Assessment of the value of immunohistochemistry in the subtyping of acute leukemia on routinely processed bone marrow biopsy specimens with particular reference to macrophage-associated antibodies

The detection of CD14 and CD16 in paraffin-embedded bone marrow biopsies is useful for the diagnosis of chronic myelomonocytic leukemia

“…This might confirm what has been suggested concerning the lower sensitivity of CD163 and CD68/PG-M1 antibodies for leukemic monocytic cells compared to tissue histiocytes/macrophages [9,14,15]. Moreover, PG-M1 has not been found as a good marker in discriminating between the subtypes of acute myeloid leukemia M4/5 and M1/2 [16]. CD14 is known to be expressed by monocytes, interstitial myeloid DC, and various types of tissular macrophages [10,17,18].…”

The sonic hedgehog factor GLI1 imparts drug resistance through inducible glucuronidation

“…The Infinity 1-2C-184976 camera was used to capture images and images were analyzed on the Infinity Analyze image acquisition software (release 5.0.2, 2002–2009 Infinity corporation). Lysozyme staining was as described previously 23 . Images for lysozyme staining were captured Leica DM LB2 microscope, and Leica DFC 350X camera and displayed with Adobe Photoshop 7.0 software.…”

Paraffin Section Immunophenotyping of Acute Leukemias in Bone Marrow Specimens

“…6 Antibodies directed against monocytes were fairly nonspecific when used to detect myeloid lineage, 6 " 8 and the ability to detect immature B-cell neoplasms was limited. 3 ' 5 In the last few years, however, major advances have occurred in the methodology of paraffin section immunohistochemistry.…”