Association and Virulence Gene Expression Vary among Serotype III Group B Streptococcus Isolates following Exposure to Decidual and Lung Epithelial Cells

Korir, Michelle L.; Knupp, David; LeMerise, Kathryn; Boldenow, Erica; Loch‐Caruso, Rita; Aronoff, David M.; Manning, Shannon D.

doi:10.1128/iai.02181-14

Cited by 26 publications

(36 citation statements)

References 47 publications

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“…Korir et al . utilized decidualized human endometrial stromal cells to study attachment and invasion by various strains of GBS [44], finding strain-dependent differences and a proclivity for invasive GBS strains to be the most capable at adhering and invading [44]. …”

Section: Methodsmentioning

confidence: 99%

Current concepts in maternal-fetal immunology: Recognition and response to microbial pathogens by decidual stromal cells

Anders

Gaddy

Doster

et al. 2017

Am J Reprod Immunol

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Chorioamnionitis is an acute inflammation of the gestational (extraplacental) membranes, most commonly caused by ascending microbial infection. It is associated with adverse neonatal outcomes including preterm birth, neonatal sepsis and cerebral palsy. The decidua is the outermost layer of the gestational membranes and is likely an important initial site of contact with microbes during ascending infection. However, little is known about how decidual stromal cells (DSCs) respond to microbial threat. Defining the contributions of individual cell types to the complex medley of inflammatory signals during chorioamnionitis could lead to improved interventions aimed at halting this disease. We review available published data supporting the role for DSCs in responding to microbial infection, with a special focus on their expression of pattern recognition receptors and evidence of their responsiveness to pathogen sensing. While DSCs likely play an important role in sensing and responding to infection during the pathogenesis of chorioamnionitis, important knowledge gaps and areas for future research are highlighted.

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Section: Methodsmentioning

confidence: 99%

Current concepts in maternal-fetal immunology: Recognition and response to microbial pathogens by decidual stromal cells

Anders

Gaddy

Doster

et al. 2017

Am J Reprod Immunol

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“…With the exception of NEM316 41 and COH1, 42 invasive strains were originally recovered from neonatal blood or cerebral spinal fluid in a prior study, 43 and colonizing strains were recovered via vaginal/rectal swabs from women during and after pregnancy. 44 The ST-17 strain (GB00112) was examined in our prior studies, 45,46 and all strains were previously characterized by capsular genotyping and MLST as described. 8,47 Cell culture…”

Section: Bacterial Culturementioning

confidence: 99%

“…45 RNA samples were collected from bacteria cultured in medium by adding samples to 2 volumes of RNAprotect Bacteria Reagent (Qiagen). RNA samples were collected from intracellular bacteria during the survival assay described above by washing the wells twice with PBS then adding 1ml RNAprotect Bacteria Reagent.…”

Section: Rna Isolation and Rt-pcrmentioning

confidence: 99%

Differing mechanisms of surviving phagosomal stress among group BStreptococcusstrains of varying genotypes

et al. 2016

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Group B Streptococcus (GBS), a leading cause of neonatal sepsis and meningitis, asymptomatically colonizes up to 30% of women and can persistently colonize even after antibiotic treatment. Previous studies have shown that GBS resides inside macrophages, but the mechanism by which it survives remains unknown. Here, we examined the ability of 4 GBS strains to survive inside macrophages and then focused on 2 strains belonging to sequence type (ST)-17 and ST-12, to examine persistence in the presence of antibiotics. A multiple stress medium was also developed using several stressors found in the phagosome to assess the ability of 30 GBS strains to withstand phagosomal stress. The ST-17 strain was more readily phagocytosed and survived intracellularly longer than the ST-12 strain, but the ST-12 strain was tolerant to ampicillin unlike the ST-17 strain. Exposure to sub-inhibitory concentrations of ampicillin and erythromycin increased the level of phagocytosis of the ST-17 strain, but had no effect on the ST-12 strain. In addition, blocking acidification of the phagosome decreased the survival of the ST-17 strain indicating a pHdependent survival mechanism for the ST-17 strain. Congruent with the macrophage experiments, the ST-17 strain had a higher survival rate in the multiple stress medium than the ST-12 strain, and overall, serotype III isolates survived significantly better than other serotypes. These results indicate that diverse GBS strains may use differing mechanisms to persist and that serotype III strains are better able to survive specific stressors inside the phagosome relative to other serotypes.

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“…Association assays were performed as described previously [ 28 , 32 ]. Briefly, monolayers of decidual cells were washed thrice with 1x PBS before infection with bacterial cultures.…”

Section: Methodsmentioning

confidence: 99%

“…Monolayers of dT-HESCs were cultured in 24-well plates and cells were incubated with lactobacilli (MOI = 10) using a protocol for GBS described by Korir, et al [ 32 ]. After incubation for four hours, cells were washed twice with 1x PBS, treated with 4μM ethidium homodimer 1 (Molecular Probes, Eugene, OR), suspended in 1x PBS, and incubated at room temperature for 30 minutes without light.…”

Section: Methodsmentioning

confidence: 99%

Lactobacillus strains vary in their ability to interact with human endometrial stromal cells

Shiroda

Manning

2020

PLoS ONE

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The placental membranes that surround the fetus during pregnancy were suggested to contain a low abundance microbiota. Specifically, abundance of Lactobacillus , a probiotic and dominant member of the microbiome of the lower reproductive tract, has been shown to correlate with healthy, term pregnancies. We therefore sought to assess the interactions between four different Lactobacillus strains with immortalized decidualized endometrial cells (dT-HESCs), which were used as a model to represent the outermost layer of the placental membranes. Notably, we demonstrated that all four Lactobacillus strains could associate with dT-HESCs in vitro . L . crispatus was significantly more successful (p < 0.00005), with 10.6% of bacteria attaching to the host cells compared to an average of 0.8% for the remaining three strains. The four strains also varied in their ability to form biofilms. Dependent on media type, L . reuteri 6475 formed the strongest biofilms in vitro . To examine the impact on immune responses, levels of total and phosphorylated protein p38, a member of the Mitogen Activated Protein Kinase (MAPK) pathway, were examined following Lactobacillus association with dT-HESCs. Total levels of p38 were reduced to an average of 44% that of the cells without Lactobacillus (p < 0.05). While a trend towards a reduction in phosphorylated p38 was observed, this difference was not significant (p > 0.05). In addition, association with Lactobacillus did not result in increased host cell death. Collectively, these data suggest that varying types of Lactobacillus can attach to the outermost cells of the placental membranes and that these interactions do not contribute to inflammatory responses or host cell death. To our knowledge this is the first in vitro study to support the ability of Lactobacillus to interact with placental cells, which is important when considering its use as a potential probiotic within the reproductive tract.

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Association and Virulence Gene Expression Vary among Serotype III Group B Streptococcus Isolates following Exposure to Decidual and Lung Epithelial Cells

Cited by 26 publications

References 47 publications

Current concepts in maternal-fetal immunology: Recognition and response to microbial pathogens by decidual stromal cells

Current concepts in maternal-fetal immunology: Recognition and response to microbial pathogens by decidual stromal cells

Differing mechanisms of surviving phagosomal stress among group BStreptococcusstrains of varying genotypes

Lactobacillus strains vary in their ability to interact with human endometrial stromal cells

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