Association between circulating microRNA-208a and severity of coronary heart disease

Zhang, Yao; Li, Haihong; Yang, Rui; Bian, Yang; Gao, Zhaoyu

doi:10.1080/00365513.2017.1328740

Cited by 37 publications

(16 citation statements)

References 23 publications

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“…The cDNA obtained was pre-amplified as described above for blood sample and amplified using the specific TaqMan micro-RNA assays (Life Technologies). 17 Primers for miR-19b were given as: forward, 5′-TGA TAA TTA GCA AGC AGG ATT A-3′ and reverse, 5′-ACC AAC ATT ACG CGG CAT CAT TA-3′. RNU6B was used as endogenous control.…”

Section: Quantitative Assessment Of Mirna-19bmentioning

confidence: 99%

Diagnostic value of circulating microRNA‐19b in heart failure

Zhang

Liu

et al. 2020

Eur J Clin Investigation

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Objective: For differentiating heart failure (HF) with preserved ejection fraction (HFpEF) from HF with reduced EF (HFrEF), N-terminal prohormone brain natriuretic peptide (NT-proBNP) is less accurate. Decreased expression of microRNA-19b (miR-19b) is associated with increased cardiac-fibrosis. We aim to evaluate the value of miR-19b in diagnosing HFrEF patients. Method: We included 200 HF patients and 100 healthy controls. Intergroup comparisons of miR-19b were made and correlation between miR-19b and NT-proBNP was analysed. Diagnostic values of NT-proBNP and miR-19b for HF patients versus controls and HFrEF versus HFpEF were obtained by ROC analysis and described by area under curve (AUC), sensitivity and specificity. Results: HFrEF patients (0.87, 95% CI 0.37-1.45) had significantly lower miR-19b level than HFpEF group (1.32, 95% CI 0.63-2.51) and the controls (1.82, 95% CI 0.37-1.45) (both P < .001). There was a remarkable negative correlation between miR-19b and NT-proBNP (P < .001). The additional use of miR-19b did not improve the accuracy of NT-proBNP alone in diagnosing HF patients from the controls (both AUC = 0.98, 95%CI 0.97-0.99). However, as for distinguishing the HFpEF from HFrEF, miR-19b and NT-proBNP yielded a significantly higher AUC than NT-proBNP alone (0.85, 95% CI 0.80-0.90 vs. 0.66, 95% CI 0.58-0.74) (P < .001), and the sensitivity for diagnosing HFrEF was raised from 58% to 77% and the specificity from 75% to 79%. Conclusions: On top of NT-proBNP, miR-19b added the value in diagnosing HFrEF. But in view of satisfactory accuracy of NT-proBNP in predicting HF from the healthy volunteers, miR-19b did not provide incremental value.

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Section: Quantitative Assessment Of Mirna-19bmentioning

confidence: 99%

Diagnostic value of circulating microRNA‐19b in heart failure

Zhang

Liu

et al. 2020

Eur J Clin Investigation

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“…In the present study we demonstrated that the human chronic indeterminate phase of Chagas disease results in increased levels of circulating miR-208a, an essential regulator of the genes involved in cardiac hypertrophy and fibrosis (Cunha-Neto et al, 2005 ; Wang et al, 2013 ; Doka et al, 2015 ; Shyu et al, 2015 ; Zhang et al, 2017 ). MiR-208a is encoded by an intronic region of the Myh6 gene that encodes α-myosin heavy chain, the prevalent heavy-chain contractile protein in the developed adult heart (Cunha-Neto et al, 2005 ).…”

Section: Discussionmentioning

confidence: 67%

“…MiR-208a is encoded by an intronic region of the Myh6 gene that encodes α-myosin heavy chain, the prevalent heavy-chain contractile protein in the developed adult heart (Cunha-Neto et al, 2005 ). Alterations in the miR-208a expression levels are frequently associated with pathological heart dysfunctions, such as hypertrophy, fibrosis, arrhythmias, contractile dysfunction and conduction abnormalities (Cunha-Neto et al, 2005 ; Wang et al, 2013 ; Doka et al, 2015 ; Shyu et al, 2015 ; Zhang et al, 2017 ). Indeed, overexpression of miR-208a is associated with coronary heart disease (Zhang et al, 2017 ), and its therapeutic inhibition leads to recovery of cardiac function during heart disease (Doka et al, 2015 ).…”

