Association between IL-1β polymorphisms and gastritis risk

Sun, Xuemei; Cai, Hua; Li, Zhouru; Li, Shanshan; Yin, Wenjiang; Dong, Guokai; Kuai, Jinxia; He, Yuqing; Jia, Jing

doi:10.1097/md.0000000000006001

Cited by 15 publications

(16 citation statements)

References 36 publications

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“…Thus, IL-1 may be a crucial regulator of cartilage loss in arthritis. IL-1A (-889) and IL-1B (-511) variants locate in the promoter region of the genes, and they have been proved to affect the expression level of these genes [22,23].…”

Section: Discussionmentioning

confidence: 99%

Interleukin-1 gene variants and the risk of non-syndromic microtia

Bekerecioğlu¹,

Büyükgüral²,

Pehlıvan³

2018

Pau Med J

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Microtia is a congenital anomaly, manifested by a small and disfigured auricle. Interleukin (IL) 1 is an important mediator of inflammation and cartilage destruction, This study is aimed at investigating association of IL-1A (-889) and IL-1B (-511) variants in a Turkish patient population with microtia. Meterials and Methods: Nineteen patients diagnosed with microtia and 40 healthy controls were enrolled to the study. The IL-1A (-889) and IL-1B (-511) variants were evaluated using the polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method. For statistical analysis, SPSS version 22.0 was used. Results: The genotype distribution of the IL-1A (-889) variant was statistically different between the cases and the control group. IL-1A-889 CC genotype was lower in microtia cases while TT genotype was more prevalent in microtia cases, respectively (p=0.008, p=0.008). High difference was also observed when the patient group and the control group were compared according to IL-1 (-889) CT+TT (p=0.003). IL-1A (-889) C allele was lower in microtia patients and T allele was higher in patients (p=0.005). The allele frequency and genotype distribution of IL-1B (-511) CT variant did not show any statistically difference between patients and controls (p>0.05). Conclusion: To our knowledge, for the first time in the literature we have demonstrated a significant association of the IL-1A (-889) functional variant with microtia in a Turkish cohort.

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Section: Discussionmentioning

confidence: 99%

Interleukin-1 gene variants and the risk of non-syndromic microtia

Bekerecioğlu¹,

Büyükgüral²,

Pehlıvan³

2018

Pau Med J

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“…13 Candida glabrata Fructose bisphosphate aldolase protein sequences (361aa proteins) were retrieved from NCBI (https://www.ncbi.nlm.nih.gov/protein) Database on 21 January 2019 [17] .…”

Section: Retrieving Of Fructose Bisphosphate Aldolase Protein Sequencesmentioning

confidence: 99%

“…Also, its genome contains more tandem repeats of genes than the other Nakaseomyces [13] C. glabrata covers 67 genes encoding putative adhesin which is cell wall proteins, including the Epa family with 17 members [11,14] . Most importantly examples are Epithelial Adhesin 1 (Epa1p) [15] and Fructose bisphosphate aldolase protein (Fba1) which play an essential role in the pathogenicity of Candida species mainly in the adhesion of the pathogen to the host [16,17] .…”

Section: Introductionmentioning

confidence: 99%

Epitope-Based Peptide Vaccine Design Against Fructose Bisphosphate Aldolase of Candida Glabrata: An Immunomics Approach

Elhasan

Hassan

Elhassan

et al. 2020

Preprint

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AbstractBackgroundCandida glabrata is a human opportunistic pathogen that can cause life-threatening systemic infections. Although, there are multiple effective vaccines against fungal infections, and some of these vaccines were engaged in different stages of clinical trials, none of them yet approved by (FDA).AimTo predict the most conserved and immunogenic B- and T-cell epitopes from the Fructose Bisphosphate aldolase (Fba1) protein of C. glabrata.Materials and Methods13 C. glabrata Fructose bisphosphate aldolase protein sequences (361amino acid) were retrieved from NCBI and several in silico tools presented in the IEDB server for predicting peptides were used and homology modeling and molecular docking were performed.ResultThe promising B-cell Epitopes were AYFKPH, VDKESLYTK, and HVDKESLYTK. While, promising peptides which have the high affinity to MHC I binding were: AVHEALAPI, KYFKRMAAM, QTSNGGAAY, RMAAMNQWL and YFKEHGEPL. Two peptides (LFSSHMLDL and YIRSIAPAY) were noted to have the highest affinity to MHC class II that interact with 9 MHC class II alleles. The molecular Docking revealed the epitopes QTSNGGAAY and LFSSHMLDL have the high binding energy to MHC moleculesConclusionThe epitope-based vaccines predicted by using immunoinformatics tools have remarkable advantages over the conventional vaccines that they are more specific, less time consuming, safe, less allergic and more antigenic. Further in vivo and in vitro experiments are needed to prove the effectiveness of the best candidates epitopes (QTSNGGAAY and LFSSHMLDL). To the best of our knowledge, this is the first study that has predicted B- and T-cells epitopes from Fba1 protein by using in silico tools in order to design an effective epitope-based vaccine against C. galabrata.

