Association between manganese superoxide dismutase (MnSOD) gene polymorphism and elderly obesity

Abstract

Evidence suggests an association between obesity and oxidative stress caused by superoxide production. Since the dismutation of superoxide is catalyzed by superoxide dismutase enzymes, we tested the association between obesity and Ala16Val manganese-dependent superoxide dismutase gene (MnSOD) polymorphism. We analyzed 815 free-living community subjects (> or =60 years old) grouped into subjects who were either obese (BMI > or = 30 kg/m(2)) or non-obese (BMI < 25 kg/m(2)). Additionally, we investigated the possible interaction between the Ala16Val MnSOD gene polymorphism and obesity in the modulation of biochemical and nutritional variables. We found a positive association between MnSOD polymorphism and obesity, since higher VV frequency (28.2%) was observed in the obese group (P = 0.002, odds ratio 1.949, 95% CI: 1.223-3.008). This result was independent of sex, age, diabetes, dyslipidemia, hypertension, and metabolic syndrome. A possible biological explanation of the association described here could be a chronic state of superoxide enzyme imbalance present in VV carriers, which could affect differential metabolic pathways contributing to the obese state.

In vitro effects of Ala16Val manganese superoxide dismutase gene polymorphism on human white blood cells exposed to methylmercury

“…A subgroup of the initial study group was asked to donate blood again for in vitro tests because a previous study performed by Montano et al (2009), has shown an association between the Ala16Val polymorphism, dietary behavior, and health conditions. The 8 mL blood samples were collected via venipuncture using tubes with heparin, which were centrifuged within 1 h of collection for 15 min at 3000 rpm.…”

“…Interestingly, in some cases, the disease risk is associated with the A allele or AA genotype -for example, in prostate cancer (Taufer et al, 2005), breast cancer (Bica et al, 2009), and other cancers. Disease risk is linked to the V allele or VV genotype in diabetes microvascular complications (Tian et al, 2011), obesity (Montano et al, 2009), hypercholesterolemia (Duarte et al, 2010), and metastatic potential of breast cancer (Bica et al, 2010).…”

Impact of obesity and Ala16Val MnSOD polymorphism interaction on lipid, inflammatory and oxidative blood biomarkers

“…The Ala16Val MnSOD gene polymorphism was determined as described in Montano et al [14]. Briefly, genomic DNA was isolated from peripheral blood leukocytes using a DNA Mini Kit Purification (Mo Bio).…”

“…Previously, Montano et al [14] described positive association between VV genotype and obesity and other studies also described association with hypercholes-terolemia [15], diabetes or diabetic cardiovascular diseases [16,17] diabetic nephropathy [18] and diabetic retinopathy [19], elevate plasmatic oxided-LDL levels mainly in diabetic patients [20], elevate also lipid peroxidation, protein carbonylation [10,21] and proinflammatory-cytokines (IL-1β, IL-6, TNFα and Igɣ) [22].…”

Apolipoprotein E polymorphism in elderly Japanese-Brazilian immigrants does not explain the reduced cardiovascular risk factor incidence

“…The elderly native group (GP group) was selected from the Southern Brazilian Aging Research Program, which investigates genetic and environmental interactions in aging and related non-transmissible diseases. Previous studies have been published and described in detail about this research project (da Cruz et al, 2003;Prado-Lima et al, 2006;Montano et al, 2009;Duarte et al, 2010). Exclusion criteria were: <60 years old and previous chronic diseases that could influence the analysis performed, such as cancer, auto-immune diseases, etc.…”