Assignment&lt;footref rid="foot01"&gt;&lt;sup&gt;1&lt;/sup&gt;&lt;/footref&gt; of the programmed cell death 4 gene (PDCD4) to human chromosome band 10q24 by in situ hybridization

Soejima, Hidenobu; Miyoshi, Osamu; Yoshinaga, Hiroyuki; Masaki, Zenjiro; Ozaki, Iwata; Kajiwara, S; Niikawa, Norio; Matsuhashi, Sachiko; Mukai, Tsunehiro

doi:10.1159/000015408

Cited by 40 publications

(33 citation statements)

References 5 publications

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“…The mechanisms of downregulation of these genes include point mutations, loss of heterozygosity (LOH), and/or transcriptional repression by altered DNA methylation. The locus of PDCD4 is assigned to 10q24 (Soejima et al, 1999), and deletion of this region was reported in some cases of HCC (Nagai et al, 1997;Kusano et al, 1999). However, deletion of PDCD4 locus is noted only in minor cases of HCC, PDCD4 in TGF-b1-induced apoptosis H Zhang et al while PDCD4 protein levels were decreased in all cases of HCC studied here.…”

Section: Pdcd4 In Tgf-b1-induced Apoptosismentioning

confidence: 61%

“…The programmed cell death 4 (PDCD4) gene was first isolated from a human glioma cell complementary DNA (cDNA) library as a tumor-related gene Yoshinaga et al, 1997Yoshinaga et al, , 1999 and mapped to chromosome10q24 (Soejima et al, 1999). Its expression was controlled by interleukins in human natural killer (NK) and T cells (Azzoni et al, 1998).…”

Section: Introductionmentioning

confidence: 99%

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Involvement of programmed cell death 4 in transforming growth factor-β1-induced apoptosis in human hepatocellular carcinoma

et al. 2006

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The programmed cell death 4 (PDCD4) gene was originally identified as a tumor-related gene in humans and acts as a tumor-suppressor in mouse epidermal carcinoma cells. However, its function and regulatory mechanisms of expression in human cancer remain to be elucidated. We therefore investigated the expression of PDCD4 in human hepatocellular carcinoma (HCC) and the role of PDCD4 in human HCC cells. Downregulation of PDCD4 protein was observed in all HCC tissues tested compared with corresponding noncancerous liver, as revealed by Western blotting or immunohistochemical staining. Human HCC cell line, Huh7, transfected with PDCD4 cDNA showed nuclear fragmentation and DNA laddering characteristic of apoptotic cells associated with mitochondrial changes and caspase activation. Transforming growth factor-b1 (TGF-b1) treatment of Huh7 cells resulted in increased PDCD4 expression and occurrence of apoptosis, also concomitant with mitochondrial events and caspase activation. Transfection of Smad7, a known antagonist to TGF-b1 signaling, protected cells from TGF-b1-mediated apoptosis and suppressed TGF-b1-induced PDCD4 expression. Moreover, antisense PDCD4 transfectants were resistant to apoptosis induced by TGF-b1. In conclusion, these data suggest that PDCD4 is a proapoptotic molecule involved in TGF-b1-induced apoptosis in human HCC cells, and a possible tumor suppressor in hepatocarcinogenesis.

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Section: Pdcd4 In Tgf-b1-induced Apoptosismentioning

confidence: 61%

Section: Introductionmentioning

confidence: 99%

Involvement of programmed cell death 4 in transforming growth factor-β1-induced apoptosis in human hepatocellular carcinoma

et al. 2006

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“…Programmed cell death 4 (Pdcd4) is a ubiquitously expressed, novel tumor suppressor gene localized in chromosome 10q24, whose protein product plays a role in the suppression of tumorigenesis and tumor progression and invasion (1)(2)(3)(4). Although Pdcd4 was first identified as being differentially upregulated during apoptosis, experimental evidence established it as a novel tumor suppressor (5)(6)(7)(8).…”

Section: Introductionmentioning

confidence: 99%

Programmed cell death 4 (Pdcd4) expression in colorectal adenocarcinoma: Association with clinical stage

Lim

Ren

2011

Oncology Letters

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Abstract. The aim of this study was to examine the role of Programmed cell death 4 (Pdcd4) in colorectal adenocarcinoma (CRA). Pdcd4 expression was observed in both the nucleus and cytoplasm in colorectal adenocarcinoma, whereas Pdcd4 was expressed in the nucleus in normal colonic epithelial cells. Loss or weak expression of Pdcd4 was identified in 44 cases (40.7%) of cancer cells. Pdcd4 expression was associated with an increase in the nodal and clinical stage (p=0.022 and p= 0.016, respectively). Nuclear staining was identified in 66 cases (61.15%), with no correlation with clinicopathological factors. Conversely, cytoplasmic staining for Pdcd4 was observed in 45 cases (41.7%), and increased according to nodal and clinical stage (p=0.011 and p=0.009, respectively), indicating that aberrant Pdcd4 expression leads to tumor progression. However, Pdcd4 expression was not correlated to disease-free survival time. This study demonstrated that during the tumorigenesis of CRA, loss of nuclear Pdcd4 expression occurs, and during tumor progression, aberrant cytoplasmic expression is present, suggesting a higher clinical stage. Although loss of Pdcd4 was not significantly correlated with survival time, as the prognosis of colorectal cancer varies depending on clinical stage including invasion depth, nodal status and metastatic status, cytoplasmic Pdcd4 expression may be a favorable prognostic marker in CRA.

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“…The human programmed cell death 4 (PDCD4) gene (H731) was first isolated as an antigen gene involved in the cell cycle (1,2) and mapped at 10q24 (3). Homologs in mice (MA-3/TIS) (4,5), chickens (6,7) and rats (DUG) (8) have also been reported.…”

Section: Introductionmentioning

confidence: 99%

Expression patterns of the tumor suppressor PDCD4 and correlation with β-catenin expression in gastric cancers

Kakimoto

Shiraishi²,

Iwakiri³

et al. 2011

Oncol Rep

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Abstract. The expression patterns of PDCD4, a tumor suppressor, and β-catenin were immunohistologically investigated in gastric carcinoma tissues. In normal gastric tissues, PDCD4 was strongly expressed in the cell nuclei, but weakly expressed in the cytoplasm. In gastric adenocarcinoma tissues, nuclear PDCD4 expression was decreased, while cytoplasmic PDCD4 expression was unchanged or somewhat increased. In gastric signet ring cell carcinoma tissues, PDCD4 expression patterns were different from the expression patterns of the adenocarcinoma tissues, and PDCD4 was localized in the nuclei of the carcinoma cells as a belt in the middle of the epithelial layer. The nuclear localization of PDCD4 in the adenocarcinoma tissues was correlated with the membrane localization of β-catenin, the activation of which stimulates invasion of colon cancer cells. PDCD4 expression was correlated with β-catenin expression in gastric carcinoma cell lines, but not with E-cadherin, as the binding partner in the cell membrane.

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Assignment<footref rid="foot01"><sup>1</sup></footref> of the programmed cell death 4 gene (PDCD4) to human chromosome band 10q24 by in situ hybridization

Cited by 40 publications

References 5 publications

Involvement of programmed cell death 4 in transforming growth factor-β1-induced apoptosis in human hepatocellular carcinoma

Involvement of programmed cell death 4 in transforming growth factor-β1-induced apoptosis in human hepatocellular carcinoma

Programmed cell death 4 (Pdcd4) expression in colorectal adenocarcinoma: Association with clinical stage

Expression patterns of the tumor suppressor PDCD4 and correlation with β-catenin expression in gastric cancers

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