Assessments of hepatitis B virus-like particles and Crm197 as carrier proteins in melioidosis glycoconjugate vaccines

Bayliss, Marc; Pergolizzi, Giulia; Donaldson, M.I.; Scott, Andrew E.; Nepogodiev, Sergey A.; Beales, Lucy; Whelan, Mike; Rosenberg, William; Peyret, Hadrien; Lomonossoff, George P.; Harmer, Nicholas J.; Atkins, Tim; Field, Robert A.; Prior, Joann L.

doi:10.1101/2020.04.08.031658

Cited by 2 publications

(1 citation statement)

References 55 publications

(68 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Insertion of foreign sequences into the immunodominant c/e1 B cell epitope, a surface-exposed loop on the HBV capsid protein, results in particles that have the antigenic characteristics of HBcAg and the immunogenicity of the inserted foreign epitope which is considerably enhanced. These make HBcAg one of the most promising delivery vehicles for delivering specific epitopes, such as those from HEV, to the immune system for potent vaccine production [ 39 , 40 , 41 ].…”

Section: Introductionmentioning

confidence: 99%

Efficient Production of Chimeric Hepatitis B Virus-Like Particles Bearing an Epitope of Hepatitis E Virus Capsid by Transient Expression in Nicotiana benthamiana

Zahmanova

Mazalovska

Takova

et al. 2021

Life

Self Cite

View full text Add to dashboard Cite

The core antigen of hepatitis B virus (HBcAg) is capable of self-assembly into virus-like particles (VLPs) when expressed in a number of heterologous systems. Such VLPs are potential carriers of foreign antigenic sequences for vaccine design. In this study, we evaluated the production of chimeric HBcAg VLPs presenting a foreign epitope on their surface, the 551–607 amino acids (aa) immunological epitope of the ORF2 capsid protein of hepatitis E virus. A chimeric construct was made by the insertion of 56 aa into the immunodominant loop of the HBcAg. The sequences encoding the chimera were inserted into the pEAQ-HT vector and infiltrated into Nicotiana benthamiana leaves. The plant-expressed chimeric HBcHEV ORF2 551–607 protein was recognized by an anti-HBcAg mAb and anti-HEV IgG positive swine serum. Electron microscopy showed that plant-produced chimeric protein spontaneously assembled into “knobbly” ~34 nm diameter VLPs. This study shows that HBcAg is a promising carrier platform for the neutralizing epitopes of hepatitis E virus (HEV) and the chimeric HBcAg/HEV VLPs could be a candidate for a bivalent vaccine.

show abstract

Section: Introductionmentioning

confidence: 99%

Efficient Production of Chimeric Hepatitis B Virus-Like Particles Bearing an Epitope of Hepatitis E Virus Capsid by Transient Expression in Nicotiana benthamiana

Zahmanova

Mazalovska

Takova

et al. 2021

Life

Self Cite

View full text Add to dashboard Cite

show abstract

Virus-like Particles Produced in Plants: A Promising Platform for Recombinant Vaccine Development

Mardanova,

Vasyagin,

Ravin

2024

Plants

View full text Add to dashboard Cite

The capsid proteins of many viruses are capable of spontaneous self-assembly into virus-like particles (VLPs), which do not contain the viral genome and are therefore not infectious. VLPs are structurally similar to their parent viruses and are therefore effectively recognized by the immune system and can induce strong humoral and cellular immune responses. The structural features of VLPs make them an attractive platform for the development of potential vaccines and diagnostic tools. Chimeric VLPs can be obtained by attaching foreign peptides to capsid proteins. Chimeric VLPs present multiple copies of the antigen on their surface, thereby increasing the effectiveness of the immune response. Recombinant VLPs can be produced in different expression systems. Plants are promising biofactories for the production of recombinant proteins, including VLPs. The main advantages of plant expression systems are the overall low cost and safety of plant-produced products due to the absence of pathogens common to plants and animals. This review provides an overview of the VLP platform as an approach to developing plant-produced vaccines, focusing on the use of transient expression systems.

show abstract

Assessments of hepatitis B virus-like particles and Crm197 as carrier proteins in melioidosis glycoconjugate vaccines

Abstract: 26 27

Cited by 2 publications

References 55 publications

Efficient Production of Chimeric Hepatitis B Virus-Like Particles Bearing an Epitope of Hepatitis E Virus Capsid by Transient Expression in Nicotiana benthamiana

Efficient Production of Chimeric Hepatitis B Virus-Like Particles Bearing an Epitope of Hepatitis E Virus Capsid by Transient Expression in Nicotiana benthamiana

Virus-like Particles Produced in Plants: A Promising Platform for Recombinant Vaccine Development

Contact Info

Product

Resources

About