Association between  Mycoplasma genitalium  and acute endometritis

Cohen, Craig R.; Manhart, Lisa E.; Bukusi, Elizabeth A.; Astete, Sabina G.; Brunham, Robert C.; Holmes, King K.; Sinei, Samuel; Bwayo, Job J.; Totten, Patricia A.

doi:10.1016/s0140-6736(02)07848-0

Cited by 265 publications

(194 citation statements)

References 4 publications

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“…There is limited evidence to show that M. genitalium is associated with endometritis (Cohen et al, 2002) and pelvic inflammatory disease (Cohen et al, 2005;Short et al, 2009). M. genitalium is an extremely fastidious species that is very difficult to isolate from clinical specimens.…”

Section: Introductionmentioning

confidence: 99%

Method comparison for molecular typing of French and Tunisian Mycoplasma genitalium-positive specimens

Cazanave

Charron

Renaudin

et al. 2012

Journal of Medical Microbiology

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In this study, 76 French and Tunisian urogenital specimens were subjected to molecular typing by using the two main Mycoplasma genitalium molecular typing methods, the mgpB single nucleotide polymorphism (SNP) typing method and the combination analysis of a variable-number tandem-repeat (VNTR) marker in MG309 and mgpB SNP. Furthermore, we tried to develop a multiple-locus VNTR analysis (MLVA) method. The genome of M. genitalium G37 T was analysed for VNTRs and four VNTRs were used for an MLVA. The method, applied directly on clinical specimens, was based on a GENESCAN analysis of VNTR loci labelled with fluorescent dyes by using multiplex PCR and capillary electrophoresis. This method had a 1.00 diversity index (DI) while the mgpB SNP typing and the combination of MG309 and mgpB SNPs had DIs of 0.853 and 0.989, respectively. However, among the sets of two concurrent specimens, taken at the same time from the urogenital tracts of 12 patients, only nine had matching MLVA profiles, while the two other methods gave identical profiles for all specimens amplified, except for one set. Moreover, eight new sequence types were described with the mgpB SNP typing method. The three molecular typing methods revealed a genetic heterogeneity, suggesting that M. genitalium was endemic in France and Tunisia and that the infections were not due to the clonal dissemination of one strain. Comparison of the typing results obtained with the three methods showed that the MLVA assay seemed too discriminatory to be used in future studies of sexual networks of M. genitalium infection. According to the discriminatory power and the feasibility of each mgpB-based method, we recommend that the mgpB analysis be used for general epidemiological studies and that the combination of MG309-STR and mgpB SNP methods should be used for sexual-network studies of M. genitalium infection.

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Section: Introductionmentioning

confidence: 99%

Method comparison for molecular typing of French and Tunisian Mycoplasma genitalium-positive specimens

Cazanave

Charron

Renaudin

et al. 2012

Journal of Medical Microbiology

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“…11 Although M genitalium is now established as a relevant sexually transmitted pathogen for symptomatic men and women, little is known about the infection in the general population. Recent research has shown that M genitalium is prevalent in women with pelvic inflammatory disease 1 and acute endometritis 12 and has been shown to be able to ascend the upper genital tract 13 possibly by attaching to human spermatozoa. 14 A sero-epidemiological case-control study 15 has found an association between antibodies against M genitalium in women having tubae factor infertility when compared with women having other kinds of infertility.…”

mentioning

confidence: 99%

Mycoplasma genitalium: prevalence and behavioural risk factors in the general population

Andersen¹,

Sokolowski²,

Østergaard

et al. 2006

Sexually Transmitted Infections

126

107

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Background: Mycoplasma genitalium has been shown to cause urethritis in men and cervicitis in women and may also be a causative agent in female infertility. Objective: To estimate the prevalence of urogenital M genitalium infection and identify sexual behavioural risk factors in the general population. Methods: Participating individuals were 731 men and 921 women aged 21-23 years and not seeking the healthcare system because of symptoms. They answered questionnaires on sexual behaviour and provided samples for M genitalium testing. Results: In women aged 21-23 years, the prevalence of infection was 2.3% (21/921) and in men of the same age it was 1.1% (8/731). For both sexes, an increasing number of partners was associated with a greater chance of being infected. Among women a shorter duration of a steady relationship and having a partner with symptoms was associated with being infected, and for men younger age at first intercourse was associated with M genitalium infection. Conclusions: We conclude that the prevalence of infection in the general population is too low for populationbased screening. However, the development of test algorithms based on behavioural risk factors is a promising alternative.

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“…Universidad de Chile Walter Gübelin Harcha wgubelin@ctcinternet.cl toria pelviana (EIP) [12][13] e infertilidad 14 . Sin embargo, como ocurre con los otros micoplasma genitales, el microorganismo también ha sido detectado en personas sin signos o síntomas de infección genital 3,6 .…”

Section: Universidad De Los Andes (Wgh)unclassified

Aplicación de método molecular en la detección de Mycoplasma genitalium en hombres y en mujeres embarazadas

et al. 2006

Rev. chil. infectol.

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Association between Mycoplasma genitalium and acute endometritis

Cited by 265 publications

References 4 publications

Method comparison for molecular typing of French and Tunisian Mycoplasma genitalium-positive specimens

Method comparison for molecular typing of French and Tunisian Mycoplasma genitalium-positive specimens

Mycoplasma genitalium: prevalence and behavioural risk factors in the general population

Aplicación de método molecular en la detección de Mycoplasma genitalium en hombres y en mujeres embarazadas

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