Assessment of Total Phenolic Content and Antioxidant Activity Potential of Clementine Extract Obtained by Microwave Assisted Extraction Method

Abstract

Citrus peels are known as rich source of bioactive compounds. Therefore, the establishment of an appropriate and innovative extraction method is a major interest to the food processing industry. The aim of this work is to assess the total phenolic content (TPC) and antioxidant activity (AA) of peels extracts prepared from five Moroccan clementine varieties. Extracts were prepared with microwave-assisted extraction (MAE) technique using acetone and methanol solvents. The evaluation of the antioxidant activity was performed by DPPH and ABTS- free radical scavenging. The extraction conditions had significant effects on total phenolic content (TPC) and antioxidant activity (AA). In fact, the highest TPC amount of clementine varieties (730.850±0.004mg GAE/100g DM) was obtained with acetone solvent for the Sidi Aissa variety. Regarding antioxidant activity, results with DPPH method showed an important antiradical and antioxidant activities for Sidi Aissa variety with methanol solvent (46.30± 1.01%) . However, ABTS method showed a good antioxidant activity for Ain Taoujdat with methanol solvent (3.02 mg/100g DM). This work provided to study the biochemical quality of Moroccan clementine extracts obtained by two microwave-assisted extraction processes in order to investigate further industrial potential uses in agro-food and pharmaceutical industries

Optimizing Microwave-Assisted Extraction from Levisticum officinale WDJ Koch Roots Using Pareto Optimal Solutions

“…Therefore, the design and optimization of extraction processes is becoming increasingly important. This is because it is of particular importance not only in preventing the loss of bioactive compounds, which are sensitive to process parameters, but also in minimizing the cost intensity of the extraction procedure itself [37]. In our research, the process variables that determined the efficiency of bioactive compound extraction were extraction time, microwave power, and the ratio of plant biomass to the solvent.…”

Physiological and biochemical responses of hybrid maize (Zea mays L.) varieties grown under heat stress conditions

“…Flag leaves of five randomly selected plants were sampled from each plot between the tasseling and silking phenological periods. A 100 mg sample of each of the flag leaf samples was wrapped in aluminum foil and quickly transferred to the laboratory within a nitrogen tank at −198 °C, and then placed in a refrigerator at −20 °C ( Azzouzi et al, 2021 ). A 100 g sample of plant tissues from each sample was then homogenized in 3 mL methanol (80%), and then more 80% methanol was added until the final volume of the homogenate reached 5 mL.…”