Assessment of Total Phenolic, Flavonoid, Tannin Content and Phytochemical Screening of Leaf and Flower Extracts from Peltophorum pterocarpum (DC.) Backer ex K.Heyne: a comparative study.

Abstract

Introduction: Total phenolic, flavonoid and tannin content of leaf and flower extract of Peltophorum pterocarpum (DC.) Backer ex K.Heyne was compared. Objective: To explore total phenolic, flavonoid and tannin content of both leaf and flower extracts of Peltophorum pterocarpum(DC) K Heyne. Method: Initially, collected fresh leaves and flower samples were shade dried and extracted with various solvents such as aqueous methanol (1:1), ethyl acetate, ethanol and aqueous. Qualitative analysis was performed for various phytochemical. Thenthe total phenolic content, total flavonoid content and total tannin content was estimated. Results: In preliminary phyto-chemical examination of various solvent extracts of both leaf and flowers of P. pterocarpum revealed that the presence of various phytochemicals such as phlobatannins, terpenoids, alkaloids, saponins, tannin, reducing sugars, phenols and steroids. In phtyochemical evaluation, when compare with all other solvents, Ethanolic extracts shows maximum extractive value. In case of ethyl acetate, it shows very low extractive value in all three phyto-chemicals. In phytochemical evaluation studies, total phenolic content of leaves shows highest in ethanolic extract (33.17 ± 4.72 mg/g) and lowest in ethyl acetate extract from flower (4.71 ± 0.07 mg/g), Similarly, flavonoid content of leaves shows highest in ethanolic extract

Investigation of Antibacterial and Anti-Diarrhoeal Activities of 80% Methanol Leaf and Fruit Extract of Leonotis ocymifolia (Burm. F) Iwarsson (Lamiaceae)

“… 35 In addition to these, phytochemical constituents like tannins and saponins are also endowed with anti-diarrheal activity. 36 …”

Enhanced bioavailability and anti‐hyperglycemic activity of young apple polyphenols by complexation with whey protein isolates

“…The recovery of phenolic compounds in WPI‐YAP complexes was determined by diluting the prepared YAP and WPI‐YAP solutions to 50 ml with distilled water, and then centrifuging at 15,000 × g for 15 min. The 0.5 ml supernatant was transferred to a centrifuge tube containing 1 ml 100% methanol, followed by mixing fully and centrifuging at 15,000 × g for 15 min, after which the supernatant was collected, and the content of phenolic compounds and total polyphenols was quantified by high performance liquid chromatography (HPLC) and Folin‐phenol method, respectively (Li et al., 2019; Muthukumaran et al., 2016). To assess the bioaccessibility of YAP with and without WPI added, 8 ml of the freshly prepared YAP, WPI‐YAP solutions were subject to a three‐stage in vitro digestion including oral, stomach and small intestine (Gayoso et al., 2016) (Table 1).…”

Determination of Total Flavonoid Levels of Ethanol Extract Sesewanua Leaf (Clerodendrum Fragrans Wild) With Maceration Method Using UV-Vis Spectrofotometry

“…This leaves are washed washed thoroughly with running water to remove the dirt from these samples. 20 These samples were blotted dry with tissue papers and carried out chopped with a uniform size, then carried out drying in the oven at 60°C for 3 days after drying dry sorting is done to separate the damaged parts and then mashed using a blender to produce simplicia 500 grams of sesewanuwa leaf powder. 12 The simplisia extraction process of sesewanua leaves (Clerodendrum fragrans Willd) with the maseration method Weigh as much as 500 g of simplicia leaves of an animal and then add 96% ethanol 21 3.5 L until the simplicia is completely submerged.…”