Association between CD14 polymorphisms and serum soluble CD14 levels: Effect of atopy and endotoxin inhalation

LeVan, Tricia D.; Michel, Olivier; Dentener, Mieke A.; Thorn, Jörgen; Vertongen, Françoise; Beijer, Lena; Martínez, Fernando D.

doi:10.1016/j.jaci.2007.08.050

Cited by 46 publications

(57 citation statements)

References 46 publications

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“…In our study, atopic girls showed significantly lower serum sCD14 levels than atopic boys. This is in accordance with the findings of LeVan et al (19) that male subjects had higher sCD14 levels than female subjects. These results may indicate that atopy affects innate immunity differently (25-75 %), and the whisker corresponds to 1.5 times the interquartile range in women and men, suppressing CD14 activity more in women than in men.…”

Section: Discussionsupporting

confidence: 93%

“…Beside methodological differences between the studies, one reason for the inconsistencies may be the role of disease status on serum sCD14, because acute inflammation and acute atopic asthma have been associated with a higher production of CD14 (20,(23)(24)(25). In contrast, mild atopic manifestations may be associated with milder changes in sCD14 levels, as was observed in the our and several other studies (6,11,19). In our study, atopics presented no severe clinical features of atopic disease.…”

Section: Discussionsupporting

confidence: 73%

“…This is why we believe that the observed inconsistencies between earlier reports could be explained by the association between the acuteness of atopic diseases and CD14 expression (14,23). Other reasons for the inconsistencies may be the influence of microbial and allergen load interacting with CD14 as well as the CD14 gene polymorphisms (19,26).…”

Section: Discussionmentioning

confidence: 86%

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Soluble CD14 and total IgE in the serum of atopic and nonatopic adolescents in relation to environmental factors: a pilot study / Topivi receptor CD14 i ukupni IgE u serumu mladih atopičara odrasle dobi u ovisnosti o čimbenicima iz okoliša: pilot istraživanje

Prester

Varnai

Macan

2015

Archives of Industrial Hygiene and Toxicology

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The CD14 receptor is expressed on the surfaces of monocytes, macrophages, and, to a lesser extent, of activated granulocytes and B lymphocytes (mCD14). It also exists in soluble form in the serum (sCD14), in which it binds microbial compounds and indoor allergens. The objectives of this study were to see whether serum sCD14 concentrations could be used as a marker of atopic disorders and to estimate the effects of environmental factors (tobacco smoke exposure, childhood residence in urban or rural areas, and having a pet) on sCD14 and IgE values. Mass fraction of sCD14 and total IgE were determined in the sera of atopic (N=53) and non-atopic (N=35) participants using the standard enzyme-linked immunosorbent assay. Exposure to pets and environmental tobacco smoke was estimated based on the International Study of Asthma and Allergies in Childhood questionnaire (ISAAC). Median sCD14 concentration was significantly lower in atopic than in non-atopic girls (3.49 vs. 3.83 μg mL -1 ; p<0.010). The number of smokers at home positively correlated with the sCD14 level in atopics, and urban atopics had significantly lower sCD14 levels than rural atopics (3.47 vs. 3.92 μg mL -1 , p=0.028). Median total IgE concentration was significantly lower in atopic pet owners than in atopics with no pets (161 vs. 252 kIU L -1 ; p=0.021). In conclusion, while sex and environmental factors might be involved in sCD14 expression, particularly in atopics, we found no correlation between sCD14 and total IgE concentrations. The usefulness of sCD14 as a marker of atopic disorders should be investigated further, particularly in relation to the severity of allergic disorders.KEY WORDS: allergy; atopy; environmental tobacco smoke; immunoglobulin E; sCD14; pets Prester Lj, et al. Soluble CD14 and total IgE in the serum of atopic and non-atopic adolescents in relation to environmental factors: a pilot study Arh Hig Rada Toksikol 2015;66:41-49

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Section: Discussionsupporting

confidence: 93%

Section: Discussionsupporting

confidence: 73%

Section: Discussionmentioning

confidence: 86%

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Soluble CD14 and total IgE in the serum of atopic and nonatopic adolescents in relation to environmental factors: a pilot study / Topivi receptor CD14 i ukupni IgE u serumu mladih atopičara odrasle dobi u ovisnosti o čimbenicima iz okoliša: pilot istraživanje

Prester

Varnai

Macan

2015

Archives of Industrial Hygiene and Toxicology

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“…Recently, a single-nucleotide polymorphism in the promoter region of CD14 has been studied in patients with allergy [26][27][28][29][30][31]. Early studies suggested that this variant, which affects the soluble CD14 level [27,28], was associated with more severe atopy [27,29,30].…”

Section: Discussionmentioning

confidence: 99%

“…Early studies suggested that this variant, which affects the soluble CD14 level [27,28], was associated with more severe atopy [27,29,30]. Although this finding was not uniformly replicated [31] and consideration of LPS exposure level is required to analyse the data [26], these findings indicated that interaction between LPS and CD14/TLR may be involved in the pathophysiology of some aspect of asthma.…”

