Association between glucosinolate concentration and injuries caused by cabbage stink bugs Eurydema spp. (Heteroptera: Pentatomidae) on different Brassicas

Abstract

In 2010, we were determining the contents of glucosinolates in different Brassicas in order to study their influence on feeding of cabbage stink bugs (Eurydema spp.) and the consequent extent of damage. We confirmed that glucosinolates content depends on plant species, plant organs and the time of sampling. In the samples aliphatic glucosinolates (glucoiberin, progoitrin, epiprogoitrin, epiprogoitrin, sinigrin, gluconapin, glucoraphenin, sinalbin) prevailed. Glucobrassicin, an important indolic glucosinolate compound, was detected in all tested Brassicas. Its concentration in the oil radish samples was highest during the first assessment (30 DAS), 8.84 ± 0.65 μmol g -1 ds, while the oilseed rape samples displayed lowest concentration during the last assessment (134 DAS), 4.30 ± 0.80 μmol g -1 ds. The stimulative activity of individual glucosinolates or their negative influence on feeding of cabbage stink bugs in the Brassicas used in our experiment was not uniformly manifested. Based on a two-year field experiment we concluded that oil rape was the most adequate trap crop used to allure cabbage stink bugs. In future, glucosinolates should be employed to a greater extent in environmentally acceptable ways of food production, one of which is also the use of trap crops in order to reduce harmful effects of cabbage stink bugs.Associação entre a concentração glucosinolada e danos causados pelo percevejo Eurydema spp. (Heteroptera: Pentatomidae) em diferentes gramíneas RESUMO. Em 2010 o conteúdo de glucosinolatos em diferentes plantas brássicas foi determinado para estudar a influência sobre a alimentação de percevejos de repolho (Eurydema spp.) e a extensão do dano causado. Através do método do cultivo armadilha, durante o período de crescimento foram amostradas as partes aéreas de óleo de colza, mostarda branca, nabo forrageiro e dois híbridos de repolho branco. O conteúdo de glucosinolatos depende das espécies de plantas, órgãos de plantas e período da amostragem. Predominaram nas amostras os glucosinolatos alifáticos (glucoiberina, progoitrina, epiprogoitrina, sinigrina, gluconapina, glucoraphenina, sinalbina). A glucobrassicina foi detectada em todas as brássicas testadas. Sua concentração no nabo forrageiro foi mais alta na primeira avaliação (30 DAS), 8.84 ± 0.65 μmol g -1 ds, enquanto houve a menor concentração no óleo de colza durante a última avaliação (134 DAS), 4.30 ± 0.80 μmol g -1 ds. Considerando o experimento de dois anos, conclui-se que o óleo de colza foi o mais adequado como cultivo armadilha para atrair o percevejo do repolho. Glucosinolatos podem ser usados no futuro mais extensivamente em várias modalidades de produção de alimentos, entre as quais o emprego de cultivo armadilha para diminuir os danos do percevejo do repolho.Palavras-chave: fitoquímicos, percevejo do repolho, repolho, nabo forrageiro, mostarda branca.

Glucosinolate Profiles in Cabbage Genotypes Influence the Preferential Feeding of Diamondback Moth (Plutella xylostella)

“…Additionally, despite a few previous reports of contrasting roles of two classes of glucosinolates namely, aliphatics and indolics in conferring resistance or susceptibility against insect herbivory ( De Vos et al, 2008 ; Gols et al, 2008 ; Newton et al, 2009 ; Kos et al, 2011 ; Bohinc et al, 2013 ), our PCA-based approach didn’t recognize any definitive patterns in the contents of either of these two classes of glucosinolates and feeding preference of DBM larvae. This observation suggested that DBM’s feeding preference is not restricted to any particular classes of glucosinolates in our study.…”

“…This observation suggested that DBM’s feeding preference is not restricted to any particular classes of glucosinolates in our study. Together, all these results of laboratory and field experiments indicated that the insect–herbivore interaction is dynamically dependent on the genotype specific glucosinolate profiles as evident by its differential influence on the feeding behavior and damage levels of various generalist and specialist insects ( Mauricio, 1998 ; Li et al, 2000 ; Barth and Jander, 2006 ; Bohinc et al, 2013 ).…”

Bottom-up and top-down herbivore regulation mediated by glucosinolates in Brassica oleracea var. acephala

“…The role of sinigrin as an oviposition stimulant for the E. ornatum female has never been reported in the past. A previous study on the responses of this hemipteran to the glucosinolate pattern of different Brassica crops did, however, highlight the importance of having a high content of aliphatic compounds, such as progoitrin, epiprogoitrin, gluconapin and glucoraphanin, and of the indole glucobrassicin in order to reduce the extent of damage, although the effects also depended on plant age (Bohinc et al 2013).…”

About Previous Investigations Regarding the Role of Glucosinolates in Controlling Brassica Insect Pests in Slovenia

“…As our research shows, the method is more efficient in systems of production where mid-late genotypes of cabbage are grown. As we found out, due to the spectrum of different factors (among others also glucosinolate content [45,46]), susceptibility of plants to damage by harmful organisms during the growth period varies. By using mixed crops as a means to reduce damage on the main crops, the said method could be used in different regions of Slovenia and thus override the influence of environmental factors on trap crops as well as the bionomy of the said harmful species itself [47].…”