Assessment of tuber coxae bone biopsy in the standing horse

Mitchell, Colin F.; Richbourg, Heather A.; Goupil, Brad A.; Gillett, Ashley N.; McNulty, Margaret A.

doi:10.1111/vsu.12603

Cited by 3 publications

(7 citation statements)

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“…Horses were restrained in stocks and sedated with IV xylazine (Xylamed, Bimeda, Cambridge, ON, Canada) (0.35 to 0.5 mg/kg). The surgical method has been previously published in detail [ 17 ]. In short, the biopsy site was centered on the proximal palpable protuberance of the tuber coxae.…”

Section: Methodsmentioning

confidence: 99%

“…Lidocaine (Lidocaine 2%, VetOne, Boise, ID, USA) was injected subcutaneously 4 cm proximal and distal to the palpable tuber coxae, and then deeply to the periosteum of the tuber coxae. The horse was further sedated with detomidine (Dormosedan, Zoetis, Kalamazoo, MI, USA) (3 to 5 mg IV) and butorphanol tartrate (Torbugesic, Fort Dodge, New York, NY, USA) (3 to 5 mg IV) prior to making the skin incision [ 17 ].…”

Section: Methodsmentioning

confidence: 99%

“…The surgery site was lavaged, and then the subcutaneous tissue and skin were sutured separately. An aluminium based bandage spray (Aluspray, Neogen Corporation, Lexington, KY, USA) was applied to the surgery site and a lidocaine patch (Lidocaine Patch 5%, Qualitest, Huntsville, AL, USA) was then applied over the incision [ 17 ].…”

Section: Methodsmentioning

confidence: 99%

“…The gold standard for bone analyses are histomorphometry [ 15 ] and micro-computed tomography (microCT) [ 16 ], both of which require bone biopsies. The tuber coxae has been reported to be the easiest site for bone biopsy acquisition in the horse [ 17 , 18 ] and is a region that was found to have consistently higher levels of tiludronate in comparison to other bones [ 19 ]. A study published by the authors has shown the tuber coxae as a site to obtain reliable bony samples in a non-terminal equine model that are consistent in size and of adequate quality to evaluate trabecular bone using both histomorphometry and microCT [ 17 ].…”

Section: Introductionmentioning

confidence: 99%

“…Additionally, the effect of clodronate on bone healing after an induced defect was also investigated. These evaluations were performed by obtaining bone biopsies of the tuber coxae using an established technique [ 17 ], and evaluated through histomorphometry and microCT. To evaluate bone healing, subsequent biopsies were taken from the initial biopsy site, which served as a novel bone defect model in the horse.…”

Section: Introductionmentioning

confidence: 99%

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Tiludronate and clodronate do not affect bone structure or remodeling kinetics over a 60 day randomized trial

et al. 2018

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BackgroundTiludronate and clodronate are FDA-approved bisphosphonate drug therapies for navicular disease in horses. Although clinical studies have determined their ability to reduce lameness associated with skeletal disorders in horses, data regarding the effect on bone structure and remodeling is lacking. Additionally, due to off-label use of these drugs in young performance horses, effects on bone in young horses need to be investigated. Therefore, the purpose of this randomized, experimental pilot study was to determine the effect of tiludronate and clodronate on normal bone cells, structure and remodeling after 60 days in clinically normal, young horses. Additionally, the effect of clodronate on bone healing 60 days after an induced defect was investigated.ResultsAll horses tolerated surgery well, with no post-surgery lameness and all acquired biopsies being adequate for analyses. Overall, tiludronate and clodronate did not significantly alter any bone structure or remodeling parameters, as evaluated by microCT and dynamic histomorphometry. Tiludronate did not extensively impact bone formation or resorption parameters as evaluated by static histomorphometry. Similarly, clodronate did not affect bone formation or resorption after 60 days. Sixty days post-defect, healing was minimally affected by clodronate.ConclusionsTiludronate and clodronate do not appear to significantly impact bone tissue on a structural or cellular level using standard dose and administration schedules.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Tiludronate and clodronate do not affect bone structure or remodeling kinetics over a 60 day randomized trial

et al. 2018

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Clinically relevant doses of tiludronate do not affect bone remodelling in pasture‐exercised horses

