Assisted Hatching of Human Embryos for Successful Implantation

Avella, Matteo A.; Milne, Kristine A.; Dawood, Shagufta; Dawood, A A; Tucker, Michael

doi:10.1007/978-3-319-43011-9_46

Cited by 4 publications

(4 citation statements)

References 109 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The AH procedure is usually carried out on embryos that are of good quality, as it is believed that embryos of poor quality will not benefit from it. It is also used in cases where the ZP is thickened or the embryo has not been successfully implanted after previous attempts at embryo transfer [ 12 , 13 ].…”

Section: Discussionmentioning

confidence: 99%

A Case Study on the Efficacy of Hypo-Osmotic Swelling Test and Assisted Hatching in Overcoming Necrozoospermia Challenges

Khemani,

More,

Shrivastava

et al. 2024

Cureus

View full text Add to dashboard Cite

This case report examines the difficulties faced by a couple with 11 years of primary infertility. Based on the diagnostic evaluation, it was determined that the male was a necrozoospermia patient, while the female had unilateral cornual blockage and polycystic ovarian syndrome (PCOS) with diabetes mellitus (DM) symptoms identified. A comprehensive approach was used in the treatment for the female patient, which included a gonadotropin-releasing hormone (GnRH) short antagonist protocol, a GnRH agonist (GnRHa) trigger, assisted hatching (AH), and the use of the hypo-osmotic swelling test (HOST) to gauge the viability of the sperm. The successful outcome, as evidenced by the increasing levels of beta-human chorionic gonadotropin (β-hCG) and a successful embryo transfer, highlights the effectiveness of a customized and multifaceted approach in managing intricate infertility problems. This instance offers insightful information about the way modern reproductive technologies can be successfully integrated with specialized treatment plans to achieve successful outcomes in difficult cases of infertility.

show abstract

Section: Discussionmentioning

confidence: 99%

A Case Study on the Efficacy of Hypo-Osmotic Swelling Test and Assisted Hatching in Overcoming Necrozoospermia Challenges

Khemani,

More,

Shrivastava

et al. 2024

Cureus

View full text Add to dashboard Cite

show abstract

“…However, the present study also identified an extensive disparity in the practice of AH. Traditionally, AHpET is mostly widely performed at the cleavage stage [ 32 ]. However, in the present study, 42.6% of centres preformed AHpET at the blastocyst stage.…”

Section: Discussionmentioning

confidence: 99%

The Current Practice of Assisted Hatching for Embryos in Fertility Centres: a General Survey

2022

View full text Add to dashboard Cite

At present, there is no standardised protocol for assisted hatching (AH) and the field is beset with contradictory data. We hypothesised that such contradiction may be related to inconsistencies in clinical practice. This study aimed to investigate the application, preferences, and variations of AH in current clinical practice prior to embryo transfer (AHpET) and biopsy (AHpBP). An online voluntary survey, consisted of 25 questions regarding different aspects of AH, was circulated amongst different fertility centres via newsletters between October 2019 and March 2020. One-hundred twenty-nine different fertility centres participated in the survey. AHpBP was widely used (90.6% [48/53]) amongst these centres, especially for trophectoderm biopsy (92.2% [47/51]). In contrast, only 64.6% (73/113) of centres administrated AHpET; the application of AHpET was even lower in UK-based centres (36.6% [15/41]). Although laser pulses have become the predominant technique for AH, significant variation existed in the precise strategy. Zona pellucida (ZP) drilling was the main method for AHpBP, whilst both ZP drilling and ZP thinning were applied equally for AHpET. Furthermore, the ZP manipulation varied widely with regards to the size of the ZP opening and the extension of ZP thinning. This is the first representative survey relating to the current practice of AH. Laser-assisted AH is used extensively, especially for AHpBP. However, there is significant disparity in clinical practice across different centres. Future research should aim to create a standardised protocol for AH to help reduce the evident variation in clinical practice and investigate the true value of AH.

show abstract

“…Many techniques had been used in intracytoplasmic sperm injection (ICSI) to improve endometrial receptivity [1] but also the embryo quality has an important role in pregnancy occurrence It has been found that embryo cryopreservation increases the cumulative pregnancy rate in ICSI cycles [2]. Zona pellucida (ZP) hardening as a consequence to cryopreservation can impair embryo hatching and in turn lowering the implantation rate [3]. The presence of fewer and smaller holes in the zona pellucida (ZP) of cryopreserved embryos compared to those found in fresh ZPs could alter the hatching process [4].…”

Section: Introductionmentioning

confidence: 99%

Laser assisted thinning versus drilling in cryopreserved embryo transfer cycles

Elnahas,

Abdel Rasheed,

Nasr

et al. 2024

Ital J Gynaecol Obstet

View full text Add to dashboard Cite

Objective. Human embryo cryopreservation is a crucial aspect of Assisted Reproduction (AR). Nonetheless there is a potential concern regarding zonal hardening, which may have a negative impact on AR outcome. Assisted hatching (AH) has been employed to facilitate microdissection of the zona pellucida. Both laser assisted thinning and drilling techniques have been used to address this issue. However insufficient studies compared laser thinning versus opening technique. We aimed to study the effect of laser assisted hatching on cryopreserved embryos, comparing both laser assisted thinning and laser assisted drilling techniques and their effects on both implantation rate chemical and clinical pregnancies.Patients and Methods. 300 women were randomized into 3 equal groups (thinning, drilling and control group) and prepared for transfer of frozen/thawed embryos. The thinning and opening techniques were performed on the day of embryo transfer while the zona of the control group was left intact. M a n u s c r i p t a c c e p t e d f o r p u b l i c a t i o nResults. Both the chemical and clinical pregnancy rates were higher in laser-thinning group over the control group in all women enrolled in the study (p-value, 0.04, 0.031 respectively) and specifically above age of 35 years old (p-value, 0.003, 0.006 respectively) with a significant implantation rate (p-value 0.043) on addition. However, laser-drilling group showed no advantage over neither the control group or the thinning group. Conclusions.Laser thinning technique for cryopreserved embryos increased both the chemical and clinical pregnancy rates in frozen embryo transfer especially above age of 35 years old and it seems to have the upper hand in relation to laser opening technique.

show abstract

Assisted Hatching of Human Embryos for Successful Implantation

Cited by 4 publications

References 109 publications

A Case Study on the Efficacy of Hypo-Osmotic Swelling Test and Assisted Hatching in Overcoming Necrozoospermia Challenges

A Case Study on the Efficacy of Hypo-Osmotic Swelling Test and Assisted Hatching in Overcoming Necrozoospermia Challenges

The Current Practice of Assisted Hatching for Embryos in Fertility Centres: a General Survey

Laser assisted thinning versus drilling in cryopreserved embryo transfer cycles

Contact Info

Product

Resources

About