Assimilation of chlorinated alkanes by hydrocarbon-utilizing fungi

Murphy, George L.; Perry, Jerome J.

doi:10.1128/jb.160.3.1171-1174.1984

Cited by 29 publications

(7 citation statements)

References 19 publications

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“…Fungal metabolization of hydrocarbon pollutants is predominantly aerobic. A limited number of simple aromatic and aliphatic hydrocarbons such as n-alkanes, n-alkylbenzenes, aliphatic ketones, ethylbenzene, styrene and toluene can be used as sole sources of carbon and energy for growth of filamentous fungi (Gerasimova et al, 1975;Murphy & Perry, 1984;Fedorak & Westlake, 1986;Weber et al, 1995;Qi & Moe, 2002) and yeasts (Jigami et al, 1974;Cox et al, 1993Cox et al, , 1996Craft et al, 2003). Mitosporic fungi (Hofrichter & Scheibner, 1993;Jones et al, 1993Jones et al, , 1994García-Peña et al, 2005) and yeasts (Middelhoven & Spaaij, 1997;Middelhoven et al, 2004) are able to use phenol, o-cresol, m-cresol, p-cresol and 4ethylphenol as growth substrates.…”

Section: Distinctive Features Of Fungal Organopollutant Metabolism Anmentioning

confidence: 99%

Fungi in freshwaters: ecology, physiology and biochemical potential

et al. 2011

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Research on freshwater fungi has concentrated on their role in plant litter decomposition in streams. Higher fungi dominate over bacteria in terms of biomass, production and enzymatic substrate degradation. Microscopy-based studies suggest the prevalence of aquatic hyphomycetes, characterized by tetraradiate or sigmoid spores. Molecular studies have consistently demonstrated the presence of other fungal groups, whose contributions to decomposition are largely unknown. Molecular methods will allow quantification of these and other microorganisms. The ability of aquatic hyphomycetes to withstand or mitigate anthropogenic stresses is becoming increasingly important. Metal avoidance and tolerance in freshwater fungi implicate a sophisticated network of mechanisms involving external and intracellular detoxification. Examining adaptive responses under metal stress will unravel the dynamics of biochemical processes and their ecological consequences. Freshwater fungi can metabolize organic xenobiotics. For many such compounds, terrestrial fungal activity is characterized by cometabolic biotransformations involving initial attack by intracellular and extracellular oxidative enzymes, further metabolization of the primary oxidation products via conjugate formation and a considerable versatility as to the range of metabolized pollutants. The same capabilities occur in freshwater fungi. This suggests a largely ignored role of these organisms in attenuating pollutant loads in freshwaters and their potential use in environmental biotechnology.

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Section: Distinctive Features Of Fungal Organopollutant Metabolism Anmentioning

confidence: 99%

Fungi in freshwaters: ecology, physiology and biochemical potential

et al. 2011

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“…Yarrowia lipolytica is a non-pathogenic aerobic yeast species widely used in industrial applications such as citric acid production, peach flavor production and enzyme production (protease, RNase, lipase). Many researchers reported that Yarrowia lipolytica could degrade nitraromatic compound TNT (2,4,6-Trinitrotoluene), halogenated alkanes, triglycerides, aliphatic and aromatic hydrocarbons [17][18][19][20][21][22][23].…”

Section: Introductionmentioning

confidence: 99%

Decolorization of Reactive Black 5 by Yarrowia lipolytica NBRC 1658

Aracagök¹,

Cihangir²

2013

AJMR

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Yarrowia lipolytica NBRC 1658 could decolorize Reactive Black 5 effectively through biodegradation. The optimum conditions such as initial pH, glucose concentration, nitrogen concentration and initial dye concentration were determined. Correlations between decolorization and laccase and manganese peroxidase activities were investigated. Neither laccase nor manganese peroxidase (MnP) activities were determined in culture mediums. Y. lipolytica could decolorize 97% percentage of 50mg l-1 RB5 dye within 24 hours and could tolerate up to 300 mg l-1 of dye. It was found that decolorization occurred during the exponential growth phase. Aerobic batch conditions with 5g l-1 glucose and 1g l-1 ammonium sulphate at pH 7 were considered to be the optimum decolorizing conditions.

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“…By comparison, 1-haloalkanes, which are the simplest compounds of this chemical family, are less recalcitrant to biodegradation. Thus, several strains of fungi, bacteria, and protozoa capable of using hydrocarbons as sole source of carbon are also known to degrade 1-chloroalkanes and to incorporate the resulting FA into their cellular lipids (8)(9)(10)(11)(12). In this paper we describe the isolation and the characterization of metabolites formed during the growth of Marinobacter hydrocarbonoclasticus, strain SP17, on 1-chlorooctadecane.…”

mentioning

confidence: 99%

Incorporation of 1‐chlorooctadecane into FA and β‐hydroxy acids of Marinobacter hydrocarbonoclasticus

Aubert

Metzger

Largeau

2004

Lipids

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The lipids of the gram-negative marine bacterium Marinobacter hydrocarbonoclasticus, cultivated in synthetic seawater supplemented with 1-chlorooctadecane as sole source of carbon, were isolated, purified, and their structures determined. Three pools of lipids were isolated according to the sequential procedure used: unbound lipids extracted by organic solvents, ester-bound lipids released under alkaline conditions, and amide-bound lipids released by acid hydrolysis. FA isolated from the unbound lipids included omega-chlorinated (21%, w/w, of this fraction; C16 predominant) and nonchlorinated compounds (22%, w/w; C18 predominant). These acids were accompanied by a high proportion of omega-chloro-C18 alcohols (43%, w/w) and a lower amount of omega-chloro-beta-hydroxy-C18, -C16, and -C14 acids (5%, w/w). These data, together with the isolation from the culture medium of gamma-butyrolactone, suggested a metabolism of 1-chlorooctadecane through oxidation into omega-chloro acid and then the classic beta-oxidation pathway. The analysis of the ester-bound and amide-bound lipids revealed that significant amounts of omega-chloro-beta-hydroxy C10-C12 acids were incorporated into the lipopolysaccharides of the bacterium. Incorporation of these omega-chloro-beta-hydroxy acids into the lipopolysaccharides represents a novel route for chloroalkane assimilation in hydrocarbonoclastic gram-negative bacteria. The formation of chlorinated hydroxy acids, like the omega-chloro FA in the cellular lipids, could account for an incomplete mineralization of chloroparaffins in the environment.

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Assimilation of chlorinated alkanes by hydrocarbon-utilizing fungi

Cited by 29 publications

References 19 publications

Fungi in freshwaters: ecology, physiology and biochemical potential

Fungi in freshwaters: ecology, physiology and biochemical potential

Decolorization of Reactive Black 5 by <i>Yarrowia</i><i> </i><i>l</i><i>ipolytica</i> NBRC 1658

Incorporation of 1‐chlorooctadecane into FA and β‐hydroxy acids of Marinobacter hydrocarbonoclasticus

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