Association analysis of the ovine <i>KAP6-1</i> gene and wool traits in Barki sheep

Sallam, Ahmed M.; Gad-Allah, A. A.; Al-Bitar, Essam Mohamed

doi:10.1080/10495398.2020.1749064

Cited by 7 publications

(8 citation statements)

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“…This is consistent with the high frequency of the B allele in the studied population, which indicates that breeders may tend to select for it as a desirable allele to increase GFW (Asif et al, 2017). Likewise, it means that selection for the B allele at an early age may be worth it to increase wool production (Sallam et al, 2021). Conversely, wool produced from AA carriers had lower KS (0.45 ± 1.87 versus 1.98±1.88, P = 0.01) and increased the whiteness of the wool (+0.40 ± 1.73 versus −0.62 ± 1.74) compared to other genotypes.…”

Section: Discussionsupporting

confidence: 86%

“…Accordingly, it is hard to compare find- ings of this study with results of other reports. Nevertheless, variants in a different gene from the same family (i.e., KAP6-1) were associated with GCG and SST in Barki sheep (Sallam et al, 2021). Results of this study suggest that genetic markers may significantly influence subjective wool traits as well as objective measurements and should be considered.…”

Section: Discussionmentioning

confidence: 78%

“…This result is consistent with other findings on KAP family genes (Li et al, 2017;Tao et al, 2017;Farag et al, 2018). Conversely, higher (Farag et al, 2018;Ullah et al, 2020aUllah et al, , 2021 and lower (Ullah et al, 2020b;Sallam et al, 2021) numbers of alleles were identified in other KAP genes and different sheep populations. KRTAP6-5 was the most polymorphic gene in this family, which was identified in goats with 12 variants (Li et al, 2021).…”

Section: Discussionmentioning

confidence: 98%

“…Therefore, SNPs became the most common genetic marker used in selection and evaluation programs in livestock (Dominik et al, 2021). Conversely, scarce information is available about the genetic background of wool traits as few genes (e.g., KAP1.1, KAP1.3 and KAP6) were identified for wool traits in Egyptian sheep (Farag et al, 2018;Sallam et al, 2021).…”

Section: Introductionmentioning

confidence: 99%

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Genetic variation in the ovine KAP22-1 gene and its effect on wool traits in Egyptian sheep

2022

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Abstract. The objective of this study was to investigate the genetic polymorphisms in the keratin-associated protein (KAP22-1) gene in Barki (n=206), Rahmani (n=28) and Ossimi (n=28) as the three major sheep breeds in Egypt. Subsequently, the detected variants were correlated with important wool traits. The traits included greasy fleece weight (GFW, g), staple length (SL, cm), prickle factor (PF, %), medullated fiber (MF, %), fiber diameter (FD, µm), crimp percentage (CR, %) and the standard deviation of FD (SDfd, µm), as well as the subjectively assessed traits of kemp score (KS), handle grade (HG), greasy color grade (GCG), bulk grade (BG), luster grade (LG) and staple structure (SST). Animals were genotyped by polymerase chain reaction (PCR) – single strand conformation polymorphism (SSCP). Five SSCP banding patterns representing three different nucleotide variants (A, B and C) were detected. DNA sequencing confirmed three single nucleotide polymorphisms (SNPs). Animal age significantly affected GFW (P=0.007), SDfd (P=0.006), SL (P=0.002), CR (P=0.006), KS (P=0.001), LG (P=0.006) and SST (P=0.013). Likewise, the breed had a significant effect on all studied traits except HG and BG, which was not significant. Results showed significant associations between the KAP22-1 variants and CR (P=0.01), SL (P=0.012), KS (P<0.001) and GCG (P=0.01). Interestingly, animals with BB genotypes tended to produce more wool yield (1163.63±65.91 g) with high SL (8.38±0.20 cm), CR (8.38±0.21 %) and KS (1.98±1.88). Results of this study strongly recommend the KAP22-1 gene as a candidate gene for wool production traits in Egyptian sheep, with new useful insights into the visually assessed wool traits. The identified genetic markers may be incorporated into breeding strategies and genetic improvement programs of wool traits in Egyptian sheep.

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Section: Discussionsupporting

confidence: 86%

Section: Discussionmentioning

confidence: 78%

Section: Discussionmentioning

confidence: 98%

Section: Introductionmentioning

confidence: 99%

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Genetic variation in the ovine KAP22-1 gene and its effect on wool traits in Egyptian sheep

2022

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“…This involves screening candidate genes, which may be biologically related to the desired trait, for putative mutations, and consequently correlating these results with accurate phenotypes of a group of individuals [18,19]. The identified genetic markers may be useful in selection and breeding programs in livestock [20]. Variations in the non-coding regions of the MSTN gene have been found to relate to muscle growth and meat quality traits in sheep [21,22], which can be attributed to their effects on the regulatory elements of the gene itself [23].…”

Section: Introductionmentioning

confidence: 99%

Genetic variations in the Myostatin gene affecting growth traits in sheep

et al. 2021

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Background and Aim: Sheep productivity in developing countries is crucial, as this animal is an essential source of meat and wool. Myostatin (MSTN) plays an important role in the regulation of muscle mass through the regulation of muscle growth, differentiation, and regeneration. The present study sought to investigate genetic variation in the first intron of the MSTN gene and the association of variants with growth traits in major sheep breeds in Egypt (Barki, Ossimi, and Rahmani) and Saudi Arabia (Najdi) using polymerase chain reaction (PCR) and sequencing. Materials and Methods: Blood samples were collected, and DNA was extracted from 75 animals. A 386 bp fragment in the first intron of the MSTN gene was amplified using PCR. Polymorphic sites were detected using direct sequencing and then correlated with growth traits using a general linear model. Results: Sequence analysis of the first intron of MSTN gene identified six single-nucleotide polymorphisms (SNPs) in the studied breeds. Four mutual SNPs were determined: c.18 G>T, c.241 T>C, c.243 G>A, and c.259 G>T. In addition, two SNPs c.159 A>T and c.173 T>G were monomorphic (AA and TT, respectively) in the Ossimi, Rahmani, and Najdi breeds and polymorphic in the Barki breed. The association analysis revealed that the c.18 G>T and c.241 C>T significantly associated (p<0.05) with birth weight and average daily weight gain, respectively. Conclusion: Our results strongly support MSTN as a candidate gene for marker-assisted selection in sheep breeding programs. Furthermore, the identified variants may be considered as putative markers to improve growth traits in sheep.

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Applications of Omics Technologies in Livestock Production, Improvement and Sustainability

Maru,

Kumar

2024

Sustainable Agriculture Reviews

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Association analysis of the ovine KAP6-1 gene and wool traits in Barki sheep

Cited by 7 publications

References 30 publications

Genetic variation in the ovine KAP22-1 gene and its effect on wool traits in Egyptian sheep

Genetic variation in the ovine KAP22-1 gene and its effect on wool traits in Egyptian sheep

Genetic variations in the Myostatin gene affecting growth traits in sheep

Applications of Omics Technologies in Livestock Production, Improvement and Sustainability

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