Association between adipose tissue expression and serum levels of leptin and adiponectin in women with polycystic ovary syndrome

Abstract

ABSTRACT. We reviewed emerging evidence linking serum levels and adipose tissue expression of leptin and adiponectin in women with polycystic ovary syndrome (PCOS). Previous data obtained by our group from a sample of overweight/obese PCOS women and a control sample of normal weight controls, both stratified by BMI, were reanalyzed. Circulating levels of leptin and adiponectin were determined by commercially available enzyme-linked immunosorbent assays. Adipose tissue total RNA was reserve-transcripted into complementary DNA samples, which were used as templates for quantitative real-time PCR amplification. Positive correlations were found between serum and mRNA levels for both leptin (r = 0.321; P = 0.005) and adiponectin (r = 0.266; P = 0.024). Deter-©FUNPEC-RP www.funpecrp.com.br Genetics and Molecular Research 12 (4): 4292-4296 (2013) Adipose tissue expression in PCOS 4293 mination of leptin and adiponectin serum levels could serve as an indirect method to assess adipocyte production, since leptin and adiponectin are predominantly produced by subcutaneous adipocytes in women.

Adipose tissue dysfunction, adipokines, and low-grade chronic inflammation in polycystic ovary syndrome

“…As previously reported by us, leptin gene expression was higher in SC fat of overweight/ obese PCOS in comparison with normal-weight controls. In contrast, adiponectin gene expression did not differ between obese and non-obese subgroups (Lecke et al 2011a(Lecke et al , 2013b. These data are in agreement with other reports regarding PCOS and control populations (Wang et al 2012).…”

“…While our group and other authors found similar circulating adiponectin levels in BMI-matched PCOS and control women (Lecke et al 2011a, 2013b, Pepene 2012, Tan et al 2013, other studies have reported lower adiponectin levels in PCOS women in comparison with healthy controls, independently of BMI: a meta-analysis including more than 3500 subjects (PCOS women and healthy BMI-matched controls) of various ages with different levels of total testosterone and insulin found that adiponectin was significantly lower in PCOS (Li et al 2014). Reduced adiponectin levels appear to play a role in promoting insulin resistance increasing triglycerides and small and dense LDL particles (Tan et al 2006a).…”

“…We have previously reported that leptin serum levels are higher in overweight/obese PCOS women and also in BMI-matched controls than in normal-weight PCOS and controls (Spritzer et al 2005, Lecke et al 2011a, 2013b. In fact, most published studies about circulating leptin levels in PCOS and controls, including adolescents and pre-and postmenopausal women, stratified or not by BMI, have demonstrated that circulating leptin levels are associated with the amount of body fat, being significantly increased in overweight/obese subgroups, independently of the presence of PCOS (Plati et al 2010, Tan et al 2013.…”

Validity of adiponectin-to-leptin and adiponectin-to-resistin ratios as predictors of polycystic ovary syndrome

“…The association between altered adiponectin levels and PCOS remains controversial, with some studies reporting comparable adiponectin levels in BMI-matched PCOS and control women (11,12,17), and others (8,9,11,16), including two meta-analyses (18,19), showing that women with PCOS have lower levels of adiponectin independent of body mass index (BMI).…”

“…Also, PCOS is associated with obesity, central adiposity, and dyslipidemia (3)(4)(5), and an increased risk of type 2 diabetes (T2DM) (1,5,6), which have been linked to insulin resistance (IR) (7,8). Insofar as adipose tissue secretes bioactive cytokines and adipokines, including adiponectin, leptin, and resistin (9), mounting evidence implicates dysregulated adipokine expression with the onset of obesity-related pathologies including PCOS (9)(10)(11)(12).…”