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“…Because of the ability of IL-1β to inhibit gastric acid secretion it may have a profound effect on the natural history of H. pylori infection by allowing expansion of H. pylori colonization from the gastric antrum to the corpus. (15,(18)(19)(20) On a molar basis, IL-1β a 100-fold more potent inhibitor than PPIs, and 6000-fold more potent than H2 receptor antagonists. (21) IL-1β is expressed at high levels in myeloid cell lineages in response to tissue injury and microbial invasion.…”

Section: Introductionmentioning

confidence: 99%

“…Many studies have been published analyzing the contribution of IL1B promoter polymorphisms to H. pylori susceptibility with con icting results explained, in part, by ethnic differences. (19,36,37). In the present study, genomic DNA Sanger sequencing was applied to detect SNPs in the region [-687_+297] of IL1B in H. pylori-infected patients; and bioinformatics analyses were used to study whether these mutations would alter transcription factor binding sites.…”

Section: Introductionmentioning

confidence: 99%

First Insights Into the Molecular Basis Association Between Promoter Polymorphisms of the Il1b Gene and Helicobacter Pylori Infection in the Sudanese Population: Computational Approach

Idris

Ataelmanan

et al. 2020

Preprint

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Background:Helicobacter pylori (H. pylori) infects nearly half of the world’s population with a variation in incidence among different geographic regions. Genetic variants in the promoter regions of IL1B gene can affect cytokine expression and creates a condition of hypoacidity which favors the survival and colonization of H. pylori.Aim: To characterize the polymorphic sites in the 5’- region [-687_+297] of IL1B in H. pylori infection using in silico tools.Methods: Genomic DNA was extracted from 121 gastric biopsies and genotyping of IL1B-31 polymorphism was performed using PCR-CTPP to investigate its association with the susceptibility to H. pylori infection in the Sudanese population. In addition, Sanger sequencing was applied to detect SNPs in the 5’-region [-687_+297] of IL1B in 14 H. pylori-infected patients; and bioinformatics analyses were used to predict whether these mutations would alter TFBSs or CEs in this region. Also, comparative analysis was conducted for this region in 11 species using ECR browser and Mulan search engine.Results: A total of five nucleotide variations were detected in the 5’-regulatory region which led to the addition or alteration of the TFBSs and CEs. Genotyping of IL1B-31 C>T revealed a significant association between -31T and susceptibility to H. pylori infection in the studied population (P=0.0280). Comparative analysis showed conservation rates of IL1B upstream [−368_+10] region above 70% in chimpanzee, rhesus monkey, a domesticated dog, cow and rat.Conclusion: In H. pylori-infected patients, three detected SNPs (-338, -155 and -31) located in the IL1B promoter were predicted to alter TFBSs and CE, thereby might affecting gene expression. These in silico predictions provide insight for further experimental in vitro and in vivo studies of the regulation of IL1B expression and its relationship to H. pylori infection. However, recognition of regulatory motifs by computer algorithms is fundamental for understanding gene expression patterns.

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Association between IL-1β polymorphisms and gastritis risk

Abstract: Supplemental Digital Content is available in the text

Cited by 15 publications

References 36 publications

Interleukin-1 gene variants and the risk of non-syndromic microtia

Interleukin-1 gene variants and the risk of non-syndromic microtia

Epitope-Based Peptide Vaccine Design Against Fructose Bisphosphate Aldolase of Candida Glabrata: An Immunomics Approach

First Insights Into the Molecular Basis Association Between Promoter Polymorphisms of the Il1b Gene and Helicobacter Pylori Infection in the Sudanese Population: Computational Approach

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