Association between<i>MMP3</i>and<i>TIMP3</i>polymorphisms and risk of osteoarthritis

Tong, Zheming; Liu, Yang; Chen, Bin; Yan, Liang; Hao, Dingjun

doi:10.18632/oncotarget.18745

Cited by 26 publications

(28 citation statements)

References 37 publications

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“…In bone, TIMP-3 plays the role as a local cytokine, regulating bone metabolism through suppressing osteoblast differentiation and inducing osteoblast apoptosis [29,30]. TIMP3 was reported to be associated with RA and arthritis based on a search of the U. S. National Library of Medicine database (MED-LINE) (http://www.ncbi.nlm.nih.gov/IEB/Research/Ace mbly/av.cgi) [31]. A case-control study in a Chinese Han population linked TIMP3 polymorphism with severe knee OA [32].…”

Section: Discussionmentioning

confidence: 99%

Identification of differentially expressed miRNAs and mRNAs in synovial of osteoarthritis via RNA-sequencing

et al. 2020

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Background: Osteoarthritis (OA) is the most common form of arthritis and a leading cause of disability. This study attempted to investigate the key mRNAs and miRNAs related to OA. Patients and methods: From April 17th, 2018 to May 17th, 2018, five patients with OA and three normal controls were enrolled in this present study. To identify the differentially expressed mRNAs (DEmRNAs) and miRNAs (DEmiRNAs) between patients with OA and normal controls, RNA-sequencing was performed. Then, DEmiRNAtarget DEmRNAs analysis and functional annotation of DEmiRNA-target DEmRNAs were performed. To validate the RNA-sequencing results, quantitative real time-PCR (RT-PCR) and western blot analysis were performed as well. Results: A total of 1068 DEmRNAs, 21 DEmiRNAs and 395 DEmiRNA-DEmRNA pairs were identified in synovial tissues of patients with OA. The functional annotation of DEmiRNA-target DEmRNAs revealed that Pathways in cancer and PI3K-Akt signaling pathway were significantly enriched Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways. QRT-PCR and western blot results revealed that except for TLR7, the expression level of the others was consistent with the RNA-sequencing results, generally. Conclusion: The findings of this present study may provide new clues for the roles of DEmRNAs and DEmiRNAs in the pathogenesis of OA.

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Section: Discussionmentioning

confidence: 99%

Identification of differentially expressed miRNAs and mRNAs in synovial of osteoarthritis via RNA-sequencing

et al. 2020

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“…Col2a1 expression serves an important role in maintaining the normal function of chondrocytes, the downregulation of which indicates the degeneration of articular cartilage ( 21 – 23 ). MMP-3 is an important chondroitin-degrading enzyme whose upregulation promotes the degeneration of articular cartilage ( 24 ). In the present study, compared with normal control group, the expression of Col2a1 in OA chondrocytes was significantly reduced at the miRNA and protein levels, which is consistent with the results of previous studies ( 25 – 27 ).…”

Section: Discussionmentioning

confidence: 99%

Abnormal expression of miR‑4784 in chondrocytes of osteoarthritis and associations with chondrocyte hyperplasia