Section: Discussionmentioning

confidence: 99%

Trans-Basement Membrane Migration of Eosinophils Induced By LPS-Stimulated Neutrophils from Human Peripheral Blood in Vitro

Nakagome

Nishihara

Kobayashi

et al. 2016

Journal of Allergy and Clinical Immunology

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In the airways of severe asthmatics, an increase of neutrophils and eosinophils is often observed despite high-dose corticosteroid therapy. We previously reported that interleukin-8-stimulated neutrophils induced trans-basement membrane migration (TBM) of eosinophils, suggesting the link between neutrophils and eosinophils. Concentrations of lipopolysaccharide (LPS) in the airway increase in severe asthma. As neutrophils express Toll-like receptor (TLR)4 and can release chemoattractants for eosinophils, we investigated whether LPS-stimulated neutrophils modify eosinophil TBM.Neutrophils and eosinophils were isolated from peripheral blood of healthy volunteers and severe asthmatics. Eosinophil TBM was examined using a modified Boyden's chamber technique. Eosinophils were added to the upper compartment, and neutrophils and LPS were added to the lower compartment. Migrated eosinophils were measured by eosinophil peroxidase assays.LPS-stimulated neutrophils induced eosinophil TBM (about 10-fold increase), although LPS or neutrophils alone did not. A leukotriene B 4 receptor antagonist, a platelet-activating factor receptor antagonist or an anti-TLR4 antibody decreased eosinophil TBM enhanced by LPS-stimulated neutrophils by almost half. Neutrophils from severe asthmatics induced eosinophil TBM and lower concentrations of LPS augmented neutrophil-induced eosinophil TBM.These results suggest that the combination of neutrophils and LPS leads eosinophils to accumulate in the airways, possibly involved the pathogenesis of severe asthma. @ERSpublications LPS-stimulated neutrophils induced eosinophil TBM, which may be involved in the pathogenesis of severe asthma

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CD14 is associated with biliary stricture formation

et al. 2016

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The pathogenesis of intrahepatic biliary stricture formation in patients with primary sclerosing cholangitis (PSC) or after liver transplantation (LTx) remains elusive. CD14 receptor signaling is a key mediator of the innate immune system; its common genetic variant is associated with alcoholic liver disease. PSC and LTx cohort patients and primary biliary cirrhosis (PBC) control patients were genotyped for the CD14 -260C>T (rs2569190) polymorphism, and genotypes were correlated with long-term clinical outcome. Biliary tissue, bile, and whole blood of PSC patients and healthy controls were screened for markers of the innate immune system and bacterial infection. In 121 PSC patients, the CD14 -260C>T genotype was associated with development of dominant bile duct strictures (P 5 0.02). In 365 LTx patients, TT carriers (4.1%) were protected against the formation of nonanastomotic biliary strictures versus CC/CT patients (12.6%; P 5 0.01). Chemokine ligand 8 (P 5 0.04) and chemokine receptor 6 (P 5 0.004) were up-regulated in biliary tissue of PSC patients with the TT versus the CC/CT genotype. Lipopolysaccharide whole-blood stimulation resulted in a significant change in interleukin (IL)-8 (P 5 0.05) and IL-12p40 levels (P 5 0.04) in healthy control subjects carrying the TT genotype. TT PSC patients were protected against Gram-negative bacterial biliary infection (TT: 0% vs. CC/CT: 22.5%; P 5 0.02). Serum-soluble CD14 levels correlated with the CD14 -260C>T genotype (P 5 0.02), representing an independent risk indicator of survival in PSC patients (hazard ratio, 0.40; 95% confidence interval, 0.19-0.86; P 50.01). Conclusions: The function of the innate immune response by CD14 is crucial during biliary infection and stricture formation. The benefits of CD14 signaling modification should be addressed in future studies. (HEPATOLOGY 2016;64:843-852)

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Association between CD14 polymorphisms and serum soluble CD14 levels: Effect of atopy and endotoxin inhalation

Cited by 46 publications

References 46 publications

Soluble CD14 and total IgE in the serum of atopic and nonatopic adolescents in relation to environmental factors: a pilot study / Topivi receptor CD14 i ukupni IgE u serumu mladih atopičara odrasle dobi u ovisnosti o čimbenicima iz okoliša: pilot istraživanje

Soluble CD14 and total IgE in the serum of atopic and nonatopic adolescents in relation to environmental factors: a pilot study / Topivi receptor CD14 i ukupni IgE u serumu mladih atopičara odrasle dobi u ovisnosti o čimbenicima iz okoliša: pilot istraživanje

Trans-Basement Membrane Migration of Eosinophils Induced By LPS-Stimulated Neutrophils from Human Peripheral Blood in Vitro

CD14 is associated with biliary stricture formation

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