Association between microRNAs 10b/21/34a and acute toxicity in glioblastoma patients treated with radiotherapy and temozolomide

Abstract

A personalized approach to chemoradiation is important in reducing its potential side effects and identifying a group of patients prone to toxicity. MicroRNAs have been shown to have a predictive potential for radiotoxicity. The goal of the study was to test if levels of miRNA in peripheral blood mononuclear cells of glioblastoma patients are associated with toxicity and to identify the peak time point for toxicity. MicroRNA-10b/21/34a levels were measured in 43 patients with and without toxicity, at baseline, at the 15th, and at the 30th fraction by Real-Time quantitative Polymerase Chain Reaction. MicroRNA-10b/21 levels increased with toxicity grade (p = 0.014; p = 0.013); miR-21/34a levels were significantly different between patients with and without toxicity at the 15th fraction (p = 0.030; p = 0.045), while miR-34a levels significantly changed during treatment (p < 0.001). All three miRNAs showed a significantly high positive correlation with one another. MiR-34a might be considered as a predictive factor for toxicity due to its changes during treatment, and differences between the groups with and without toxicity; miR-10b might be used to predict toxicity; miR-10b/21 might be used for predicting the grade of toxicity in GB patients.

MicroRNAs in high-grade gliomas: What is their role?

“…We measured and observed the expression levels of microRNA-10b/21/34a before radiotherapy and TMZ, at the 15th fraction of the concurrent treatment, and at the last fraction of radiation plus TMZ. Our results revealed higher expression levels of microRNA-10b/21 in patients with higher grades of toxicity (26). The level of expression of miR-34 significantly changed during concurrent treatment, as well (26).…”

“…Our study published in 2022, included 43 patients with GB treated with radiotherapy and TMZ (26). Twentytwo of those patients exhibited toxicity and twenty-one patient did not show any toxicity (26).…”

“…Our study published in 2022, included 43 patients with GB treated with radiotherapy and TMZ (26). Twentytwo of those patients exhibited toxicity and twenty-one patient did not show any toxicity (26). We measured and observed the expression levels of microRNA-10b/21/34a before radiotherapy and TMZ, at the 15th fraction of the concurrent treatment, and at the last fraction of radiation plus TMZ.…”

“…Stepanović A. et al MicroRNAs in high-grade gliomas: What is their role? MedPodml 2023, 74(2): [24][25][26][27][28][29] Visokogradusni gliomi su maligni tumori centralnog nervnog sistema sa lošim ukupnim preživljavanjem. Glioblastom ima najagresivnije biološko ponašanje.…”

Current aspects of radiobiology in modern radiotherapy - our clinical experience

“…Many studies of gene expression profiles in peripheral blood lymphocytes of RT-treated patients have indicated an association between certain differentially expressed genes and acute and late radiotoxicity [7]. Therefore, determining the expression profile of a large number of genes by the method of miRNA expression analysis or sequencing of new generation RNA is one of the most important research approaches in modern radiobiology, which allows the discovery of new potential biomarkers of RT effects [7,8,9]. Methodological approaches used in cellular radiosensitivity testing include assays to determine cell proliferation and survival, cytogenetic assays, methods to detect DNA damage, and apoptotic assays.…”