Association analysis, genetic diversity and structure analysis of tobacco based on AFLP markers

Dadras, Ahmad Reza; Sabouri, Hossein; Mohammadi-Nejad, Ghasem; Sabouri, Atefeh; Shoai-Deylami, M.

doi:10.1007/s11033-014-3194-6

Cited by 32 publications

(14 citation statements)

References 31 publications

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“…During this study, 11 iPBS-retrotransposon markers resulted in an average of 0.33 PIC for 96 Turkish tobacco germplasms. Dadras et al (2014) obtained a mean PIC value of 0.26 using a panel of 50 tobacco genotypes with AFLP markers, which is lower than the value of 0.33 obtained in our study. Lu et al (2013) investigated 330 high-quality DArTseq markers from a panel of 121 tobacco accessions and they resulted in an average PIC value of 0.34, which is close to our resulted PIC value.…”

Section: Discussioncontrasting

confidence: 95%

Genetic diversity assessment in Nicotianatabacum L. with iPBS-retrotransposons

Yaldız¹,

Çamlıca²,

Nadeem³

et al. 2018

Turk J Agric For

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Knowledge of genetic diversity could be beneficial by contributing important information in the selection of breeding material. The objective of this study was to explore the genetic diversity and relationship in the Turkish tobacco germplasm with the iPBSretrotransposons marker that emerged as a universal method. A total of 90 landraces and 6 commercial cultivars collected from different geographical regions of Turkey were used in this study. Eleven most polymorphic iPBS-retrotransposons primers yielded a total of 119 scorable bands and 98 of these bands were found to be polymorphic (82.35%), with an average of 8.91 polymorphic fragments for each primer. The mean polymorphism information contents were found to be 0.33, ranging from 0.10 to 0.80. The unweighted pair group method with arithmetic mean revealed that the genotypes belonging to the same geographical regions were often present in the same group and very close to each other. Based on the Bayesian clustering model, the genetic structure of Turkish tobacco germplasm was divided into 2 main groups. This is first study to explore the genetic diversity of Turkish tobacco by the iPBS-retrotransposons and we believe that in future the results of this study will serve as a foundation for the development of new and improved tobacco varieties in Turkey and the rest of world.

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Section: Discussioncontrasting

confidence: 95%

Genetic diversity assessment in Nicotianatabacum L. with iPBS-retrotransposons

Yaldız¹,

Çamlıca²,

Nadeem³

et al. 2018

Turk J Agric For

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“…However total soluble sugars per plant was negatively significantly correlated with nicotine percentage as total nicotine alkaloids and chlorides revealing the possibility to improve tobacco quality with low nicotine and chlorides using current population. The observed positive correlation of total soluble sugars with various traits was supported by earlier workers (Maleki et al, 2011;Dadras et al, 2014). This indicates the relative utility of all these traits for selection with respect to sugar content and low nicotine.…”

Section: Methodssupporting

confidence: 78%

“…Thus, there is sufficient chance for improvement of these traits and selection based on the phenotypic performance will be reliable and effective from this MAGIC F2 population. Furthermore, moderate to high heritability, GCV and GA% in a mean could be explained by additive gene action and their improvement could be achieved through mass selection in tobacco (Dobhal, 1987;Prasanna and Rao, 1989;Shah et al, 2008;Dadras et al, 2014) in other crops (Ogunbayo et al, 2014;Khatun et al, 2015;Hari et al, 2017).…”

Section: Methodsmentioning

confidence: 99%

“…Tobacco leaf is not only used for making cigarettes but also used both in traditional and concurrent medicine in treating insect bites, cuts and tumours (Dadras et al, 2014). More importantly, tobacco is an attractive green bioreactor proved to produce plant made vaccines, enzymes, immune modulatory molecules such as 2013 season and selected 3 to 4 morphotypes and intercrossed again to produce roots of eight way cross hybrids.…”

Section: Introductionmentioning

confidence: 99%

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Genetic Analysis and Trait Association in Multi-parent Advanced Generation Inter-Cross (MAGIC) F2 Population of Tobacco (Nicotiana tabacum L.)

