Assessment of toxicity and differential antimicrobial activity of methanol extract of rhizome of Simaba ferruginea A. St.-Hil. and its isolate canthin-6-one

Gazoni, Vanessa Fátima; Balogun, Sikiru Olaitan; Arunachalam, Karuppusamy; Oliveira, Darley Maria; Filho, Valdir Cechinel; Lima, Samara Rosolem; Colodel, Edson Moleta; Soares, Ilsamar Mendes; Ascêncio, Sérgio Donizeti; Martins, Domingos Tabajara de Oliveira

doi:10.1016/j.jep.2018.05.014

Cited by 30 publications

(9 citation statements)

References 44 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…However, the structure–activity relationship (SAR) for the antifungal activity remained unclear. A high antimicrobial activity of canthin-6-one against Staphylococcus aureus and Saccharomyces cerevisiae has also been reported [ 37 ].…”

Section: Resultsmentioning

confidence: 99%

In Vitro Antimicrobial and Antiproliferative Activities of the Root Bark Extract and Isolated Chemical Constituents of Zanthoxylum paracanthum Kokwaro (Rutaceae)

Kaigongi

Lukhoba

Yaouba

et al. 2020

Plants

View full text Add to dashboard Cite

Zanthoxylum paracanthum Kokwaro (Rutaceae) is an endemic Kenyan and Tanzanian plant used in folk medicine by local populations. Although other Zanthoxylum species have been studied, only Z. paracantum stem extracts have been profiled, even though the roots are also used as herbal remedies. As root extracts may be another source of pharmaceutical compounds, the CH2Cl2/MeOH (1:1) root bark extract was studied in this report. Eight root bark compounds were isolated and their structural identities were confirmed by mass spectrometry (MS) and nuclear magnetic resonance (NMR) (using COSY, HSQC, NOESY and HMBC) analyses. The structural identities were determined as follows: the fatty acid—myristic acid (1); the sterol—stigmasterol (2); the lignan—sesamin (3); two β-carboline alkaloids—10-methoxycanthin-6-one (6) and canthin-6-one (7); and three phenanthridine alkaloids—8-acetonyldihydrochelerythrine (4), arnottianamide (5) and 8-oxochelerythrine (8). Some of these compounds were identified in the species for the first time. These compounds and the extract were then tested in vitro against methicillin-resistant Staphylococcus aureus (MRSA), Escherichia coli (ATCC 25922), Staphylococcus aureus (ATCC 29213) and Candida albicans (ATCC 10231) before tests for antiproliferative activity against the human breast cancer (HCC 1395), human prostate cancer (DU 145) and normal (Vero E6) cell lines were conducted. Minimum inhibition concentration values of 3.91, 1.95, 0.98 and 7.81 µg/mL against MRSA, S. aureus, E. coli and C. albicans, respectively, were recorded. Among the isolates, canthin-6-one was the most active, followed by 10-methoxycanthin-6-one. The root extract and some of the compounds also had antiproliferative activity against the HCC 1395 cell line. Stigmasterol and canthin-6-one had IC50 values of 7.2 and 0.42. The root bark extract also showed activity, at 8.12 µg/mL, against the HCC 1395 cells. Out of the chemical isolates, 10-methoxycanthin-6-one and canthin-6-one showed the strongest inhibition of the DU 145 cells. The root extract had significant antimicrobial and antiproliferative activities, supporting the traditional use of this plant in treating microbial infections and cancer-related ailments.

show abstract

Section: Resultsmentioning

confidence: 99%

In Vitro Antimicrobial and Antiproliferative Activities of the Root Bark Extract and Isolated Chemical Constituents of Zanthoxylum paracanthum Kokwaro (Rutaceae)

Kaigongi

Lukhoba

Yaouba

et al. 2020

Plants

View full text Add to dashboard Cite

show abstract

“…The hot neem extract contained previously known antioxidants that includes methyl gallate (Lubis et al 2018), vinyl sulphide (Ianiski et al 2018), 5, hydroxymethylfurfural (Zhao et al 2013), 6-hydroxyl-3-phenyl coumarin (Matos et al 2015), cyclic octa atomic sulfur (Ojinnaka et al 2015). The anti-HIV metabolites include methyl gallate (0.39%), canthin-6-one (2.02%) from neem hot and simarouba cold acetone extracts, respectively (Guvensen et al 2019;Wang et al 2014;Ahmed et al 2017), while 2,4-di-tert-butylphenol and vanillic acid are also anticancer agents besides its antimicrobial, antioxidants activity (Gazoni et al 2018;Yemis et al 2011;Dehpour et al 2012). On the other hand, angelic acid is identified as the potent antioxidants, spasmolytic, besides its usefulness to treat nervous problems (Varsha et al 2015).…”

