Assessment of urine and fecal testosterone metabolite excretion in Chinchilla lanigera males

Excretion of Steroid Hormones in Rodents: An Overview on Species Differences for New Biomedical Animal Research Models

“…In our laboratory, we have been studying aspects of reproductive biology in chinchilla (Chinchilla lanigera) for over 15 years (Ponce et al, 1998a, b;Carrascosa et al, 2002;Ponzio et al, 2004Ponzio et al, , 2007aPonzio et al, , b, 2008Busso et al, 2005aBusso et al, , b, c, 2007. This is an interesting taxon, since domesticated chinchilla used for the fur industry still share some genomic characteristics with their endangered (almost extinct) counterparts.…”

“…Similarly, information of sex steroid metabolism is essential to monitor steroidogenic activity of gonads (Brown et al, 1994). The first step in assay development is identifying the excretory routes for each hormone of interest; conducting an infusion study would be extremely valuable in domestic and nondomestic animals, if captive-held counterparts are available and the tests are feasible (Wielebnowski and Watters, 2007), such as the studies performed in domestic chinchilla to evaluate wild chinchilla (Busso et al, 2005a; see further details below). We also accept that, in practical terms, it is unnecessary to determine the specific molecular structure of the hormones being monitored in each species.…”

“…According to recent reviews, it seems that this approach, noninvasive monitoring, will be utilized more than ever when well-focused endocrine issues are addressed and some steroids are involved (Palme and Möstl, 2002;Pukazhenthi and Wildt, 2004;Palme, 2005;Schwarzenberger, 2007). In fact, international programs have trained science students, especially researches from Latin American PhD programs (Swanson and Brown, 2004) Busso et al (2005a, Ponzio et al (2004) in Argentina, of Brousset Hernández-Jauregui et al (2005) in Mexico, and of Moreira et al (2007) in Brazil. Similarly, new studies fully developed in traditionally called developing countries are clear evidence of the usefulness of this non-invasive approach (Leche et al, 2009(Leche et al, , 2011.…”

“…Therefore, based on updated endocrine data from wild, laboratory and farmed rodents, we propose to develop a comparative endocrine survey of the routes of gonadal and adrenocortical steroid excretion. In our laboratory, chinchilla were subjected to radiometabolism studies of progesterone, corticosterone, testosterone and estradiol (Ponzio Busso et al, 2005a. Since domesticated chinchilla still share some genomic characteristics with their counterparts in the wild, the analysis would serve as an adequate example of widely diverse interests (scientific, ecological, economic, cultural and emblematic values).…”

Avaliação da eficiência da eletroforese capilar como técnica analítica na prospecção de metabólitos de esteróides em extratos fecais de onça-pintada (Panthera onca)

“…A cromatografia líquida de alta performance (HPLC) vem sendo utilizada como técnica de apoio às técnicas imunométricas, permitindo a separação dos principais compostos para direcionar a escolha dos anticorpos para posterior quantificação por radioimunoensaio (RIA), enzimaimunoensaio (EIA) ou imunoadsorção enzimática (ELISA) (ZIEGLER; WITTWER, 2005;BUSSO et al, 2005;DLONIAK et al, 2004;DEHNHARD et al, 2003;HUBER et al, 2003;GOYMANN et al, 2002;WASSER et al, 2000;SCHWARZENBERGER et al, 1998;SPANNER et al, 1997;GRAHAN et al, 1995;BROWN et al, 1994;SHIDELER et al, 1993;BROWN et al, 1993;LASLEY et al, 1994;SHILLE et al, 1990;ZIEGLER et al, 1989). No entanto, principalmente por motivos econômicos, esta técnica não se estabeleceu como técnica de rotina na área de investigações por métodos não-invasivos.…”