Tippen,

Metzger,

Sacks

et al. 2024

Equine Veterinary Journal

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BackgroundBisphosphonates are widely used in equine athletes to reduce lameness associated with skeletal disorders. Widespread off‐label use has led to concern regarding potential negative effects on bone healing, but little evidence exists to support or refute this.ObjectivesTo investigate the influence of clinically relevant doses of tiludronate on bone remodelling and bone healing.Study designRandomised, controlled in vivo experiments.MethodsEach horse had a single tuber coxae biopsied (Day 0), then were divided into a treatment (IV tiludronate) or control (IV saline) group. Treatments were administered 30 and 90 days following initial biopsy. Biopsy of the tuber coxae was repeated on Day 60 to evaluate bone healing following a single treatment. Oxytetracycline was administered on Days 137 and 147 to label bone formation. The contralateral tuber coxae was biopsied on Day 150 to evaluate effects of repeated treatment. Bone biopsies were evaluated with micro‐computed tomography and/or dynamic histomorphometry using standard techniques.ResultsNineteen horses completed the study, with no complications following the biopsies and treatments. No significant differences in the trabecular bone parameters or bone formation rate were observed between treatment groups.Main limitationsThe use of a first‐generation bisphosphonate may mean some effects of these drugs are underrepresented using this model. The results pertain to the tuber coxae and may not reflect injury or the healing response that occurs in long bones in training or racing.ConclusionsIn this model, tiludronate did not affect normal bone remodelling in the horse, despite repeat dosages.

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Can bone marrow aspirate improve mandibular fracture repair in camels(Camelus dromedarius)? A preliminary study

et al. 2020

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Background Mandibular fractures are common in camels, leading to considerable economic losses. This study explored methods of improving mandibular fractures repair, adjuvant with interdental wire, or bone plate fixation. Autologous bone marrow (BM) injection enhances osteogenesis and rapid healing. Objectives To investigate the effect of autologous BM aspirate as an adjuvant treatment for repairing mandibular fractures in camels with interdental wire, or bone plate fixation. Methods Thirty dromedary camels aged 5–8 years and of both sexes were randomly divided into 4 treatment groups: group 1 (n = 10) treated with stainless steel wire fixation and BM injection at the fracture line, group 2 (n = 10) treated with plate fixation and BM injection at the fracture line, group 3 (n = 5) treated with stainless steel bone wire fixation and placebo saline injection at the fracture line, and group 4 (n = 5) treated with plate fixation and placebo injection at the fracture line. The mandibular fractures were followed weekly for 12 weeks postoperatively to assess improvement and healing based on clinical evaluation, radiographic union scale, and bone turnover markers (i.e., bone alkaline phosphatase, osteocalcin, pyridinoline, and deoxypyridinoline). Results Compared to other groups, elevated bone turnover markers in group 1 were demonstrated ( p < 0.05) on the seventh postoperative day. Likewise, compared to other groups, both clinical findings and radiographic union scale significantly improved ( p < 0.05) in group 1 on the 56th postoperative day. Conclusions BM aspirate has a promising beneficial osteogenic effect on mandibular fracture repair in camels, most notably when combined with interdental wire fixation.

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Assessment of tuber coxae bone biopsy in the standing horse

Cited by 3 publications

References 7 publications

Tiludronate and clodronate do not affect bone structure or remodeling kinetics over a 60 day randomized trial

Tiludronate and clodronate do not affect bone structure or remodeling kinetics over a 60 day randomized trial

Clinically relevant doses of tiludronate do not affect bone remodelling in pasture‐exercised horses

Can bone marrow aspirate improve mandibular fracture repair in camels(Camelus dromedarius)? A preliminary study

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