Assessment of toxicity and genotoxicity of low doses of 5-fluorouracil in zebrafish ( Danio rerio ) two-generation study

Kovács, Róbert; Csenki, Zsolt; Bakos, Katalin; Urbányi, Béla; Horváth, Ákos; Garaj‐Vrhovac, Vera; Gajski, Goran; Gerić, Marko; Negreira, Noelia; Alda, Miren López de; Barceló, Damià; Heath, Ester; Kosjek, Tina; Žegura, Bojana; Novak, Matjaž; Zajc, Irena; Baebler, Špela; Rotter, Ana; Ramšak, Živa; Filipič, Metka

doi:10.1016/j.watres.2015.03.025

Cited by 93 publications

(37 citation statements)

References 48 publications

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“…Zebrafish are amenable small teleost, incessantly used for drug screening, efficacy studies and toxicity testing [65,68,69,86] . Although cardiotoxicity, behavior alteration or teratogenecity determinations can be determined using zebrafish, to evaluate hepatotoxicity derived from new generation drugs treatments is still under testing.…”

Section: Resultsmentioning

confidence: 99%

“…Adult zebrafish were exposure to a 2-week treatment of the alkylating agent methyl methanesulfonate. The comet assay was employed to evaluate the presence of micronuclei in gonad, liver, or gild [68,69] . On the basis of this study, zebrafish can be considered an efficient vertebrate model to study genotoxicity through comet assay and the micronucleus test.…”

Section: An Animal Model To Evaluate Genotoxicitymentioning

confidence: 99%

“…As a note, this cell line compared to another fish cell line (PLHC-1, trout hepatocytes), showed less sensitivity upon short-term exposure to the genotoxicants tested [61][62][63] . Thus, those reports bring to light zebrafish as in vivo and in vitro model to study genotoxicity [68][69][70][71][72] .…”

Section: An Animal Model To Evaluate Genotoxicitymentioning

confidence: 99%

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Zebrafish as Toxicological model for screening and recapitulate human diseases

Caballero¹,

Candiracci²

2018

JUMD

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Embryonic and larval Danio rerio (zebrafish) is increasingly used as a toxicological model to conduct rapid in vivo tests and developmental toxicity assays; the zebrafish features high genetic homology to mammals, robust, phenotypes, highthroughput genetic and chemical screening have made it a powerful tool to evaluate in vivo toxicity. New methodologies of genome editing as CRISPR/Cas9; ZFN and TALEN make it a suitable model to perform studies to pair human genetic diseases as well. This review surveys recent studies employing zebrafish as experimental model, comparing it with other in vivo and in vitro models, presenting zebrafish as a potent vertebrate tool to evaluate drug toxicity and efficacy in order to facilitate more extensive, easy and comprehensive knowledge of new generation drugs.

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Section: Resultsmentioning

confidence: 99%

Section: An Animal Model To Evaluate Genotoxicitymentioning

confidence: 99%

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Zebrafish as Toxicological model for screening and recapitulate human diseases

Caballero¹,

Candiracci²

2018

JUMD

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“…Despite lack of clarity about the possible damage of these compounds to the environment, it is necessary to establish the concentration value for a safe exposure due to the way these kinds of compound act. They could have genotoxic effects and, in addition, a mixture of them could be more dangerous that the single compounds [32][33][34].…”

Section: Analysed Compoundsmentioning

confidence: 99%

Applications of Fabric Phase Sorptive Extraction to the Determination of Micropollutants in Liquid Samples

et al. 2018

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Abstract:The occurrence of organic micropollutants (OMPs) in the environment is a global concern due to their potential ecological risks. Several studies have shown that some OMPs are widely detected in environmental matrices such as surface water and sewage. Wastewater treatment plants (WWTPs) have received international attention over past decades because they are considered the greatest source of aquatic environmental contamination by anthropogenic micropollutants. Intensive sampling and analysis have been globally made to improve understanding of the occurrence, behavior and fate of OMPs in WWTPs using different types of analytical approach. Recently, special awareness has been devoted to developing new effective strategies to extract the micropollutants of wastewater. In particular, microextraction protocols have gained popularity because of their simplicity, low cost and in-field application for environmental analysis. Among these, fabric phase sorptive extraction (FPSE) is reported as an excellent approach due to its properties, not only reducing the required time but also employing minor solvent volume. In this overview, we summarize the results obtained by the Research Group of Environmental Chemical Analysis of the University of Las Palmas de Gran Canaria (Spain) using this technique. Its aim is to show the potential of FPSE for the extraction of some micropollutants, such as personal care products (benzotriazole ultraviolet stabilizers (BUVSs)) and pharmaceuticals (steroid hormones and cytostatic compounds) in different liquid samples, prior to their determination by liquid chromatography.

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“…The tested drugs were characterized by low acute and sub-chronic toxicity, which indicates low susceptibility of fish towards these drugs. However, a previously published study by Kovacs et al (2015) reports how chronic two-generation exposure of zebrafish to 5-FU at environmentally relevant concentrations (10 ng L −1 ) caused histopathological changes in the liver and kidney, impaired their DNA integrity and induced massive whole-transcriptome changes. It can be concluded that standard acute and sub-acute toxicity tests, recommended by EMA guidelines (EMA 2006), are not adequate for predicting of potential delayed adverse effects of anticancer drugs.…”

mentioning

confidence: 98%