Association betweenvitamin D receptor genepolymorphisms and chronic periodontitis among Libyans

Abstract

BackgroundChronic periodontitis (CP) is a common oral disease characterized by inflammation in the supporting tissue of the teeth ‘the periodontium’, periodontal attachment loss, and alveolar bone loss. The disease has a microbial etiology; however, recent findings suggest that the genetic factors, such as vitamin D receptor (VDR) gene polymorphisms, have also been included.AimInvestigation of the relationship between VDR gene polymorphisms and CP among Libyans.Materials and methodsIn this study, we examined 196 unrelated Libyans between the ages of 25 and 65 years, including 99 patients and 97 controls. An oral examination based on Ramfjord Index was performed at different dental clinics in Tripoli and information were collected using a self-reported questionnaire. DNA was extracted from buccal swabs; the VDR ApaI, BsmI, and FokI polymorphisms were genotyped using polymerase chain reaction and were sequenced using Sanger Method.ResultsA significant difference in the newly detected ApaI SNP C/T rs#731236 was found (p=0.022), whereas no significant differences were found in ApaI SNP G/T rs#7975232, BsmI SNP A/G rs#1544410, and FokI SNP A/G rs#2228570 between patients and controls (p=0.939, 0.466, 0.239), respectively.ConclusionVDR ApaI SNP C/T rs#731236 may be related to the risk of CP in the Libyan population.

Association of vitamin D receptor gene polymorphism (rs10735810) and chronic periodontitis

“…El Jilani et al reported that there was no significant association between CP and FokI A/G SNP rs2228570 in a Libyan population which was in accordance with our present study. A study performed by Arroyave et al in a Colombian population showed that although the rs2228570 ( FokI ) polymorphism remains a potentially functional variant, there was no association either between the different genotype/allele frequencies or haplotype and CP; it does not appear to be in linkage disequilibrium with the BsmI , ApaI or TaqI polymorphic sites, which was in accordance with our study.…”

Influence of rs2228570 on Transcriptional Activation by the Vitamin D Receptor in Human Gingival Fibroblasts and Periodontal Ligament Cells

“…Similarly, a Chinese study of 51 patients with generalized AgP and 53 healthy controls concluded that the FF‐VDR (OR = 2.90) and the F allele (OR = 2.02) might increase susceptibility of AgP 20 . In another study, 99 patients with CP and 97 healthy controls in Libya were investigated, and no association between rs2228570 and CP was detected 21 . Recently, FF and Ff genotypes were associated with increased susceptibility to severe CP in 1,460 Thai patients 22 .…”

“…To date, only a few studies 18–22 have focused on the relationship between rs2228570 and periodontitis. In 2003, 74 patients with chronic periodontitis (CP) and 94 healthy controls were studied, but no association between rs2228570 and CP was observed 18 .…”

Preliminary investigation on the molecular mechanisms underlying the correlation between VDR‐FokI genotype and periodontitis

“…12 In a Libyan study of 99 CP patients and 97 controls, the lack of association between rs2228570 and CP was reported again. 13 On the contrary, in a Thai study with a large sample size of 1460 participants, both FF and Ff genotypes were reported to be more susceptible to severe CP. 14 Recently, it was revealed by a Romanian study including 53 CP patients and 47 controls that both FF and Ff genotypes were more likely to develop CP than ff genotype, 15 which is in line with the results of the Thai study.…”

“…Similarly, in a Chinese study of 107 severe CP patients and 121 controls, no association between rs2228570 and severe CP was reported . In a Libyan study of 99 CP patients and 97 controls, the lack of association between rs2228570 and CP was reported again . On the contrary, in a Thai study with a large sample size of 1460 participants, both FF and Ff genotypes were reported to be more susceptible to severe CP .…”