Assignment of Vairimorpha leptinotarsae comb. nov. on the basis of molecular characterization of Nosema leptinotarsae Lipa, 1968 (Microsporidia: Nosematidae)

Abstract

Nosema leptinotarsae Lipa, 1968 is a microsporidian pathogen of the Colorado potato beetle, Leptinotarsa decemlineata Say. (Coleoptera: Chrysomelidae). To determine the phylogenetic status of N. leptinotarsae, the 16S SSU rRNA gene was sequenced (GenBank Accession No. MN841279) and compared phylogenetically against 21 microsporidian 16S SSU rRNA sequences using neighbour-joining and maximum-parsimony methods. The per cent identities of the N. leptinotarsae and other members of the Nosema–Vairimorpha clade ranged from 78.1 to 98.5%. Pairwise phylogenetic distances between the N. leptinotarsae and other species ranged from 0.009 to 0.320. Phylogenetic analysis shows clearly that N. leptinotarsae is a member of the Vairimorpha clade rather than the Nosema clade. The sequence divergence and morphological traits separated the N. leptinotarsae from other species in the Vairimorpha complex. As a result, a new assignment of Vairimorpha leptinotarsae comb. nov. has been implemented for N. leptinotarsae according to the phylogenetical positioning in the present study.

Molecular Characterization of the Vairimorpha (Nosema) ceranae Infection from Bombus terrestris (Linnaeus, 1758) (Hymenoptera: Apidae) in Turkey

“…In the examinations made in this context, it was determined that the current microsporidium spores had similar features to the data presented by numerous studies previously conducted for the definition and detection of V. ceranae (Fries, Feng, da Silva, Slemenda, & Pieniazek, 1996;Chen et al, 2009). Especially in the last quarter, the microsporidial taxonomy has been constructed with the molecular phylogeny and species identifications are made on this basis (Baker, Vossbrinck, Maddox, & Undeen, 1994;Baker, Vossbrinck, Didier, Maddox, & Shadduck, 1995;Huang, Tsai, Lo, Soichi & Wang 2004;Bekircan, 2020;Tokarev et al, 2020;Tosun, 2020) 1968 (Microsporidia: Nosematidae. Therefore, in this study partial sequences of SSU rRNA and RPB1 genes of the current microsporidium were analyzed.…”

A New Microsporidian Pathogen of the Mediterranean Flour Moth, Ephestiae kuehniella (Zeller) (Lepidoptera: Pyralidae)

“…In the last quarter, molecular phylogeny has been an essential parameter for Microsporidia taxonomy. Although phylogenetic analyzes based on SSU rRNA are insufficient to reveal the current situation in some genera, it is still widely used in the microsporidian phylogeny [7,21]. Phylogenetic comparison of the current microsporidium with V. necatrix strains based on SSU rRNA showed a significant genetic difference (2.72-2.79%).…”

“…In recent years, other combat techniques such as microbial control have attracted attention in pest control. Entomopathogenic organisms that cause infection in pests such as E. kuehniella frequently recorded in the literature, and there is increasing interest in isolating and identifying these organisms [6,7,8,9,10,11].…”

A new isolate ofNosema fumiferanae(Microsporidia: Nosematidae) from the date mothApomyelois(Ectomyelois)ceratoniae, Zeller, 1839 (Lepidoptera: Pyralidae)

“…However, the date moth is widely controlled with different pesticides like methyl bromide which is highly toxic and poses several hazards to animals and humans (Hallier et al ., 1990). Using chemicals against pests affects the environment and non-target organisms negatively, and these effects have led to new approaches, especially in the last quarter, to identify the natural pathogens of pests (Bekircan et al ., 2017; Bekircan, 2020).…”