Section: Discussionmentioning

confidence: 99%

“…Alterations in the miR-208a expression levels are frequently associated with pathological heart dysfunctions, such as hypertrophy, fibrosis, arrhythmias, contractile dysfunction and conduction abnormalities (Cunha-Neto et al, 2005 ; Wang et al, 2013 ; Doka et al, 2015 ; Shyu et al, 2015 ; Zhang et al, 2017 ). Indeed, overexpression of miR-208a is associated with coronary heart disease (Zhang et al, 2017 ), and its therapeutic inhibition leads to recovery of cardiac function during heart disease (Doka et al, 2015 ). It has been shown that transforming growth factor-β (TGF-β) activates mirR-208a to regulate hypertrophic-related genes and the effect of neutralizating TGF-β antibody attenuates miR-208a induced-expression in cardiomyocyte hypertrophy (Wang et al, 2013 ).…”

Section: Discussionmentioning

confidence: 99%

“…MiR-208a is one of the most important heart-specific miRNA playing a critical role in the heart failure and has been considered as a potential biomarker of myocardial injuries (Cunha-Neto et al, 2005 ; Wang et al, 2013 ; Doka et al, 2015 ; Shyu et al, 2015 ; Zhang et al, 2017 ). The miR-208a is encoded by an intronic region of the alpha-cardiac muscle myosin heavy chain gene (Myh6) regulating the myosin heavy chain isoform switch (Cunha-Neto et al, 2005 ).…”

Section: Introductionmentioning

confidence: 99%

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Circulating Plasma MicroRNA-208a as Potential Biomarker of Chronic Indeterminate Phase of Chagas Disease

et al. 2018

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Chagas cardiomyopathy is the most severe clinical manifestation of chronic Chagas disease. The disease affects most of the Latin American countries, being considered one of the leading causes of morbidity and death in the continent. The pathogenesis of Chagas cardiomyopathy is very complex, with mechanisms involving parasite-dependent cytopathy, immune-mediated myocardial damage and neurogenic disturbances. These pathological changes eventually result in cardiac myocyte hypertrophy, arrhythmias, congestive heart failure and stroke during chronic infection phase. Herein, we show that miR-208a, a microRNA that is a key factor in promoting cardiovascular dysfunction during cardiac hypertrophy processes of heart failure, has its circulating levels increased during chronic indeterminate phase when compared to cardiac (CARD) clinical forms in patients with Chagas disease. In contrast, we have not found altered serum levels of miR-34a, a microRNA known to promote pro-apoptotic role in myocardial infarction during degenerative process of cardiac injuries thus indicating intrinsic differences in the nature of the mechanisms underlying the heart failure triggered by Trypanosoma cruzi infection. Our findings support that the chronic indeterminate phase is a progressive phase involved in the genesis of chagasic cardiopathy and point out the use of plasma levels of miR-208a as candidate biomarker in risk-prediction score for the clinical prognosis of Chagas disease.

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Elevated serum miR‐3129‐5p contributes to the progression of coronary heart disease via targeting mTOR

Wang

Zhao

Zhou

et al. 2020

The Kaohsiung J of Med Scie

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The current study aims to explore the miRNA changes that occur in the serum of patients with coronary heart disease (CHD) and healthy controls using a microarray technique, thereby exploring the potential biomarkers in the diagnosis of CHD and the underlying mechanism. Clinical data were reviewed, and venous blood samples were collected from 66 cases of CHD and 58 cases of healthy controls. MicroRNAwide expression profiling identified 16 miRNAs that were aberrantly decreased bỹ 2-fold in the serum of patients with CHD compared to that of healthy controls. RT-PCR analysis indicated that the expression of miR-3129-5p was increased the most in patients with CHD compared with that of controls. Moreover, serum miR-3129-5p was found to be highest in the severe stenosis group, followed by the moderate stenosis group and mild stenosis group. ROC analysis showed that serum miR-3129-5p could differentiate patients with CHD from controls. Further study showed that mTOR was a target gene of miR-3129-5p. Western blot assays demonstrated that miR-3129-5p significantly suppressed the phosphorylation of S6 but increased LC3II/LC3I and Beclin1 levels. Consistently, GFP-LC3 and TEM assays indicated that miR-3129 increased autophagy puncta in H9C2 cells. More importantly, silencing mTOR significantly decreased the expression of p-S6 but increased LC3II/LC3I and Beclin expression even in H9C2 cells transfected with miR-3129-5p inhibitor, indicating that miR-3129-5p-induced cell autophagy was mediated via mTOR in H9C2 cells. In summary, elevated serum miR-3129-5p contributes to CHD by targeting mTOR signaling and may be a therapeutic target in the treatment of CHD.

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Association between circulating microRNA-208a and severity of coronary heart disease

Cited by 37 publications

References 23 publications

Diagnostic value of circulating microRNA‐19b in heart failure

Diagnostic value of circulating microRNA‐19b in heart failure

Circulating Plasma MicroRNA-208a as Potential Biomarker of Chronic Indeterminate Phase of Chagas Disease

Elevated serum miR‐3129‐5p contributes to the progression of coronary heart disease via targeting mTOR

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