The Middle Ogun Irrigation Project (MOIP) was conceived by the Federal Government of Nigeria to boost agricultural food production in the southwestern part of the country using modern irrigation facilities. However, water supply from the Ikere Gorge dam has been intermittent, particularly at the intake weir of this project, despite the enormous water resources on the dam. Therefore, this project assessed the water resources potential at the Odo Ogun intake weir guage station of the Middle Ogun Irrigation Project and evaluated the seasonal irrigation requirements on irrigation fields. The Flow Duration Curve (FDC) was derived at the gauge site for streamflow discharge from four quartiles and percentage reliability of Q95%, Q75%, Q50% and Q25%, which gave 32.40, 55.01, 60.00 and 90.00 m3/s, respectively. Hydrological modelling of the catchment was carried out using the MapWindow Soil and Water Assessment Tool (MWSWAT) for ten delineated sub-basins on the catchment and the average annual yield from the catchment was 10,094.00 mm/year. The net seasonal irrigation requirement of crops grown was evaluated using CROPWAT 8.0 and gave 428.46 mm per planting season.