Association betweenATP2B1andCACNB2polymorphisms and high blood pressure in a population of Lithuanian children and adolescents: a cross-sectional study

Abstract

ObjectivesRecently, genome-wide associated studies have identified several genetic loci that are associated with elevated blood pressure and could play a critical role in intracellular calcium homeostasis. The aim of this study was to assess the associations of ATP2B1 rs2681472 and CACNB2 rs12258967 gene polymorphisms with high blood pressure (HBP) among Lithuanian children and adolescents aged 12–15 years.Study design and participantsThis was a cross-sectional study of a randomly selected sample of 646 12–15-year-old adolescents who participated in the survey ‘The Prevalence and Risk Factors of HBP in 12–15 Year-Old Lithuanian Children and Adolescents (from November 2010 to April 2012)’. Anthropometric parameters and BP were measured. The participants with HBP were screened on two separate occasions. Subjects were genotyped ATP2B1 rs2681472 and CACNB2 rs12258967 gene polymorphisms using real-time PCR method.ResultsThe prevalence of HBP was 36.7%, significantly higher for boys than for girls. In the multivariate analysis, after adjustment for body mass index and waist circumference, boys with CACNB2 CG genotype, CACNB2 GG genotype and CACNB2 CG +GG genotype had higher odds of having HBP in codominant (adjusted OR (aOR)=1.92; 95% CI 1.16 to 3.18, p=0.011; and aOR=2.64; 95% CI 1.19 to 5.90, p=0.018) and in dominant (aOR=2.05; 95% CI 1.27 to 3.30, p=0.003) inheritance models. Girls carrying CACNB2 CG genotype and CACNB2 CG +GG genotype had increased odds of HBP in codominant (aOR=1.82; 95% CI 1.02 to 3.24, p=0.044) and in dominant (aOR=1.89; 95% CI 1.09 to 3.28, p=0.023) inheritance models. Furthermore, significant associations were found in additive models separately for boys (aOR=1.72; 95% CI 1.20 to 2.46, p=0.003) and girls (aOR=1.52; 95% CI 1.05 to 2.20, p=0.027). No significant association was found between ATP2B1 gene polymorphism and the odds of HBP.ConclusionsOur results indicate that CACNB2 gene polymorphism was significantly associated with higher odds of HBP in Lithuanian adolescents aged 12–15 years.

Association between gene polymorphisms of voltage-dependent Ca2+ channels and hypertension in the Dai people of China: a case-control study

“…Although the association analyses in the Chinese Han population [14,15] and other countries [16,17] suggested that the CACNA1A (rs8182538), CACNA1C (rs758116) and the CACNB2 (rs4373814, rs11014166, rs12258967) polymorphisms were associated with blood pressure and hypertension (no reports about CACNA1S polymorphisms), these SNPs were not consistent with our pre-experimental results (200 cases vs 200 controls, unpublished results). Therefore, based on the haplotype analysis results of the Beijing Han (Chinese Han in Beijing (CHB), HapMap) [18], we chose another 17 SNPs in the genes (CACNA1A, CACNA1C, CACNA1S, CACNB2) that encode the proteins of VDCCs to perform genotyping in the Dai population.…”

“…Association analyses in China [14], Lithuanian [17] and Europe [16] have reported the association between CACNB2 polymorphisms (rs4373814 in Chinese Han, rs12258967 in Lithuanian and rs18748804 in Europe) and hypertension. In our study, we found three other positive SNPs (rs17539088, G minor , OR Dominant = 0.732 CI95% Dominant = 0.554-0.966, OR Additive = 0.769 CI95% Additive = 0.616-0.960; rs16917217, G minor , OR Dominant = 0.737 CI95% Dominant = 0.558-0.973, OR Additive = 0.776 CI95% Additive = 0.622-0.969; and rs61839222, A minor , OR Dominant = 0.737 CI95% Dominant = 0.558-0.973, OR Additive = 0.776 CI95% Additive = 0.622-0.969), which suggest a decreased risk, in CACNB2 after logistic regression analyses.…”

Association between gene polymorphisms of voltage-dependent Ca2+ channels and hypertension in the Dai people of China: a case-control study

“…Although the association analyses in the Chinese Han population [14,15] and other countries [16,17] suggested that the CACNA1A (rs8182538), CACNA1C (rs758116) and the CACNB2 (rs4373814,…”

Association between gene polymorphisms of voltage-dependent Ca2+ channels and hypertension in the Dai people of China: a case-control study

“…Although the association analyses in the Chinese Han population [14,15] and other countries [16,17] suggested that the CACNA1A (rs8182538), CACNA1C (rs758116) and the CACNB2 (rs4373814, The sequencing images were transformed into sequenced reads through base recognition analysis, and final identification of SNPs was conducted by Samtools 0.1.19 [20].…”

“…Kudryavtseva O et al [26]. investigated the effect of VDCCs on VSMC differentiation by injecting alpha Association analyses in China [14],Lithuanian [17]and Europe [16]have reported the association between CACNB2 polymorphisms (rs4373814 in Chinese Han, rs12258967 in Lithuanian and rs18748804 in Europe) and hypertension. In our study, we found three other positive SNPs (rs17539088, rs16917217 and rs61839222) in CACNB2 in a Dai population after logistic regression analyses.…”