Association Between BoLA-DRB3.2 Polymorphism and Bovine Papillomavirus Infection for Bladder Tumor Risk in Podolica Cattle

Abstract

Blood samples from 260 unrelated cattle (132 animals affected by papillomavirus-associated bladder tumors and 128 healthy) were genotyped using the classic polymerase chain reaction/restriction fragment length polymorphism method to screen MHC class II bovine leukocyte antigen-DRB3. 2 polymorphism. The DRB3*22 allele was significantly (p ≤ 0.01) detected in healthy cattle, thus appearing to have a negative association (protective effect) with virus infection of the urinary bladder known to represent a bladder tumor risk for cattle living free at pasture. Considering the two sequence alleles identified in animals carrying DRB3*22, DRB3*011:01 allele from samples of animals harboring the unexpressed bovine papillomaviruses (BPV)-2 E5 gene was characterized by amino acid residues believed to have a protective effect against BPV infection such as arginine at position 71 (R71) in pocket 4, histidine at position 11 (H11) in pocket 6, and both glutamine at position 9 (Q9) and serine at position 57 (S57) in pocket 9 of the antigen-binding groove. The DRB3*011:02v allele from affected animals was characterized by amino acids believed to be susceptibility residues such as lysine (K71), tyrosine (Y11), glutamic acid (E9), and aspartic acid (D57) in these pockets. These results suggest that animals harboring the DRB3*011:01 allele may have a lower risk of BPV infection and, consequently, a reduced risk of bladder tumors.

BoLA-DRB3 Polymorphism Controls Proviral Load and Infectivity of Bovine Leukemia Virus (BLV) in Milk

“…Previous studies have shown that BoLA-DRB3 polymorphism in BLV-infected cattle is related to blood proviral load (PVL), infectivity, lymphoma, and in utero infection of calves [18,[23][24][25][26][27][28][29]. In addition to BLV, BoLA-DRB3 polymorphism is associated with infectious diseases such as bovine clinical mastitis, papillomavirus infection, herpes virus type 1 infection, and foot-and-mouth disease [30][31][32][33]. Therefore, the BoLA-DRB3 polymorphism is considered a useful gene in immunology.…”

Pteridium spp. and Bovine Papillomavirus: Partners in Cancer

“…More recently, BPV-13 (86,130) and BPV-14 (131) have also been found in bovine urinary bladder tumors. Interestingly, recent data indicate that specific major histocompatibility complex class II alleles may protect against BPV infection and reduce the risk of bladder cancer in cattle (132). The BPV-1 E5 oncoprotein shows transforming properties in vitro and is able to induce cytoskeletal remodeling of murine and bovine cells (133).…”

Association of <i>BoLA-DRB3</i> with bovine leukemia virus

“…The polymorphism in this region is therefore associated with the variability in the immune responsiveness in different individuals to particular infectious diseases and economic traits such as reproduction 22) , colostrum and milk microbiota 23) , milk quality and production rate 5) . For infection disease, BoLA-DRB3 relates with mastitis 24,25) , tick-borne disease 26,27) , foot and mouth disease (FMD) 28) , bovine herpesvirus 1 (BoHV-1) 29) , bovine papillomavirus-induced bladder cancer 30) , but not relates with the bovine tuberculosis (bTB) susceptibility 31) . The association studies of BoLA-DRB3 and economic traits and disease are summarized in Table 2.…”