Assimilatory reduction of sulfate and sulfite by methanogenic bacteria

Daniels, Lacy; Belay, Negash; Rajagopal, B. S.

doi:10.1128/aem.51.4.703-709.1986

Cited by 154 publications

(58 citation statements)

References 35 publications

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“…Sulfate assimilation has been reported for Methanococcus thermolithotrophicus (Daniels et al, 1986), and recently all genes necessary for the reduction of sulfate to sulfide have been identified in the genome of the methanogenic archaeon RC-IMRE50 (Erkel et al, 2006). However, assimilatory sulfate reduction is unlikely carried out by ANME-1 in sulfidic AOM habitats as it would be energetically more favourable to assimilate sulfur from H2S.…”

Section: Sulfur Metabolismmentioning

confidence: 99%

Metagenome and mRNA expression analyses of anaerobic methanotrophic archaea of the ANME‐1 group

Meyerdierks

Kube

Kostadinov

et al. 2010

Environmental Microbiology

242

354

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Microbial consortia mediating the anaerobic oxidation of methane with sulfate are composed of methanotrophic Archaea (ANME) and Bacteria related to sulfate-reducing Deltaproteobacteria. Cultured representatives are not available for any of the three ANME clades. Therefore, a metagenomic approach was applied to assess the genetic potential of ANME-1 archaea. In total, 3.4 Mbp sequence information was generated based on metagenomic fosmid libraries constructed directly from a methanotrophic microbial mat in the Black Sea. These sequence data represent, in 30 contigs, about 82-90% of a composite ANME-1 genome. The dataset supports the hypothesis of a reversal of the methanogenesis pathway. Indications for an assimilatory, but not for a dissimilatory sulfate reduction pathway in ANME-1, were found. Draft genome and expression analyses are consistent with acetate and formate as putative electron shuttles. Moreover, the dataset points towards downstream electron-accepting redox components different from the ones known from methanogenic archaea. Whereas catalytic subunits of [NiFe]-hydrogenases are lacking in the dataset, genes for an [FeFe]-hydrogenase homologue were identified, not yet described to be present in methanogenic archaea. Clustered genes annotated as secreted multiheme c-type cytochromes were identified, which have not yet been correlated with methanogenesis-related steps. The genes were shown to be expressed, suggesting direct electron transfer as an additional possible mode to shuttle electrons from ANME-1 to the bacterial sulfate-reducing partner.

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Section: Sulfur Metabolismmentioning

confidence: 99%

Metagenome and mRNA expression analyses of anaerobic methanotrophic archaea of the ANME‐1 group

Meyerdierks

Kube

Kostadinov

et al. 2010

Environmental Microbiology

242

354

View full text Add to dashboard Cite

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“…All experiments were performed using ATCC 1191 medium as described (Sparling et al, 2006) with 2 g L À1 cellobiose unless otherwise specified. Bottles containing medium were sealed using butyl rubber stoppers and made anaerobic through gassing : degassing (1 min : 4 min) four times with a final gas cycle using 100% N 2 in accordance with established protocols (Daniels et al, 1986). Reducing solution, 200 mM sodium sulfide, was added at 1% (v/v) prior to autoclaving.…”

Section: Media and Substratesmentioning

confidence: 99%

Isolates of Thermoanaerobacter thermohydrosulfuricus from decaying wood compost display genetic and phenotypic microdiversity

Verbeke

Dumonceaux

Wushke

et al. 2011

FEMS Microbiol Ecol

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In this study, 12 strains of Thermoanaerobacter were isolated from a single decaying wood compost sample and subjected to genetic and phenotypic profiling. The 16S rRNA encoding gene sequences suggested that the isolates were most similar to strains of either Thermoanaerobacter pseudethanolicus or Thermoanaerobacter thermohydrosulfuricus. Examination of the lesser conserved chaperonin-60 (cpn60) universal target showed that some isolates shared the highest sequence identity with T. thermohydrosulfuricus; however, others to Thermoanaerobacter wiegelii and Thermoanaerobacter sp. Rt8.G4 (formerly Thermoanaerobacter brockii Rt8.G4). BOX-PCR fingerprinting profiles identified differences in the banding patterns not only between the isolates and the reference strains, but also among the isolates themselves. To evaluate the extent these genetic differences were manifested phenotypically, the utilization patterns of 30 carbon substrates were examined and the niche overlap indices (NOI) calculated. Despite showing a high NOI (> 0.9), significant differences existed in the substrate utilization capabilities of the isolates suggesting that either a high degree of niche specialization or mechanisms allowing for non-competitive co-existence, were present within this ecological context. Growth studies showed that the isolates were physiologically distinct in both growth rate and the fermentation product ratios. Our data indicate that phenotypic diversity exists within genetically microdiverse Thermoanaerobacter isolates from a common environment.

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“…Mb. thermoautotrophicum strain Marburg (DSM 2133) was grown at 60°C as previously described [7]. Aerobic manipulations of cells required pretreatment of the cells with N2, while anaerobic ones involved an anaerobic chamber (Coy Laboratory Prod., MI).…”

Section: Growth and Cultivationmentioning

confidence: 99%

Electron transfer reactions for the reduction of NADP+ in Methanosphaera stadtmanae

Wong¹

1994

FEMS Microbiology Letters

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Methanosphaera stadtmanae, a member of the Methanobacteriales reduces methanol, but not CO 2 with H 2 or 2-propanol to produce methane. In cell-free extracts of M. stadtmanae the activities of several enzymes involved in electron transfer were measured. The activities of an F420-nonreactive hydrogenase, NADP + :F42 o oxidoreductase, NADP+-dependent 2-propanol dehydrogenase, and a methyl viologen dependent F4z o dehydrogenase were observed. Based on the presence of these particular enzyme activities, their cofactor requirements and the absence of F42o-dependent hydrogenase activity, a model of the electron transport pathway through the coenzyme 1=42 o to provide electrons for biosynthesis, was formulated.

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Assimilatory reduction of sulfate and sulfite by methanogenic bacteria

Cited by 154 publications

References 35 publications

Metagenome and mRNA expression analyses of anaerobic methanotrophic archaea of the ANME‐1 group

Metagenome and mRNA expression analyses of anaerobic methanotrophic archaea of the ANME‐1 group

Isolates of Thermoanaerobacter thermohydrosulfuricus from decaying wood compost display genetic and phenotypic microdiversity

Electron transfer reactions for the reduction of NADP+ in Methanosphaera stadtmanae

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