Assessment of the sevenband grouper Epinephelus septemfasciatus with a live nervous necrosis virus (NNV) vaccine at natural seawater temperature

Oh, Myung‐Joo; Gye, Hyun Jung; Nishizawa, Tsutomu

doi:10.1016/j.vaccine.2013.02.049

Cited by 34 publications

(8 citation statements)

References 24 publications

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“…It should be noted that the infection with sub-optimal doses of live virus by immersion induced an effect, measured by the presence of significant antibody levels after 30 days (Figure 1) [15, 20], and this could raise the possibility of vaccinating fish with a live/attenuated virus, as already shown in a grouper species ( E. septemfasciatus ) [52, 53]. However, the use of live pathogens for fish immunization raises concerns about their containment and poses risks of vertical/horizontal transmission [27].…”

Section: Discussionmentioning

confidence: 92%

A formalin-inactivated immunogen against viral encephalopathy and retinopathy (VER) disease in European sea bass (Dicentrarchus labrax): immunological and protection effects

Nuñez-Ortiz

Pascoli

Picchietti

et al. 2016

Vet Res

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The European sea bass (Dicentrarchus labrax) is an important farmed fish species in the Mediterranean area, very sensitive to the infection by encephalopathy and retinopathy virus (VERv), or Betanodavirus, which causes massive mortalities. Effective vaccines to fight the pathology are not yet available and in this work we describe a promising intraperitoneal immunization route against VERv of sea bass juveniles. We performed intraperitoneal and immersion immunization trials with a VERv (isolate 283.2009 RGNNV) inactivated by formalin, β-propiolactone and heat treatment. Interestingly, the intraperitoneal immunization with formalin-inactivated VERv induced a significant antigen-specific IgM production, differently from other inactivation protocols. However, the same formalin-inactivated antigen resulted in very low IgM antibodies when administered by immersion. Following the intraperitoneal injection with formalin-inactivated virus, the quantitative expression of the antiviral MxA gene showed a modulation of transcripts in the gut after 48 h and on head kidney after 24 h, whereas ISG12 gene was significantly up-regulated after 48 h on both tissues. In immersion immunization with formalin-inactivated VERv, a modulation of MxA and ISG12 genes after 24 h post-treatment was detected in the gills. An effective uptake of VERv particles in the gills was confirmed by immunohistochemistry using anti-VERv antibodies. Lastly, in challenge experiments using live VERv after intraperitoneal immunization with formalin-inactivated VERv, we observed a significant increase (81.9%) in relative survival percentage with respect to non-immunized fish, whereas immersion immunization resulted in no protection. Our results suggest that intraperitoneal immunization with formalin-inactivated VERv could be a safe and effective strategy to fight Betanodavirus infection in European sea bass.Electronic supplementary materialThe online version of this article (doi:10.1186/s13567-016-0376-3) contains supplementary material, which is available to authorized users.

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Section: Discussionmentioning

confidence: 92%

A formalin-inactivated immunogen against viral encephalopathy and retinopathy (VER) disease in European sea bass (Dicentrarchus labrax): immunological and protection effects

Nuñez-Ortiz

Pascoli

Picchietti

et al. 2016

Vet Res

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“…Extracted RNA (A) (Kasai et al, 2000). VNN Nishizawa et al, 2012;Oh et al, 2012;2013a;2013b;Tanaka et al, 2001;Yamashita et al, 2009). 예방용 백신투여 .…”

Section: Nnvmentioning

confidence: 99%

“…, (Nishizawa et al, 2012;Oh et al, 2013a;Tanaka et al, 2001;Yamashita et al, 2009). NNV , VNN [RPS (relative percentage survival): < 40.8%] 10 7 TCID 50 /fish (Yamashita et al, 2009).…”

Section: Nnvmentioning

confidence: 99%

Prevention Strategies for Viral Nervous Necrosis (VNN) in Sevenband Grouper Epinephelus septemfasciatus Aquaculture Farms

Kim¹,

Kim²

2015

Korean Journal of Fisheries and Aquatic Sciences

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Viral nervous necrosis (VNN) is a serious disease of sevenband grouper Epinephelus septemfasciatus in Korean aquaculture farms. However, we suggest the following preventative methods for hatcheries: 1) disinfecting rearing water, 2) selecting spawners via ELISA and PCR, 3) selecting eggs via PCR, 4) disinfecting fertilized eggs, and 5) proper facilities management. When these methods are implemented, nervous necrosis virus (NNV)-free fish are produced because vertical and horizontal transmission is prevented. However, horizontal transmission of NNV through rearing seawater sourced from the environment during grow-out stages in sea cages can still occur. Live NNV vaccines with a low rearing temperature or Poly(I:C) immunization are very effective at preventing horizontal transmission of NNV in rearing farms. Furthermore, even after VNN is contracted, fish mortality can be reduced by administering Poly(I:C).

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“…Huge losses occur worldwide in the grouper aquaculture industry every year due to NNV infection [2,9]. Although various vaccines have been developed, such as formalin-in activated and virus-like particle vaccines [10][11][12][13], it might be relatively difficult to inject the vaccines into grouper larvae and juveniles [14], which are the primary targets of RGNNV infections. Another approach to reduce RGNNV is interferon (IFN) introduction to fish, which was reported to prevent viral infection in vivo and in vitro [3,[15][16][17][18].…”

Section: Introductionmentioning

confidence: 99%

Secretory Production of Functional Grouper Type I Interferon from Epinephelus septemfasciatus in Escherichia coli and Bacillus subtilis

Lin

Joe

et al. 2020

IJMS

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Nervous necrosis virus (NNV) results in high mortality rates of infected marine fish worldwide. Interferons (IFNs) are cytokines in vertebrates that suppress viral replication and regulate immune responses. Heterologous overexpression of fish IFN in bacteria could be problematic because of protein solubility and loss of function due to protein misfolding. In this study, a protein model of the IFN-α of Epinephelus septemfasciatus was built based on comparative modeling. In addition, PelB and SacB signal peptides were fused to the N-terminus of E. septemfasciatus IFN-α for overexpression of soluble, secreted IFN in Escherichia coli (E-IFN) and Bacillus subtilis (B-IFN). Cytotoxicity tests indicated that neither recombinant grouper IFN-α were cytotoxic to a grouper head kidney cell line (GK). The GK cells stimulated with E-IFN and B-IFN exhibited elevated expression of antiviral Mx genes when compared with the control group. The NNV challenge experiments demonstrated that GK cells pretreated or co-treated with E-IFN and B-IFN individually had three times the cell survival rates of untreated cells, indicating the cytoprotective ability of our recombinant IFNs. These data provide a protocol for the production of soluble, secreted, and functional grouper IFN of high purity, which may be applied to aquaculture fisheries for antiviral infection.

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Assessment of the sevenband grouper Epinephelus septemfasciatus with a live nervous necrosis virus (NNV) vaccine at natural seawater temperature

Cited by 34 publications

References 24 publications

A formalin-inactivated immunogen against viral encephalopathy and retinopathy (VER) disease in European sea bass (Dicentrarchus labrax): immunological and protection effects

A formalin-inactivated immunogen against viral encephalopathy and retinopathy (VER) disease in European sea bass (Dicentrarchus labrax): immunological and protection effects

Prevention Strategies for Viral Nervous Necrosis (VNN) in Sevenband Grouper Epinephelus septemfasciatus Aquaculture Farms

Secretory Production of Functional Grouper Type I Interferon from Epinephelus septemfasciatus in Escherichia coli and Bacillus subtilis

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