Section: Discussionmentioning

confidence: 99%

The antimicrobial and antioxidant property, GC–MS analysis of non-edible oil-seed cakes of neem, madhuca, and simarouba

Gowda

Kumarswammy

et al. 2021

Bull Natl Res Cent

View full text Add to dashboard Cite

Background The antimicrobial activity against clinically and agriculturally important microorganisms, antioxidant property and chemical profiling of acetone crude extracts of non-edible oil-seed cakes of neem (Azadirachta indica A. Juss), madhuca (Madhuca longifolia syn. Madhuca indica Gmelin) and simarouba (Simarouba glauca DC) obtained by hot and cold extraction methods were tested in-vitro. Results The hot neem and cold acetone extracts of madhuca and simarouba were inhibitory to Staphylococcus aureus. The enteric pathogens-Enterococcus faecalis and Salmonella enterica were inhibited by both hot and cold extracts of simarouba cake. Fusarium oxysporum and Colletotrichum capsici were sensitive to oil-seed cake extracts of madhuca and simarouba cake extracts followed by neem cake extract. The Aspergillus flavus was highly sensitive to neem followed by madhuca and simarouba extracts. The cyclic voltammetry of all extracts indicated the presence of oxidation peaks at different Epa values suggesting the presence of antioxidant ability. The GC–MS profile revealed the presence of pharmacologically important essential compounds. Conclusion The study revealed the presence of bioactive compounds in non-edible oil-seed cakes that could be exploited for human welfare.

show abstract

“…A nucleic acid leakage assay was carried out to detect the integrity of the bacterial cell membrane (Gazoni et al, 2018). S. mutans in the exponential phase was diluted to 2 × 10 8 CFU/ ml in 0.9% NaCl and treated with GHc or GHd at 0.5 × MIC, 1 × MIC, or 2 × MIC at 37°C for 0, 30, 60, 90, 120, and 150 min.…”

Section: Nucleic Acid Leakage Via the Bacterial Membranementioning

confidence: 99%

Antibacterial and Antibiofilm Activity of Temporin-GHc and Temporin-GHd Against Cariogenic Bacteria, Streptococcus mutans

et al. 2019

View full text Add to dashboard Cite

Temporin-GHc (GHc) and temporin-GHd (GHd) produced by the frog Hylarana guentheri had shown broad-spectrum antibacterial activities against bacteria and fungi. In this study, we investigated whether they exert antibacterial and antibiofilm activities against cariogenic bacteria, Streptococcus mutans. GHc and GHd adopt the random coil conformation in aqueous solution and convert to α-helix in membrane mimetic environments by using circular dichroism spectroscope. They are positively charged by histidine, with the polar and nonpolar amino acids on opposing faces along the helix. The amphipathicity enabled the peptides to target at bacterial membrane, leading to an increase in membrane permeation and disruption of S. mutans, which allowed the peptides to bind with genomic DNA. GHc and GHd completely impeded the initial attachment of biofilm formation and disrupted preformed S. mutans biofilms. The expression of exopolysaccharide (EPS) biosynthesis genes which synthesize glucosyltransferases in S. mutans was downregulated by exposing to GHc or GHd, contributing to the decrease of soluble and insoluble EPS. GHc and GHd exhibited selectivity toward S. mutans in the presence of human erythrocytes, and no cytotoxicity toward human oral epithelial cells was observed at a concentration of 200 μM. These results laid the foundation for the development of GHc and GHd as potential anti-caries agents.

show abstract

Assessment of toxicity and differential antimicrobial activity of methanol extract of rhizome of Simaba ferruginea A. St.-Hil. and its isolate canthin-6-one

Cited by 30 publications

References 44 publications

In Vitro Antimicrobial and Antiproliferative Activities of the Root Bark Extract and Isolated Chemical Constituents of Zanthoxylum paracanthum Kokwaro (Rutaceae)

In Vitro Antimicrobial and Antiproliferative Activities of the Root Bark Extract and Isolated Chemical Constituents of Zanthoxylum paracanthum Kokwaro (Rutaceae)

The antimicrobial and antioxidant property, GC–MS analysis of non-edible oil-seed cakes of neem, madhuca, and simarouba

Antibacterial and Antibiofilm Activity of Temporin-GHc and Temporin-GHd Against Cariogenic Bacteria, Streptococcus mutans

Contact Info

Product

Resources

About