Assessment of total phenolic content and antioxidant potentiality of selected Indian folk medicinal plants by spectrophotometric method

Abstract

<jats:p>Natural antioxidant capacity of five important folk medicinal plants measured in vitro. Total phenolic content (TPC), flavonoid content (FC) and free radical scavenging capacity of ethanolic, methanolic and aqueous leaf extracts of Lippia alba (LA), Annona squamosa (AS), Hyptis suaveolens (HS), Commiphora wightii (CW) and Milletia pinnata (MP) was assessed using spectrophotometric method. Folin ciocalteu and aluminium chloride method employed to optimise TPC and FC. Free radical scavenging potentiality of leaf extracts was assessed using Ferrous ion chelation (FIC), 2, 2'-diphenyl-1-picrylhydrazyl (DPPH•) scavenging, Hydroxyl (OH•) radical scavenging (HRS) and Superoxide (O- 2) radical scavenging (SRS) methods. Results revealed that the TPC (96.22±5.85 to 519.23±34.90 μg GAE/gm dry weight) were found significant in aqueous extracts from all the plants except AS (p&lt;0.05). For FC (μg QCE/gm dry weight), ethanol was found optimum for LA (463.94±6.49), CW (289.99±2.70) and MP (347.47±4.50) whereas, aqueous was found more appropriate for rest two plants were found significant instead of ethanol and methanol (all p&lt;0.05). The lowest IC50 (μg/ml) were recorded from A. squamosa (27.72±8.95), H. suaveolens (27.78±0.88), C. wightii (27.18±0.16) and M. pinnata (27.30±0.03). All plants have reflected a high antioxidant capacity; however, the highest antioxidant activity was reported from ethanolic extract of H. suaveolens followed by L. alba, A. squamosa, C. wightii and M. pinnata. Hence, these studies show that all folk medicinal plants contain potential antioxidant bioactive compounds.</jats:p>

Antibacterial Activity and Anxiolytic Effect in Adult Zebrafish of Genus Lippia L. Species

“…In terms of total flavonoids, LgEE had the highest rate of these compounds, with 8.76 ± 0.27 mg QE/g Ext., followed by LsEE and LaEE (Table 2). However, these contents were lower than those obtained for different extracts of L. sidoides, with 43.5 ± 0.3 to 262.3 ± 0.4 mg QE/g extract, and of L. alba, with 371.33 ± 4.50 to 463.94 ± 6.49 g QE/gm dry weight [53,54]. Many studies consider the influence of genetic factors, abiotic factors, seasonality, and type of extraction on the composition of a species [55].…”

“…The results show that LaEE has a significantly higher content of total phenolics of 30.11 ± 1.24 mg GA/g Ext., followed by LgEE and LsEE (Table 2). Studies show a high phenolic content for ethanolic, methanolic, and aqueous extracts of L. alba leaves, with 117.78 ± 2.69, 367.49 ± 38.90, and 505.11 ± 2.55 µg GAE/g dry weight, respectively [53]. Garmus et al [54], studying the influence of different types of extraction on the phenolic composition of L. siodides, obtained contents ranging between 38.20 ± 0.06 and 230.5 ± 0.1 mg GAE/g extract.…”

Phytochemical analysis, antioxidant and anti-inflammatory activity of leaves and bark of Ceropegia rollae Hemadri

“…These free radicals are the main factor for the lipid peroxidation. Ceropegia rollae has certain bioactive compounds, which have good antioxidant and anti-inflammation activities (8)(9)(10).…”