Association and Coexpression of Fatty‐Acid‐Binding Protein and Glycoprotein CD36 in the Bovine Mammary Gland

Spitsberg, Vitaly L.; Matitashvili, Elvina; Gorewit, R.C.

doi:10.1111/j.1432-1033.1995.0872g.x

Cited by 29 publications

(24 citation statements)

References 30 publications

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“…Compared to monogastric animals, ruminants have different digestive and absorptive mechanisms involving rumen fermentation. In ruminants, expression of FABPs is confirmed within the liver, heart, mammary gland and muscle (Haunerland et al 1984;Glatz et al 1985;Moore et al 1991;Spitsberg et al 1995). LCFAs play an important role in growth of calves as energy metabolism, steroid and cholesterol production, and LCFAs are also needed for milk fat secretion in lactating cows.…”

Section: Introductionmentioning

confidence: 99%

Fatty acid‐binding protein expression in the gastrointestinal tract of calves and cows

Hayashi

Maruyama

Fukuoka

et al. 2012

Animal Science Journal

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Fatty acid-binding protein (FABP) has high affinity for long-chain fatty acids and appears to participate in the metabolism and intracellular transport of lipids. Liver- and intestinal-type FABP (L-FABP and I-FABP, respectively) are expressed in the small intestine. However, in the gastrointestinal tract of ruminants, expression and localization of FABPs are unknown. In this study, we investigated the expression of I-FABP and L-FABP in the gastrointestinal tract of cattle. I- and L-FABP had higher messenger RNA (mRNA) and protein expression levels in the duodenum and jejunum relatively to other gastrointestinal regions in both calves and cows. Furthermore, L-FABP mRNA and protein expression were high in the colon. Both these protein types were confirmed to be in the cytosol of jejunal epithelial cells, where they were found in the villi rather than in the crypts. We concluded that duodenal and jejunal FABPs might be involved in the metabolism of fatty acids mainly in epithelial cells in cattle.

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Section: Introductionmentioning

confidence: 99%

Fatty acid‐binding protein expression in the gastrointestinal tract of calves and cows

Hayashi

Maruyama

Fukuoka

et al. 2012

Animal Science Journal

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“…Two sites in the CD36 gene were correlated with collocated fat concentration and milk yield QTL, with lower correlations also observed for the lactose yield and protein concentration phenotypes. This gene encodes a glycoprotein that has been implicated in fatty acid transport (Ibrahimi and Abumrad, 2002), and in mammary gland cell proliferation and involution (Spitsberg et al, 1995). The HOOK3 gene contained three sites with edQTL that were strongly correlated with QTL for lactose concentration or fat yield.…”

Section: Rna Edited Genes and Qtlmentioning

confidence: 99%

Widespread cis-regulation of RNA-editing in a large mammal

Lopdell

Couldrey

Tiplady

et al. 2018

Preprint

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Post-transcriptional RNA editing may regulate transcript expression and diversity in cells, with potential impacts on various aspects of physiology and environmental adaptation. A small number of recent genome-wide studies in Drosophila, mouse, and human have shown that RNA editing can be genetically modulated, highlighting loci that quantitatively impact editing of transcripts. The potential gene expression and physiological consequences of these RNA editing quantitative trait loci (edQTL), however, are almost entirely unknown. Here, we present analyses of RNA editing in a large domestic mammal (Bos taurus), where we use whole genome and high depth RNA sequencing to discover, characterise, and conduct genetic mapping studies of novel transcript edits. Using a discovery population of nine deeply-sequenced cows, we identify 2,001 edit sites in the mammary transcriptome, the majority of which are adenosine to inosine edits (97.4%). Most sites are predicted to reside in double-stranded secondary structures (85.7%), and quantification of the rates of editing in an additional 355 cows reveals editing is negatively correlated with gene expression in the majority of cases. Genetic analyses of RNA editing and gene expression highlights 67 cis-regulated edQTL, of which seven appear to co-segregate with expression QTL effects. Trait association analyses in a separate population of 9,988 lactating cows also shows nine of the cis-edQTL coincide with at least one co-segregating lactation QTL. Together, these results enhance our understanding of RNA editing dynamics in mammals, and suggest mechanistic links by which loci may impact phenotype through RNA-editing mediated processes.

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“…Two sites in the CD36 gene were correlated with colocated fat concentration and milk yield QTL, with lower correlations also observed for the lactose yield and protein concentration phenotypes. This gene encodes a component of the milk fat globule membrane, comprising a glycoprotein that is implicated in fatty acid transport (Ibrahimi and Abumrad 2002), and in mammary gland cell proliferation and involution (Spitsberg et al 1995). The HOOK3 gene contained three sites with edQTL that were strongly correlated with QTL for lactose concentration or fat yield.…”

Section: Rna Edited Genes and Qtlmentioning

confidence: 99%

Widespread cis-regulation of RNA editing in a large mammal

Lopdell

Hawkins

Couldrey

et al. 2018

RNA

View full text Add to dashboard Cite

Post-transcriptional RNA editing may regulate transcript expression and diversity in cells, with potential impacts on various aspects of physiology and environmental adaptation. A small number of recent genome-wide studies in Drosophila, mouse, and human have shown that RNA editing can be genetically modulated, highlighting loci that quantitatively impact editing of transcripts. The potential gene expression and physiological consequences of these RNA-editing quantitative trait loci (edQTL), however, are almost entirely unknown. Here, we present analyses of RNA editing in a large domestic mammal (Bos taurus), where we use whole-genome and high-depth RNA sequencing to discover, characterize, and conduct genetic mapping studies of novel transcript edits. Using a discovery population of nine deeply sequenced cows, we identify 2413 edit sites in the mammary transcriptome, the majority of which are adenosine to inosine edits (98.6%). Most sites are predicted to reside in double-stranded secondary structures (85.1%), and quantification of the rates of editing in an additional 355 cows reveals editing is negatively correlated with gene expression in the majority of cases. Genetic analyses of RNA editing and gene expression highlight 152 cis-regulated edQTL, of which 15 appear to cosegregate with expression QTL effects. Trait association analyses in a separate population of 9989 lactating cows also shows 12 of the cis-edQTL coincide with at least one cosegregating lactation QTL. Together, these results enhance our understanding of RNA-editing dynamics in mammals, and suggest mechanistic links by which loci may impact phenotype through RNA editing mediated processes.

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Association and Coexpression of Fatty‐Acid‐Binding Protein and Glycoprotein CD36 in the Bovine Mammary Gland

Cited by 29 publications

References 30 publications

Fatty acid‐binding protein expression in the gastrointestinal tract of calves and cows

Fatty acid‐binding protein expression in the gastrointestinal tract of calves and cows

Widespread cis-regulation of RNA-editing in a large mammal

Widespread cis-regulation of RNA editing in a large mammal

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