Assessment of total phenolic, total flavonoid, metal contents and antioxidant activities of Trametes versicolor and Laetiporus sulphureus

Abstract

Antioxidant activities, metal contents, total phenolics and flavonoids were analyzed for Trametes versicolor and Laetiporus sulphureus collected from Kastamonu region by using ICP-OES and spectrophotometric methods. Antioxidant activity of mushroom extracts were expressed as percentage of DPPH radicals inhibition and IC50 values. Percentage of inhibition ranged from 15.83 to 61.03% and IC50 value was found 5.33 mg/mL for L. sulphureus and % inhibition ranged from 7.27 to 20.47% and IC50 value was found 18.10 mg/mL for T. versicolor. The content of phenols in methanolic extracts expressed in gallic acid equivalents varied between 77.41±1.10 and 272,70±2.26 mg/g for T. versicolor and L. sulphureus respectively. Also the content of flavonoids in methanolic extracts expressed in quercetin equivalents varied between 13.82±0.21 and 44.29±0.54 mg/mg for T. versicolor and L. sulphureus, respectively. As a result the significant linear correlation was corroborated between the values for the total phenolic content and antioxidant activity of this mushroom. In addition, all samples were analyzed by ICP-OES to obtain the concentration of Co, Cu, Cd, Pb, Ni, Cr, Na, Ca, Al, Fe, Zn, Ba, P, Mg, As, Mn and B. While maximum and minimum metal contents of mushrooms were found as mg/kg for Na (1.88-2.09), Mg (4.59-6.77), P (17.11-24.52), Ca (0.49-23.91) and Fe (0.49-3.84), the maximum and minimum trace metal contents of mushrooms were found as µg/kg for Cr (8.23-35.27), Ni (41.51-180.99), Cu (36.36-123.93).

The Possibility of Using Sulphur Shelf Fungus (Laetiporus sulphureus) in the Food Industry and in Medicine—A Review

“…Strong antibacterial and antifungal, and even antioxidant effects were associated with substances present in the polysaccharide extract [ 21 ]. The authors of the study suggest that extracts from the fruiting bodies of L. sulphureus may be a potential natural food preservative [ 21 , 40 ], which is also confirmed by the results of other studies [ 8 , 79 ].…”

“…The antioxidant activity of L. sulphureus extracts is similar to that of extracts obtained from the fruiting bodies of the fungus Pleurotus ostreatus [ 1 ], but three times higher than in the case of Trametes versicolor (free radical-scavenging capacity of the extract determined by the DPPH method; [ 79 ]). In another study, Laetiporus sulphureus was characterized by the lowest antioxidant activity (determined by the FRAP method) among five fungal species tested (3.5 mmol Trolox/kg dw).…”

“…Antioxidant properties of L. sulphureus . Due to their rich and diverse chemical composition, extracts obtained from L. sulphureus show antioxidant activity, which is most often assessed as high [ 79 , 80 , 85 , 88 ], especially when samples are obtained from dried mushrooms, not mycelium [ 84 ]. It is higher than the antioxidant activity of rosemary and coffee acids [ 80 ].…”

“…The content of polyphenols in water extracts was higher than in ethanol extracts (43.9 and 9.8 mg GAE/100 g DW, respectively) [ 90 ]. Bulam et al [ 79 ] reported that a L. sulphureus extract contained more than three times more phenolic compounds and flavonoids than a Trametes versicolor extract (272.7 mg GAE/g and 44.3 mg QE/mg, respectively, in L. sulphureus , and 77.4 mg GAE/ g and 13.8 QE/mg, respectively, in T. versicolor ). In the study of Nicolcioiu and colleagues [ 89 ], the total phenolic content in L. sulphureus was higher only than the amount found in Hericium coralloides (111.7 mg GAE/l), while the highest value was found in Agaricus campestris (489.2 mg GAE/l).…”

Assessment of Metal Elements and Biochemical Constituents of Wild Turkey Tail (Trametes versicolor) Mushrooms Collected from the Shivalik Foothills of the Himalayas, India

“…Therefore, the metal element bioaccumulation order in collected mushrooms was as follows: Fe > Zn > Mn > Cu > Cr > Ni > Co > Cd. By contrast, Bulam et al [29] mentioned a metal element accumulation in wild T. versicolor in the following order: Ni > Cu > Cr > Cd > Co. This difference could be attributed to the difference in soil composition among the sampling sites.…”