Assessment of the taxonomic status of the members of genus Artocarpus (Moraceae) in Sri Lanka

Senavirathna, H. M. T. N.; Ranaweera, L. T.; Mudannayake, M. M. A. W. P.; Nawanjana, P. W. I.; Wijesundara, W. M. D. A.; Jayarathne, H. S. M.; Ratnasuriya, M. A. P.; Weebadde, Cholani

doi:10.1007/s10722-020-00902-x

Cited by 5 publications

(1 citation statement)

References 30 publications

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“…The PCR was carried out in 50 µL of total reaction mixture comprised of 25 µL of Go Taq® Green Master Mix, 2× Green Go Taq® Reaction Buffer (pH 8.5), 400 µL dATP, 400 µL dGTP, 400 µL dCTP, 400 µL dTTP, 3 mM MgCl 2 , 1 µL of forward primer, 1 µL of reverse primer, template DNA, and nuclease free water. The PCR cyclic process conducted in the Thermal Cycler (Takara, Otsu Shiga, Japan) was consisted of initial denaturation at 94 °C for 5 mins, followed by 35 cycles including 30 sec of denaturation at 94 °C, 1 min annealing at 55 o C and 2 mins extension at 72 °C followed by final extension of 10 mins at 72 °C (Senavirathna et al, 2020). The PCR products were visualized using 1 % agarose gel electrophoresis and purified using QIAquick PCR purification kit (Qiagen, Hilden, Germany).…”

Section: Pcr and Dna Sequencingmentioning

confidence: 99%

<em>Potentila indica (Andr.) nov.</em> (wild strawberry) in Sri Lanka is Restricted to a small Climatic Envelop Urging Strict Conservation

et al. 2021

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Purpose: The nomenclature of the wild strawberries inhabited in Sri Lanka is ambiguous. In Sri Lanka, this species is still named Duchesnea indica which needs a revision. Wild strawberries grow well in natural habitats of upcountry in Sri Lanka. Since the commercial strawberry cultivations gain a popularity in upcountry, the studies on wild strawberry is essential for crop improvement and management.Research Method: In the present study, we conducted extensive field sampling followed by a phylogenetic analysis with the DNA barcoding markers ITS and, trnL-F by using a representative sample of wild strawberry plants in Sri Lanka. The distribution of the species was identified using maximum entropy modeling approaches.Findings: Sri Lankan wild strawberry got placed at subtribe: Potentilla, and clade: Reptans and show a shallow divergence with the species Potentilla indica reported. Thus, we reposition the genus of wild strawberries in Sri Lanka from Duchesnea to Potentilla and hereafter name it as P. indica. The niche model analysis predicted a highly restricted distribution of Sri Lankan wild strawberry in Nuwara-Eliya district over an area of 166.36 km2 in the altitude range of 1546 - 2524 m in a small climatic envelop highlighting the need for urgent conservation measures.Research Limitations: The pop-set for available in literature of P. indica is limited for comparison. Extensive studies based on DNA sequencing is needed for further validation.Originality / Value: Taxonomy, narrow distribution, need of conservation, and phylogenetic distance to Fragaria chiloensis, a progenitor species of cultivated strawberry, are defined for Sri Lankan wild strawberries.

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Section: Pcr and Dna Sequencingmentioning

confidence: 99%

<em>Potentila indica (Andr.) nov.</em> (wild strawberry) in Sri Lanka is Restricted to a small Climatic Envelop Urging Strict Conservation

et al. 2021

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Artocarpus heterophyllus (jackfruit)

Bakewell-Stone¹

2023

CABI Compendium

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Assessing diversity and phylogeny of Indonesian breadfruit (Artocarpus spp.) using internal transcribed spacer (ITS) region and leaf morphology

Mursyidin

Setiawan

2023

Journal of Genetic Engineering and Biotechnology

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Background Breadfruit (Artocarpus spp.) is the main genus of Moraceae with multipurpose benefits, both ecologically and economically important, e.g., food ingredients, building materials, traditional medicine, and natural insecticides. However, most endemic Artocarpus have been threatened due to natural disasters and habitat degradation. The objective of our study was to determine the genetic diversity and relationships of endemic Artocarpus from South Borneo, Indonesia, using an internal transcribed spacer (ITS) region and leaf morphology. Results Morphologically, endemic Artocarpus endemic to South Borneo, Indonesia, has a different leaf shape, i.e., narrow-obovate to broad-elliptic, from simple to deeply dissected. Following the ITS region, this germplasm has a moderate level of nucleotide diversity (0.069). The phylogenetic analysis revealed Artocarpus into four (4) main clades, where the nearest is shown by the ‘Puyian’ (Artocarpus rigidus) and ‘Binturung’ (Artocarpus odoratissimus) at a coefficient divergence of 0.027, whereas the furthest by ‘Kulur’ (A. camansi) and ‘Tiwadak’ (A. integer) at a coefficient of 0.132. Conclusion In brief, although an endemic Artocarpus of South Borneo, Indonesia, has a moderate level of nucleotide diversity, this germplasm also shows a unique phylogenetic relationship. Thus, this information is urgent in supporting the future Artocarpus breeding and preservation programs, mainly to save this germplasm from being threatened.

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Assessment of the taxonomic status of the members of genus Artocarpus (Moraceae) in Sri Lanka

Cited by 5 publications

References 30 publications

<em>Potentila indica (Andr.) nov.</em> (wild strawberry) in Sri Lanka is Restricted to a small Climatic Envelop Urging Strict Conservation

<em>Potentila indica (Andr.) nov.</em> (wild strawberry) in Sri Lanka is Restricted to a small Climatic Envelop Urging Strict Conservation

Artocarpus heterophyllus (jackfruit)

Assessing diversity and phylogeny of Indonesian breadfruit (Artocarpus spp.) using internal transcribed spacer (ITS) region and leaf morphology

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