Assessment of uptake and toxicity of fluorescent silica nanoparticles in zebrafish (Danio rerio) early life stages

Fent, Karl; Weisbrod, Christin J.; Wirth-Heller, Amina; Pieles, Uwe

doi:10.1016/j.aquatox.2010.02.019

Cited by 164 publications

(98 citation statements)

References 39 publications

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“…Nonetheless, this type of nanoparticle is not toxic only because it aggregates in such a way that the resulting size does not allow chorion penetration. Due to this, toxicity of the fluorescent silica nanoparticle is hard to test (Fent et al 2010). Despite the many studies found on several metal oxide NPs, results vary from work to work.…”

Section: Chen Et Al 2011mentioning

confidence: 97%

“…Studies on inorganic NP have shown that, even at sublethal concentrations, these NPs can cause alterations in the hatching rate, malformations, survival and production of reactive oxygen species (Ahmad et al 2015;Ašmonaitė et al 2016;Fang et al 2015;Ghobadian et al 2015;Hu et al 2017;Wehmas et al 2015;Zhao et al 2013). Fent et al (2010) managed to achieve a fluorescent silica nanoparticle nontoxic for zebrafish embryos. Nonetheless, this type of nanoparticle is not toxic only because it aggregates in such a way that the resulting size does not allow chorion penetration.…”

Section: Chen Et Al 2011mentioning

confidence: 99%

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Relation between biophysical properties of nanostructures and their toxicity on zebrafish

et al. 2017

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In recent years, the use of commercial nanoparticles in different industry and health fields has increased exponentially. However, the uncontrolled application of nanoparticles might present a potential risk to the environment and health. Toxicity of these nanoparticles is usually evaluated by a fast screening assay in zebrafish (Danio rerio). The use of this vertebrate animal model has grown due to its small size, great adaptability, high fertilization rate and fast external development of transparent embryos. In this review, we describe the toxicity of different micro-and nanoparticles (carbon nanotubes, dendrimers, emulsions, liposomes, metal nanoparticles, and solid lipid nanoparticles) associated to their biophysical properties using this model. The main biophysical properties studied are size, charge and surface potential due to their impact on the environment and health effects. The review also discusses the correlation of the effects of the different nanoparticles on zebrafish. Special focus is made on morphological abnormalities, altered development and abnormal behavior. The last part of the review debates changes that should be made in future directions in order to improve the use of the zebrafish model to assess nanotoxicity.

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Section: Chen Et Al 2011mentioning

confidence: 97%

Section: Chen Et Al 2011mentioning

confidence: 99%

Relation between biophysical properties of nanostructures and their toxicity on zebrafish

et al. 2017

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“…(Zhang et al 2010;Mohseni et al 2015;Ganesh and Lee 2013). Based on non-toxicity and biocompatibility, silica nanoparticles are proposed as alternative carriers for drug delivery as these can be degraded into its simple silanol units and can be removed from the body (Fent et al 2010;Yu et al 2009;Liu et al 2011). The porosity and shape of MSNPs accommodate the drug molecules.…”

Section: Introductionmentioning

confidence: 99%

Targeted delivery of mesoporous silica nanoparticles loaded monastrol into cancer cells: an in vitro study

et al. 2017

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Monastrol is a simple low molecular weight dihydropyrimidine-based kinesin Eg5 inhibitor. Its low cellular activity and its non-drug-like properties have impeded its further development. In a previous report, we have reported various topological parameters to improve the pharmacokinetic properties of monastrol. The purpose of this study is to determine the loading and release feasibility of poorly water-soluble monastrol into the synthesized mesoporous silica nanoparticles (MSNs). The synthesis of MSNs was attained by the ammonia-catalysed hydrolysis and condensation of TEOS in ethanol using polysorbate-80 as surfactant. These were characterized by BET surface area and pore size distribution analyses, SEM, XRD, UV and FTIR spectroscopy. The synthesized monastrol was successfully loaded on MSNPs and coated by hydrogels for successful controlled drug delivery. In vitro release studies are done by simple dialysis method.Monastrol-loaded MSNPs were tested on human cervical epithelial malignant carcinoma (HeLa) cell lines for studying their anticancer activity. Our presented system described a reliable method for targeted delivery of monastrol into the cancer cells in vitro.

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“…19 In addition, silica nanoparticles have not been found to have general or overt toxicity between 0.0025 mg/L and 200 mg/L. 20 Mice have been used for studying nanoparticle biology. 21 Organs that can take up nanostructures in mice include the spleen, lymph nodes, and bone marrow.…”

mentioning

confidence: 99%

Mesoporous silica nanoparticles as a compound delivery system in zebrafish embryos

Sharif¹,

Porta²,

Meijer³

et al. 2012

IJN

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Silica nanoparticles can be efficiently employed as carriers for therapeutic drugs in vitro. Here, we use zebrafish embryos as a model organism to see whether mesoporous silica nanoparticles (MSNPs) can be incorporated to deliver compounds in vivo. We injected 35-40 nL (10 mg/mL) of custom-synthesized, fluorescently-tagged 200 nm MSNPs into the left flank, behind the yolk sac extension, of 2-day-old zebrafish embryos. We tracked the distribution and translocation of the MSNPs using confocal laser scanning microscopy. Some of the particles remained localized at the injection site, whereas others entered the bloodstream and were carried around the body. Embryo development and survival were not significantly affected by MSNP injection. Acridine orange staining revealed that MSNP injections did not induce significant cell death. We also studied cellular immune responses by means of lysC::DsRED2 transgenic embryos. MSNP-injected embryos showed infiltration of the injection site with neutrophils, similar to controls injected with buffer only. In the same embryos, counterstaining with L-plastin antibody for leukocytes revealed the same amount of cellular infiltration of the injection site in embryos injected with MSNPs or with buffer only. Next, we used MSNPs to deliver two recombinant cytokines (macrophage colony-stimulating factor and receptor for necrosis factor ligand) to zebrafish embryos. These proteins are known to activate cells involved in bone remodeling, and this can be detected with the marker tartrate-resistant acid phosphatase. Coinjection of these proteins loaded onto MSNPs produced a significant increase in the number of tartrate-resistant acid phosphatase-positive cells after 2-3 days of injection. Our results show that MSNPs can be used to deliver bioactive compounds into zebrafish larvae without producing higher mortality or gross evidence of teratogenicity.

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Assessment of uptake and toxicity of fluorescent silica nanoparticles in zebrafish (Danio rerio) early life stages

Cited by 164 publications

References 39 publications

Relation between biophysical properties of nanostructures and their toxicity on zebrafish

Relation between biophysical properties of nanostructures and their toxicity on zebrafish

Targeted delivery of mesoporous silica nanoparticles loaded monastrol into cancer cells: an in vitro study

Mesoporous silica nanoparticles as a compound delivery system in zebrafish embryos

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