Associated resistance of Escherichia coli isolated from humans and animals to polymyxin and beta-lactam antibiotics

Sulian, O. S.; Ageevets, Vladimir; Sukhinin, A. A.; Lazareva, I.; Abgaryan, S. R.; Makavchik, S.A.; Каменева, О. А.; Kosyakova, K. G.; Мругова, Т. М.; Popov, D. A.; Punchenko, O. E.; Сидоренко, С В

doi:10.37489/0235-2990-2021-66-11-12-9-17

Cited by 11 publications

(5 citation statements)

References 44 publications

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“…При подборе дисков также учитывали перекрёстную резистентность к нескольким антибиотикам сразу. То есть, согласно экспертным правилам интерпретации устойчивость к ампициллину для E. coli и P. mirabilis распространяется и на пиперациллин; чувствительность энтеробактерий к ципрофлоксацину распространяется на все фторхинолоны [4,6]. Чувствительность к цефалоспоринам (в нашем исследовании мы использовали цефтазидим) зачастую может являться перекрёстной и распространяться на весь ряд.…”

Section: материалы и методыunclassified

Antibiotic resistance of microbiota of drinking milk sold in St.Petersburg and Leningrad region

Noskov,

Prikhodko,

Martynova

2023

jour

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In view of the increasing interest in the issues of antibiotic resistance of opportunistic microorganisms, there is a need for a closer study of bacteria isolated from animal products. Such bacteria can carry genes for resistance to antimicrobial drugs, including those used to treat human diseases [4, 10]. In this case, drinking milk is of particular interest, because the antibiotic-resistant bacteria contained in it can freely enter the body in the absence of additional heat treatment before consumption. The work took into account the requirements of the Technical Regulations of the Customs Union (TR CU) 021/2011 "On food safety" and TR CU 033/2013 "On the safety of milk and dairy products", as well as the recommendations of the European Committee for Antimicrobial Susceptibility Testing EUCAST and the Interregional Association for clinical microbiology and antimicrobial chemotherapy MACMAC. As a result of the research, the presence of resistance to antibacterial drugs was determined in 36 bacterial isolates from milk sold in St. Petersburg and the Leningrad Region. It was found that the most common resistance to ampicillin (24 cases) and tetracycline (6 resistant isolates, 8 with intermediate values). There were also cases of resistance and intermediate results of isolates in relation to other antibiotics, including cephalosporins.

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Section: материалы и методыunclassified

Antibiotic resistance of microbiota of drinking milk sold in St.Petersburg and Leningrad region

Noskov,

Prikhodko,

Martynova

2023

jour

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“…Определение чувствительности и резистентности фенотипическими методами проводится с помощью: диск-диффузионного метода, метода микроразведения в бульоне, метода разведения в агаре, метода Е-тестов [1,9,12].…”

Section: результаты и обсуждениеunclassified

Laboratory methods for monitoring resistance to methycillin of Staphylococcus of zoonotic origin

Makavchik

Borisova

2023

jour

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The development of antibiotic resistance in microorganisms is considered a modern phenomenon, which is usually caused by empirical antibiotic therapy. One of the representatives with high antibiotic resistance is staphylococcus aureus.Staphylococcus aureus is a pathogen resistant to broad-spectrum antibiotics and is responsible for most nosocomial infections. This microorganism synthesizes penicillinase, an enzyme that breaks down penicillin molecules. To effectively combat Staphylococcus aureus, a modified penicillin, methicillin, was previously used, the molecule of which was not destroyed by this enzyme. However, methicillin-resistant strains of this pathogen are also known today.As part of the group of methicillin-resistant staphylococci (MRSA), there are three subgroups: nosocomial variants of MRSA, community-acquired MRSA, MRSA associated with farm animals, common among farm animals (livestockassociated MRSA, LA-MRSA). MRSA are characterized by multidrug resistance.It was previously believed that Staphylococcus intermedius is the most dangerous staphylococcus for pets. However, over the past few years it has been found that the most common is Staphylococcus pseudintermedius, which is gaining increasing relevance. Staphylococcus pseudintermedius (MRSP) is a bacterium commonly found on the skin, mouth, nasal mucosa, and gastrointestinal tract in approximately 50% of pets. Unlike MRSA, MRSP is much more common in dogs and cats. One of the main methods of laboratory control over their spread is a monitoring study of the epizootic and epidemiological situation among animals and the population.The home environment plays a major role in the transmission of MRSA and MRSP, therefore, by taking careful measures to prevent the spread of infection, the likelihood of infection in both companion animals and humans can be reduced.The determination of sensitivity and resistance by phenotypic methods is carried out using: disk diffusion method, broth microdilution method, agar dilution method, E-test method.Laboratory methods for monitoring the mechanisms of resistance of staphylococci to methicillin include phenotypic methods using disks with oxacillin, or cefoxitin.Genotypic methods using polymerase chain reaction allow detecting the mecA, mecC gene.For a serological diagnostic method, a latex agglutination test is used to identify MRSA and MSSA (PSB2a / PSB2 spicelin-binding protein).Treatment of animals requires special attention because infections caused by multi-resistant staphylococci are more severe, chronic or latent, which increases the duration of treatment.

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“…), колонизирующий изолят способен вызвать инфекционный процесс, т.е. стать клинически значимым [1,10,11,12,13].…”

Section: результаты и обсуждениеunclassified

Veterinary monitoring of antibiotic resistance as a tool of infectious safety

Makavchik

2023

jour

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Veterinary antibiotic resistance monitoring is a systematic, ongoing process of collecting, analyzing and reporting data on antimicrobial resistance. The information received within the framework of monitoring has practical application points, including the organization of therapeutic and preventive measures.For veterinary monitoring, it is fundamentally important to separate all isolated microorganisms into clinical and colonization isolates.Clinically significant isolates, i.e. isolates that are the cause of the infectious process, specific localization.Colonizing bacteria that are not the cause of infection at a specific location. It should be noted that under certain conditions (entrance gates of infection, weakened immune status, etc.), a colonizing isolate can cause an infectious process, i.e. become clinically significant.For the preparation of local protocols for antimicrobial therapy of animals and birds, clinically significant isolates are of the greatest value.It is necessary to develop methodological recommendations for laboratory control of antibiotic resistance of pathogens of bacterial diseases of different species of animals, birds, fish, bees and introduce them into the practice of veterinary laboratories.

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Associated resistance of Escherichia coli isolated from humans and animals to polymyxin and beta-lactam antibiotics

Cited by 11 publications

References 44 publications

Antibiotic resistance of microbiota of drinking milk sold in St.Petersburg and Leningrad region

Antibiotic resistance of microbiota of drinking milk sold in St.Petersburg and Leningrad region

Laboratory methods for monitoring resistance to methycillin of Staphylococcus of zoonotic origin

Veterinary monitoring of antibiotic resistance as a tool of infectious safety

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