Dhavala

Papidandla²,

Ullagaddi³

et al. 2017

Not Sci Biol

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Genetic variability studies offers basic information related to genetic properties of the population based on which breeding methods could be formulated for further improvement of the crop. The estimates of heritability, coefficients of variability and genetic advance was calculated in a multi-parent advanced generation inter-cross (MAGIC) F 2 segregating populations for 10 traits including nicotine percentage, total soluble sugars and yield contributing traits during 2015 seasons at Northern Light Soils (NLS). The estimates of phenotypic coefficients of variation (PCV) were high and moderate for plant height (18.16%), number of leaves (15.96%), leaf area index (27.35%), internodal length (21.01%), stem diameter (16.73%), grade index (13.86%), nicotine percentage (19.87%), total soluble sugars (39.81%), chlorides (23.35%) and leaf yield (13.27%) in MAGIC-F 2 segregating populations at NLS 2015 season. High heritability coupled with high and moderate genetic advance was observed for all the traits studied. Correlation studies revealed that total soluble sugars was positively and significantly correlated with leaf area index, stem diameter, grade index and leaf yield. However total soluble sugars was negatively correlated with nicotine as total nicotine alkaloid and with chlorides percentage. Path coefficient analysis revealed that total percentage of sugars could be improved through selection of grade index, stem diameter, leaf area index and cured leaf yield which were positively correlated with total soluble sugar percentage. The current study also revealed that the MAGIC F 2 population could be used for development high sugar lines with moderate or low levels of nicotine and chlorides in tobacco.

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“…Such a demonstration has been represented by some ISSR or SSR association studies on quince (Cydonia oblonga), white savory (Satureja mutica), Coffea canephora and Calophyllum inophyllum, respectively (Ganopoulos et al, 2011;Pawar et al, 2011;Khadivi-Khub et al, 2014;Achar et al, 2015). Based on amplified fragment length polymorphism (AFLP)-PCR, Dadras et al (2014) successfully identified a set of AFLP markers that closely linked to seven growth traits in tobacco. Pereira da Costa et al (2014) reported applying SRAP to identify the DNA loci linked to tomato fruit quality traits.…”

Section: Discussionmentioning

confidence: 99%

Association of SRAP markers with juvenile wood basic density and growth traits in Cunninghamia lanceolata (Lamb.) Hook

Hu¹,

Su²,

Wu³

et al. 2018

Dendrobiology

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Application of sequence-related amplified polymorphism (SRAP) markers to unravel variations and relationships with biological and morphological traits has been reported in a variety of plant species, and their potential for breeding has also been highlighted. (1) Assess the diversity level of a Cunninghamia lanceolata (Chinese fir) genetic panel based on phenotypic traits and SRAP markers, (2) identify SRAP loci linked to juvenile wood basic density (JWBD) and growth traits, and (3) address the overlap of the trait-associated SRAP markers during the juvenile and mature stages of this species. A total of 227 Chinese fir genotypes were subjected to phenotype, SRAP genotyping, and marker-trait association analyses. A total of 564 unambiguous SRAP bands and 558 polymorphic loci were identified from the genotypes. The overall percentage of polymorphic bands, polymorphism information content, Nei's gene diversity, and Shannon's Information Index were 98.9%, 0.2576, 0.3196 and 0.4838, respectively. An analysis of molecular variance further demonstrated that the genotypes varied significantly at SRAP polymorphisms (p < 0.01). A wide genetic distance span from 0.0531 to 0.9097 was also observed; most (94.9%) fell within the range of 0.3000-0.6999. An association analysis based on general linear model (GLM) and mixed linear model (MLM) unraveled 21, 26, 25, and 19 marker-trait associations for JWBD, height (H), diameter at breast height (DBH, 1.3 m) and stem volume (V), respectively. These marker-trait associations corresponded to 64 different SRAP markers; 46 of these were linked to only one trait, while the other 18 markers appeared to be associated with more than one trait but limited to growth traits. Overall, the SRAP markers represented R 2 (percentage of the phenotypic variation explained by marker) values of 1.7-9.2% for the GLM and 1.7-5.6% for the MLM. Strikingly, the significant trait-associated marker list seemed to be rather different from that of the previous study performed on mature traits (WBD, H, DBH and V), except for overlap of two markers. This study demonstrated an association of SRAP markers with JWBD and growth traits in Chinese fir. The results further our understanding of the genetic basis of the Chinese fir WBD and growth traits at the juvenile stage.

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Association analysis, genetic diversity and structure analysis of tobacco based on AFLP markers

Cited by 32 publications

References 31 publications

Genetic diversity assessment in Nicotianatabacum L. with iPBS-retrotransposons

Genetic diversity assessment in Nicotianatabacum L. with iPBS-retrotransposons

Genetic Analysis and Trait Association in Multi-parent Advanced Generation Inter-Cross (MAGIC) F2 Population of Tobacco (Nicotiana tabacum L.)

Association of SRAP markers with juvenile wood basic density and growth traits in Cunninghamia lanceolata (Lamb.) Hook

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