Assignment of the gene for cytoplasmic glutamic-oxaloacetic transaminase to the region q24-qter of human chromosome 10

Abstract

Somatic cell hybrids between thymidine kinase-deficient mouse cells and human fibroblasts carrying a translocation of the distal third of the long arm of chromosome 10 to chromosome 17 were studied for the expression of cytoplasmic glutamic-oxaloacetic transaminase. A positive correlation between the expression of human cytoplasmic glutamic-oxaloacetic transaminase and the presence of the distal third of the long arm of chromosome 10 was established.

Glycosaminoglycan Degradation

“…The biological role of P-glucuronidase in the catabolism of hyaluronate, chondroitin sulfate, dermatan sulfate, heparan sulfate, and heparin is best established by the existence of a human storage disorder, mucopolysaccharidosis VII, which is caused by an inactivity of this enzyme (134,135). The disorder is due to a mutation in the structural gene of P-glucuronidase (179), which is localized on chromosome 7 (180). Clinical manifestations of the disorder include mental retardation, hepatosplenomegaly , progressive skeletal deformities, and excessive urinary excretion of the respective glycosaminoglycan fragments.…”

Mapping <i>AK₁ ACO</i><i>N</i>_s, and <i>AK</i>₃ to chromosome 9 in man employing an X/9 translocation and somatic cell hybrids

“…This same strategy applies if a selectable marker is translocated to another chromosome. Markers on such a translocation chromosome can be mapped employing the appro priate selection-counterselection methodology (Ricciuri and Ruddle, 1973;Chern et al, 1976).…”

Localization of the structural genes for hexokinase-1 and inorganic pyrophosphatase on region (pter→q24) of human chromosome 10

“…Human skin fibroblasts with the balanced translocation 46,XY,t(10;17)(10pter-► I0q24::17pl3-17pter [der (10)l;17qter--17pl3:: 10q24-»-lOqter [der (17)]) (Zackai et al, 1975) were fused with TK-deficient Chinese hamster cells (As) in the presence of /f-propiolactone-inactivated Sendai virus (Chern et al, 1976). The hybrid cells were maintained in HAT medium in order to retain either the der (17) chromo some or the normal chromosome 17.…”