Assessment of Total Polyphenol Compounds in the Complex Phytopreparations Angionorm and Prostanorm Using the Folin-Ciocalteu Method

Стручков, Петр Андреевич; Beloborodov, V. L.; Ilyasov, Igor R.; Savvatee, A. M.; Kolkhir, V. K.; Voskoboinikova, I. V.

doi:10.1007/s11094-016-1474-2

Cited by 2 publications

(2 citation statements)

References 13 publications

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“…because of the lack of specificity and high reactivity of the reagents employed in the FCR assay, this assay actually detects a wide array of electron donating species, e.g. [11] [13] and accordingly, a wide range of organic solvents cannot be used to extract polyphenols due to a possible interaction between the solvent itself and the FCR. Also, when a more efficient extracting procedure than the aqueous one is used, an extraction of a considerable amount of organic reducing species from the soil is likely to occur and the presence of any such oxidizable species in the extract may contribute to the color formation and thus to a false positive outcome in the FCR procedure.…”

Section: Obstacles Encountered In the Determination By The Fcr Assay ...mentioning

confidence: 99%

Critical Examination of the Suitability of the Folin-Ciocalteu Reagent Assay for Quantitative Analysis of Polyphenols—The Case of Olive-Mill Wastewater

Samara¹,

Ahmed²,

Mingelgrin³

2022

AJAC

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The suitability of the Folin-Ciocalteu reagent (FCR) and of HPLC for analyzing polyphenols is evaluated. FCR assay is commonly used although its flows, such as overestimating polyphenol content due to interference by oxidizable species, were previously reported. The wide range of oxidizable species present in biological systems seriously compromises this assay's reliability. Adding small amounts of protein to olive-mill wastewater (OMWW) significantly increased the apparent polyphenol content indicated by the FCR assay. The commonly used "reference" polyphenol as a standard for "total polyphenols" quantification is problematic since each polyphenol responds differently to the FCR. Conversely, HPLC may underestimate polyphenol content. No single HPLC protocol is likely to detect the whole myriad of polyphenols which may be present in a polyphenol-containing system. In analyzing 5 OMWW samples both by FCR assay and HPLC, the polyphenol content indicated by the FCR assay was up to six-fold higher than that determined by HPLC.

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Section: Obstacles Encountered In the Determination By The Fcr Assay ...mentioning

confidence: 99%

Critical Examination of the Suitability of the Folin-Ciocalteu Reagent Assay for Quantitative Analysis of Polyphenols—The Case of Olive-Mill Wastewater

Samara¹,

Ahmed²,

Mingelgrin³

2022

AJAC

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“…The obtained TEAC values agree acceptably with the TEAC values of 0.278 AE 0.004 mmol g À1 reported for the aqueous-alcoholic extract of a mixture of medicinal herb substances including licorice root and Echinacea rhizomes. 36…”

Section: Evaluation Of the Teac Of Echinacea Extractmentioning

confidence: 99%

Potentiometric and UV-Vis spectrophotometric titrations for evaluation of the antioxidant capacity of chicoric acid

Cheng

Zhang

et al. 2020

RSC Adv.

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Assessment of Total Polyphenol Compounds in the Complex Phytopreparations Angionorm and Prostanorm Using the Folin-Ciocalteu Method

Cited by 2 publications

References 13 publications

Critical Examination of the Suitability of the Folin-Ciocalteu Reagent Assay for Quantitative Analysis of Polyphenols—The Case of Olive-Mill Wastewater

Critical Examination of the Suitability of the Folin-Ciocalteu Reagent Assay for Quantitative Analysis of Polyphenols—The Case of Olive-Mill Wastewater

Potentiometric and UV-Vis spectrophotometric titrations for evaluation of the antioxidant capacity of chicoric acid

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