Assimilation of methylamine by Paracoccus denitrificans involves formaldehyde transport by a specific carrier

KOSTLER, M; Kleiner, Diethelm

doi:10.1016/0378-1097(89)90356-x

Cited by 11 publications

(5 citation statements)

References 12 publications

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“…Methylamine is split into ammonia and formaldehyde by MADH ; formaldehyde is subsequently transported across the cytoplasmic membrane (Kostler and Kleiner, 1989). In the cytoplasm, formaldehyde is oxidized to formate by formaIdehyde dehydrogenase (Ras et al, 1995), and formate is oxidized to carbon dioxide by formate dehydrogenase.…”

Section: Resultsmentioning

confidence: 99%

The Oxidation of Methylamine in Paracoccus denitrificans

Gier

Oost

Harms

et al. 1995

European Journal of Biochemistry

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The in vivo oxidation of methylamine has been studied in Paracoccus denitrificans. Four components are involved in the electron transfer from methylamine to oxygen; methylamine dehydrogenase (MADH), amicyanin, cytochrome c and cytochrome-c oxidase. In P. denitrificans, MADH and its electron acceptor amicyanin are indispensable for growth on methylamine. In the present study, site-directed mutants have been used to demonstrate participation of cytochrome c550 and the aa3-type cytochrome-c oxidase. Moreover, evidence is provided for the operation of alternative routes, branching from amicyanin, in which at least cytochrome c1 and the cbb3-type cytochrome-c oxidase are involved.

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Section: Resultsmentioning

confidence: 99%

The Oxidation of Methylamine in Paracoccus denitrificans

Gier

Oost

Harms

et al. 1995

European Journal of Biochemistry

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“…AMPA is cleaved to produce inorganic phosphate and methylamine. Although it has been stated that methylamine is ultimately mineralized to CO 2 and NH 3 , [25], the presence of methylamine dehydrogenase in soil bacteria can convert methylamine to formaldehyde [26]. The specific kinetics, relative to the dynamics of these five biomolecules and their molecular interactions, are derived from the literature and provided in the Supplementary Materials in Table B1 along with the references.…”

Section: Identification Of Mechanisms Of Glyphosate Catabolism and Glmentioning

confidence: 99%

<i>In-Silico</i> Analysis & <i>In-Vivo</i> Results Concur on Glutathione Depletion in Glyphosate Resistant GMO Soy, Advancing a Systems Biology Framework for Safety Assessment of GMOs

Ayyadurai¹,

Hansén²,

Fagan³

et al. 2016

AJPS

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This study advances previous efforts towards development of computational systems biology, in silico, methods for biosafety assessment of genetically modified organisms (GMOs). C1 metabolism is a critical molecular system in plants, fungi, and bacteria. In our previous research, critical molecular systems of C1 metabolism were identified and modeled using CytoSolve ® , a platform for in silico analysis. In addition, multiple exogenous molecular systems affecting C1 metabolism such as oxidative stress, shikimic acid metabolism, glutathione biosynthesis, etc. were identified. Subsequent research expanded the C1 metabolism computational models to integrate oxidative stress, suggesting glutathione (GSH) depletion. Recent integration of data from the EPSPS genetic modification of Soy, also known as Roundup Ready Soy (RRS), with C1 metabolism predicts similar GSH depletion and HCHO accumulation in RRS. The research herein incorporates molecular systems of glutathione biosynthesis and glyphosate catabolism to expand the extant in silico models of C1 metabolism. The in silico results predict that Organic Soy will have a nearly 250% greater ratio of GSH and GSSG, a measure of glutathione levels, than in RRS that are glyphosate-treated glyphosate-resistant Soy versus the Organic Soy. These predictions also concur with in vivo greenhouse results. This concurrence suggests that these in silico models of C1 metabolism may provide a viable and validated platform for biosafety assessment of GMOs, and aid in selecting rational criteria for informing in vitro and in vivo efforts to more accurately decide in the problem formulation * Corresponding author. V. A. Shiva Ayyadurai et al. 1572phase whose parameters need to be assessed so that conclusion on "substantial equivalence" or material difference of a GMO and its non-GMO counterpart can be drawn on a well-grounded basis.

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“…The oxidation product of both methanol and methylamine is formaldehyde, which is translocated across the membrane to the cytoplasm. This transport process has been suggested to involve a specific formaldehyde carrier (159). In the cytoplasm, formaldehyde is further oxidized to formate via two consecutive reactions.…”

Section: Genes For Autotrophymentioning

confidence: 99%

Molecular Genetics of the GenusParacoccus: Metabolically Versatile Bacteria with Bioenergetic Flexibility

Baker¹,

Ferguson

Ludwig

et al. 1998

Microbiol Mol Biol Rev

211

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SUMMARY Paracoccus denitrificans and its near relative Paracoccus versutus (formerly known as Thiobacilllus versutus) have been attracting increasing attention because the aerobic respiratory system of P. denitrificans has long been regarded as a model for that of the mitochondrion, with which there are many components (e.g., cytochrome aa3 oxidase) in common. Members of the genus exhibit a great range of metabolic flexibility, particularly with respect to processes involving respiration. Prominent examples of flexibility are the use in denitrification of nitrate, nitrite, nitrous oxide, and nitric oxide as alternative electron acceptors to oxygen and the ability to use C1 compounds (e.g., methanol and methylamine) as electron donors to the respiratory chains. The proteins required for these respiratory processes are not constitutive, and the underlying complex regulatory systems that regulate their expression are beginning to be unraveled. There has been uncertainty about whether transcription in a member of the alpha-3 Proteobacteria such as P. denitrificans involves a conventional ς70-type RNA polymerase, especially since canonical −35 and −10 DNA binding sites have not been readily identified. In this review, we argue that many genes, in particular those encoding constitutive proteins, may be under the control of a ς70 RNA polymerase very closely related to that of Rhodobacter capsulatus. While the main focus is on the structure and regulation of genes coding for products involved in respiratory processes in Paracoccus, the current state of knowledge of the components of such respiratory pathways, and their biogenesis, is also reviewed.

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Assimilation of methylamine by Paracoccus denitrificans involves formaldehyde transport by a specific carrier

Cited by 11 publications

References 12 publications

The Oxidation of Methylamine in Paracoccus denitrificans

The Oxidation of Methylamine in Paracoccus denitrificans

<i>In-Silico</i> Analysis & <i>In-Vivo</i> Results Concur on Glutathione Depletion in Glyphosate Resistant GMO Soy, Advancing a Systems Biology Framework for Safety Assessment of GMOs

Molecular Genetics of the GenusParacoccus: Metabolically Versatile Bacteria with Bioenergetic Flexibility

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Assimilation of methylamine by Paracoccus denitrificans involves formaldehyde transport by a specific carrier

Cited by 11 publications

References 12 publications

The Oxidation of Methylamine in Paracoccus denitrificans

The Oxidation of Methylamine in Paracoccus denitrificans

&lt;i&gt;In-Silico&lt;/i&gt; Analysis &amp; &lt;i&gt;In-Vivo&lt;/i&gt; Results Concur on Glutathione Depletion in Glyphosate Resistant GMO Soy, Advancing a Systems Biology Framework for Safety Assessment of GMOs

Molecular Genetics of the GenusParacoccus: Metabolically Versatile Bacteria with Bioenergetic Flexibility

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<i>In-Silico</i> Analysis & <i>In-Vivo</i> Results Concur on Glutathione Depletion in Glyphosate Resistant GMO Soy, Advancing a Systems Biology Framework for Safety Assessment of GMOs