Assessment of tissue-specific effect of cadmium on antioxidant defense system and lipid peroxidation in freshwater murrel, Channa punctatus

Abstract

The effects of different concentrations of cadmium chloride on the extent of lipid peroxidation (LPO) and alterations in the antioxidant enzyme activities were studied in liver, kidney and gill tissues of freshwater murrel, Channa punctatus. The fish specimens were exposed to 6.7, 13.4 and 20.1 mg l(-1) sublethal concentrations of cadmium chloride and the oxidative stress was assessed after 24, 48, 72 and 96 h post-exposure. The biomarkers selected for the study were thiobarbituric acid reactive substances for assessing the extent of lipid peroxidation and antioxidant defense system such as reduced glutathione (GSH), glutathione reductase (GR), glutathione peroxidase (GP(X)), glutathione-S-transferase (GST), catalase (CAT) and superoxide dismutase (SOD) activities. In general, the cadmium exposure elevated the LPO in subject tissues of treated group and modulated the activities of GPx, GST, SOD, CAT, GR and level of GSH after given exposure as compared to the control. All enzymes activities, except CAT (in kidney and gills), and amount of LPO elevated significantly (P < 0.05) in treated group with respect to control in all tissues, while significant difference was not observed between the exposed concentrations and within exposure duration. The results indicated that increase in LPO level and the fluctuation in antioxidant defense system in fish could be due to cadmium-induced increase in the production of reactive oxygen species (ROS). The potential role of these parameters as biomarkers of heavy metal pollution in aquatic system is discussed.

Studies on combined effect of Aeromonas hydrophila and cadmium on lipid peroxidation and antioxidant status in selected tissues of Indian freshwater major carp, Catla catla: role of silver nanoparticles

“…On the other hand, LPx levels were significantly enhanced in these tissues of fishes exposed to Cd alone or in combination with AH infection. The results are in consonance with previous reports (26)(27)(28). It is well known that Cd severely affects structural and functional integrity of tissues through oxidative stress.…”

Effect of dietary carob (Ceratonia siliqua) syrup on blood parameters, gene expression responses and ammonia resistance in tilapia (Oreochromis niloticus)

“…Some studies reported that additives in fish feed decreased the amounts of GOT and GPT (Ji et al, ; Kesbic, Acar, Yilmaz, & Aydin, ; Rao, Das, Jyotyrmayee, & Chakrabarti, ; Shalaby, Khattab, & Abdel Rahman, ). GPx, which is a protective antioxidant, acts along with CAT to deactivate the negative effects of ROS by scavenging hydrogen peroxide and other peroxides (Dabas et al, ). In our study, the amount of GPx did not change.…”

Molecular cloning and expression patterns of copper/zinc superoxide dismutase and manganese superoxide dismutase in Musca domestica

“…In addition, Cd can compete with essential metals for protein-binding sites (Pruski and Dixon, 2002), inducing the release of Fe 2+ and Cu 2+ ions and increasing the production of ROS (Xu et al, 2011). In general, the Cd 2+ exposure elevated the lipid peroxidation in subject tissues and modulated the activities of SOD or other antioxidant defense systems as well (Dabas et al, 2011). In our research, the expression level of SOD which was particularly higher in the experimental group than in the control increased with the Cd 2+ concentration ranging from 0.2 to 5 mM in the medium.…”