Liu

Yanhui

et al. 2018

Exp Ther Med

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The aim of the present study was to assess the expression of microRNA (miRNA)-4784 in the chondrocytes of early osteoarthritis (OA) and to determine the effect of double-stranded (ds)-miRNA-4784 transfection on chondrocyte function. Following the construction of an OA rabbit model, normal chondrocytes (normal control group), OA chondrocytes obtained 4 weeks after modeling (OA at week 4 group) and 8 weeks after modeling (OA at week 8 group) were used. The relative expression of miRNA-4784 in each group was detected using reverse transcription-quantitative polymerase chain reaction (RT-qPCR). Western blotting was performed to measure the expression of type II collagen (Col2a1) and matrix metalloproteinase (MMP)-3 in each group with or without ds-miRNA-4784 transfection. The results revealed that the levels of miR-4784 in groups OA at week 4 and 8 were significantly lower than that of normal control group (P<0.05). It was also demonstrated that Col2a1 mRNA expression levels in groups OA at week 4 and 8 were 49 and 38% of that in the normal control group, respectively. Furthermore, MMP-3 mRNA expression levels increased by 3.12- and 3.95-fold in groups OA at week 4 and 8, respectively, compared with those in the normal control group (P<0.01). Following transfection with ds-miRNA-4784, Col2a1 mRNA expression levels increased by 63 and 126% compared with the levels prior to treatment in groups OA at week 4 and 8, respectively (P<0.01). The expression levels of MMP-3 mRNA in groups OA at week 4 and 8 decreased following transfection compared with the levels prior to treatment. Col2a1 and MMP-3 protein expression exhibited similar patterns to the mRNA expression. In summary, the results of the present study suggest that miRNA-4784 expression is significantly reduced in early stage OA chondrocytes. Transfection with ds-miRNA-4784 promotes the expression of Col2a1 and inhibits the MMP-3 expression in chondrocytes.

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“…Matrix metalloproteinase-1 (MMP-1)is produced by synovial cells, chondrocytes, and osteoblasts, which can degrade extracellular matrix collagen and mediate cartilage destruction [7][8]. The expression of MMPs has been shown to be affected by single nucleotide polymorphisms (SNPs) occurring in MMP gene promoters [9].These promoter polymorphisms have allele-speci c effects on the regulation of MMP gene transcription and are associated with the development and/or progression of some common diseases [3][4][5]. Matrix metalloproteinases secreted from chondrocytes are the main enzymes responsible for pathological cartilage destruction [6].Expression of MMP-1 is low in normal cells that leads to healthy cartilage remodeling.…”

Section: Introductionmentioning

confidence: 99%

Association Between The Polymorphism of MMP-1 Gene with Knee Osteoarthritis Risk: A Meta-Analysis

Lu¹,

Sun²,

Wang³

et al. 2020

Preprint

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Background: The existing studies on the association between polymorphisms of Matrix metalloproteinase-1 (MMP-1) (-1607 1G/2G) (rs1799750) polymorphism and the risk of knee osteoarthritis (OA, a complex multifactorial disease and a major degenerative form of arthritis) in different populations have yielded conflicting findings. Method: Literature search was performed in PubMed, Cochrane Library, Web of science, Google Scholar (From January 1990 to June 2019), and assessing this association by calculating odds ratios with 95% confidence intervals. Subgroup analyses stratified by ethnicity were also conducted. Statistical heterogeneity was quantitatively evaluated by X2 test with the significance set P<0.10 or I2>50%.Result: Five case-control based studies (924 cases and 928 controls) were included. The results suggested that the MMP-1 (-1607 1G/2G) (rs1799750) gene polymorphisms were not associated with knee OA risk in all genetic models (Allele model OR =1.22, 95% CI: 0.72-1.76, p=0.615, Recessive model OR = 1.12, 95% CI: 0.70-2.15, p=0.486, and Dominant model OR = 1.14, 95%CI: 0.64-2.04, p=0.659, Figure 3-5)Conclusions: There is no association between the polymorphism of MMP-1 (-1607 1G/2G) (rs1799750) polymorphism with the risk of knee osteoarthritis, a large number of studies may be necessary to verify this association in different populations and environmental factors.

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Association betweenMMP3andTIMP3polymorphisms and risk of osteoarthritis

Cited by 26 publications

References 37 publications

Identification of differentially expressed miRNAs and mRNAs in synovial of osteoarthritis via RNA-sequencing

Identification of differentially expressed miRNAs and mRNAs in synovial of osteoarthritis via RNA-sequencing

Abnormal expression of miR‑4784 in chondrocytes of osteoarthritis and associations with chondrocyte hyperplasia

Association Between The Polymorphism of MMP-1 Gene with Knee Osteoarthritis Risk: A Meta-Analysis

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