Association between density of tumor-infiltrating lymphocytes and prognoses of patients with gastric cancer

Abstract

Introduction:Tumor-infiltrating lymphocytes (TILs) have been shown to be of prognostic significance in patients with gastric cancer. This study aims to investigate the association between density of TILs and prognoses of patients with gastric cancer.Methods:The relative studies of tumor-infiltrating lymphocytes in tumor tissue from patients with gastric cancer were systematically searched from PubMed and Embase until October 31, 2017. The pooled hazard ratios (HRs) and their 95% confidence intervals (95%CI) for overall survival (OS) were estimated.Results:Twenty-nine studies involving 4,942 patients were included into analyses. Subset of TILs included CD8+, CD3+, CD4+, and FOXP3+ T cell density. Results from meta-analyses revealed that high density of intratumoral CD8+ T cells (HR = 0.77, 95% CI 0.63–0.95) and CD3+ (HR = 0.62, 95% CI 0.49–0.77) were associated with significantly higher OS than those with low density in patients with gastric cancer. Moreover, a larger number of general TILs density also suggested a favorable prognosis (HR 0.75, 95% CI 0.67–0.84). However, patients with high density of intratumoral FOXP3+ T or CD4+ T cells were not statistically associated with higher or lower OS than those with low density (HR 1.41, 95% CI 0.97–2.05; HR = 0.86, 95% CI 0.47–1.57). Sample size and follow-up period seemed to influence study outcomes.Conclusion:The present study revealed that high density of intratumoral CD8+ and CD3+ T cells were associated with better OS in patients with gastric cancer.

Exploring TCGA database for identification of potential prognostic genes in stomach adenocarcinoma

“…First, we obtained 245 up-regulated genes and 103 down-regulated genes by analysing the differences between high-and low-score group samples. Following functional enrichment analysis of the up-and down-regulated genes, it was found that many genes are associated with the TME, which is consistent with previous studies reporting the important roles of immune cells and stromal cells in the TME [38][39][40][41]. A total of 10 core genes were extracted from the PPI network, and a module analysis was performed.…”

P2X7R as an independent prognostic indicator in gastric cancer

“…In a meta-analysis, Yu et al stated that TILs are prognostic markers for OS in patients with GC. Moreover, they revealed that intratumoral CD8+ and CD3+ lymphocyte density were associated with good prognosis in GC, whereas CD4+ and FOXP3+ lymphocyte density had no significant effect on survival outcomes [34]. In the present study, both OS and DFS times were significantly higher in patients with high CD8+ TILs density than those with low TILs density.…”

Gastric cancer: genome damaged by bugs

“…The satisfied response might rely on that EBVaGC expresses high levels of PD-L1 [165,170] and exhibits more tumor infiltrating lymphocytes (TILs) [163,167,171,172]. The amount of TILs has been reported to be associated with improved overall survival in GC patients [173]. In a research of advanced GC patients treated with nivolumab, only 25% of patients (1/4) demonstrated good response, and this might be because not all EBVpositive tumors show high PD-L1 expression [174].…”