renewable Materials

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Assessment of the role of flavonoids for inducing osteoblast differentiation in isolated mouse bone marrow derived mesenchymal stem cells

Cited by 81 publications

References 35 publications

Quercetin and Rutin Affect the Survival and Proliferation of Human Skin-Derived Multipotent Mesenchymal Stromal Cells

Quercetin and Rutin Affect the Survival and Proliferation of Human Skin-Derived Multipotent Mesenchymal Stromal Cells

Protective Effect of Sinapine against Hydroxyl Radical-Induced Damage to Mesenchymal Stem Cells and Possible Mechanisms

Biocomposite Scaffolds Derived from Renewable Resources for Bone Tissue Repair

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