Association between ERCC1 and ERCC2 gene polymorphisms and susceptibility to pancreatic cancer

Abstract

ABSTRACT. We conducted a study to investigate the association between ERCC1 (rs3212986) and ERCC2 (rs13181) gene polymorphisms and the risk of pancreatic cancer in a Chinese population. A total of 217 pancreatic cancer patients and 244 control subjects were recruited from the Nuclear Industry 215 Hospital of Shaanxi Province between February 2013 and December 2014. Genomic DNA was extracted from peripheral blood samples using a TIANamp Blood DNA Kit (Tiangen, Beijing, China) according to the manufacturer's instructions. The ERCC1 rs3212986 and ERCC2 rs13181 polymorphisms were genotyped by polymerase chain reaction-restriction fragment length of polymorphism. Unconditional logistic regression analyses showed that subjects with the CC genotype of ERCC1 rs3212986 were susceptible to the development of pancreatic cancer when compared with subjects with the AA genotype (OR = 2.57, 95%CI = 1.34-5.02). The ERCC1 rs3212986 gene polymorphism was associated with increased risk of pancreatic cancer in the dominant (OR = 1.54, 95%CI = 1.05-2.28) and recessive (OR = 2.22, 95%CI = 1.20-4.19) models. However, no significant difference was found between the ERCC2 rs13181 polymorphism and the risk of pancreatic cancer in the codominant, dominant, and recessive models. We suggest that the ERCC1 rs3212986 polymorphism increases susceptibility to pancreatic cancer in the codominant, dominant, and recessive models, although further studies are needed to confirm our findings.

Short-term prognosis of childhood hepatoblastoma in relation to ERCC1 C118T single nucleotide polymorphism and VEGF expression

“…It is not difficult to infer that the children with CT + TT had a reduced ability to repair nucleotide excision; therefore, they had a lower ability to repair PD-induced damage, were more sensitive to PDs, and exhibited better chemotherapy results. The above inference is consistent with the reports of other studies both in and outside China [8][9][10][11][12][13].…”

“…Patients with the T allele may also be more sensitive to PDs [31,32]. Globally, many studies in non-small cell lung cancer [8], ovarian cancer [9], gastric cancer [10], esophageal cancer [11], pancreatic cancer [12], and colorectal cancer [13] have shown patients with the CT or TT genotypes in ERCC1 C118T mutation to be more sensitive to PDs when compared to patients with the CC genotype.…”

“…ERCC1 single nucleotide polymorphisms (SNPs), particularly the C-T mutation at the 118 th codon in exon 4, can affect gene expression level and change gene copy number, thus affecting the resistance to PDs [8]. Many studies have reported that the ERCC1 C118T mutation exhibits a predictive value for PD efficacy and disease prognosis in different types of cancers, such as non-small cell lung cancer [8], ovarian cancer [9], gastric cancer [10], esophageal cancer [11], pancreatic cancer [12], and colorectal cancer [13]. Therefore, screening ERCC1 mutation before chemotherapy may have important guiding significance and prognostic value for chemotherapy.…”

Association between ERCC2 Lys751Gln polymorphism and the risk of pancreatic cancer, especially among Asians: evidence from a meta-analysis

“…There are many studies which focus on the relationship between this SNP and pancreatic cancer [ 11 – 18 ]. Jiao et al (2007), Hocevar et al (2014), Ying et al (2015) and He et al (2016) did not observe any significant association between rs13181 and the risk of pancreatic cancer [ 11 – 14 ]. However, McWilliams et al (2008), Zhao et al (2015), Yan et al (2016) and Sileng et al (2016) suggested that this polymorphism was associated with an increased susceptibility to pancreatic cancer [ 15 – 18 ].…”

Effect of Interferon-γ Polymorphisms on Ankylosing Spondylitis: A Case-Control Study

“…Genomic DNA was extracted using the TIANamp genomic DNA Kit purchased from TIANGEN BIOTECH CO., LTD (Beijing) [ 21 ], according to the manufacturer’s instructions, and was stored at −20°C.…”