Assistive technologies (ATs) often have a high-dimensionality of possible movements (e.g., assistive robot with several degrees of freedom or a computer), but the users have to control them with low-dimensionality sensors and interfaces (e.g., switches). This paper presents the development of an open-source interface based on a sequence-matching algorithm for the control of ATs. Sequence matching allows the user to input several different commands with low-dimensionality sensors by not only recognizing their output, but also their sequential pattern through time, similarly to Morse code. In this paper, the algorithm is applied to the recognition of hand gestures, inputted using an inertial measurement unit worn by the user. An SVM-based algorithm, that is aimed to be robust, with small training sets (e.g., five examples per class) is developed to recognize gestures in real-time. Finally, the interface is applied to control a computer’s mouse and keyboard. The interface was compared against (and combined with) the head movement-based AssystMouse software. The hand gesture interface showed encouraging results for this application but could also be used with other body parts (e.g., head and feet) and could control various ATs (e.g., assistive robotic arm and prosthesis).