Association between polymorphisms in HLA-A, HLA-B, HLA-DR, and DQ genes from gastric cancer and duodenal ulcer patients and cagL among cagA-positive Helicobacter pylori strains: The first study in a Turkish population

Kocak, Banu Tufan; Sarıbaş, Suat; Demiryas, Süleyman; Yılmaz, Erkan; Uysal, Ömer; Kepil, Nuray; Demırci, Mehmet; Dinç, Harika Öykü; Akkuş, Seher; Gülergün, Reyhan; Gareayaghi, Nesrin; Dagdeviren, Huseyin Emre; Özbey, Doğukan; Dağ, Hamit Harun; Tokman, Hrisi Bahar; Taşçı, İhsan

doi:10.1016/j.meegid.2020.104288

Cited by 11 publications

(11 citation statements)

References 47 publications

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“…Because H. pylori can adsorb anions and appear black, Warthin-Starry staining was used to confirm H. pylori infection. If there is H. pylori infection, there are helix rods in the gastric mucosa under Warthin-Starry staining [ 57 ]. CMTM3 and NEMO immunohistochemistry were performed as previously described, following the instructions of the antibodies [ 58 ].…”

Section: Methodsmentioning

confidence: 99%

CMTM3 protects the gastric epithelial cells from apoptosis and promotes IL-8 by stabilizing NEMO during Helicobacter pylori infection

Ning

Shi

et al. 2023

Gut Pathog

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Background CKLF-like MARVEL transmembrane domain containing 3 (CMTM3) plays an important role in cancer development. Although Helicobacter pylori (H. pylori) infection is a main cause of gastric cancer, the function of CMTM3 during H. pylori infection remains unclear. CMTM3 expression levels in tissues from H. pylori-infected patients and cells co-cultured with H. pylori were analyzed. qRT-PCR and ELISA were used to investigate the effects of CMTM3 on interleukin 8 (IL-8) expression. Annexin V/propidium iodide staining was performed to evaluate the function of CMTM3 in the apoptosis of gastric epithelial cells. Proteomic analysis was performed to explore the underlying mechanism of CMTM3 during H. pylori infection. The interaction between CMTM3 and NEMO was determined via co-immunoprecipitation, HA-ubiquitin pull-down assay, and immunofluorescence. Results H. pylori induced a significant increase in CMTM3 expression. CMTM3 inhibited gastric mucosal epithelial cells from apoptosis and increased the expression level of IL-8 during H. pylori infection. KEGG pathway enrichment analysis revealed that differentially expressed proteins were involved in epithelial cell signaling in H. pylori infection. CMTM3 directly interacted with NEMO, which promoted protein stabilization by down-regulation of its ubiquitylation. Conclusions CMTM3 reduces apoptosis and promotes IL-8 expression in the gastric epithelial cells by stabilizing NEMO during H. pylori infection. These findings characterize a new role for CMTM3 in host–pathogen interactions and provide novel insight into the molecular regulation of NEMO.

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Section: Methodsmentioning

confidence: 99%

CMTM3 protects the gastric epithelial cells from apoptosis and promotes IL-8 by stabilizing NEMO during Helicobacter pylori infection

Ning

Shi

et al. 2023

Gut Pathog

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“…A possible explanation for this association is that the binding of H. pylori to HLA-II antigens on dendritic cells in luminal and subepithelial regions could result in the transformation of naive T cells to Th1, immunosuppressive Treg and Th17 cells. Secretion of cytokines will result in strong immune responses and chronic inflammation (Kocak et al, 2020;Rezalotfi et al, 2019). The synergistic effect between some HLA genotypes and H. pylori infection may be related to some HLA genotypes that control the intensity of the immune response, inflammation as well as degrees of gastric epithelial erosions (Ohmori et al, 1997).…”

Section: H Pylori-infected Patientsmentioning

confidence: 99%

Association of human leukocyte antigen DQ-rs3920AG genotype with Helicobacter pylori recurrence in Kafrelsheikh, Egypt

Ghazy

Taha

2022

Journal of Applied Microbiology

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Aims:The aim was to evaluate the role of HLA-DP/DQ single-nucleotide polymorphisms (SNPs) in Helicobacter pylori infection in Kafrelsheikh governorate, Egypt. Methods and results:The study enrolled 120 persons; 48 naïve H. pylori-infected patients, 42 relapsers and 30 H. pylori-free controls. Gastroscopy, H. pylori stool antigen, anti-CagA and anti-VacA antibodies were determined. Genotyping of HLA-DPA1rs3077 (A/G) SNP and HLA-DQ-rs3920(A/G) SNP was done using real-time PCR. The antibody profile against H. pylori showed that 85.7% of patients with recurrent infection have IgG against CagA (p = 0.001*). There was a significant association between the occurrence of H. pylori infection and both HLA-DPA1rs3077AA and HLA-DQ-rs3920AG genotypes. Concerning H. pylori relapse, the HLA-DQ-rs3920AG genotype was detected in 78.6% of patients with recurrent infection (p = 0.002*).Patients carrying this genotype tend to be relapsers 9.8 times more than patients carrying other genotypes.Conclusions: HLA-DPA1rs3077AA and/or HLA-DQ-rs3920AG genotypes could be risk factors for the occurrence of H. pylori infection. HLA-DQ-rs3920AG genotype is markedly linked to recurrent H. pylori infection.Significance and impact of the study: Host factors as HLA gene polymorphism could be a predisposing factor for susceptibility, recurrence or chronicity of H. pylori and should be studied in different ethnic groups.

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“…In a study conducted in 2020, Saribas et al, showed that gastrointestinal diseases can be explained not only by EPIYA patterns but also by including host genetic factors such as HLAs (22). Kocak et al, reported that in patients with cagL and cagA positive H. pylori, bacterial virulence factors, as well as host genetic factors such as HLAs, are also involved in the gastrointestinal disease process (23). Like Erzin et al, (19), Sarıbaş et al, (22) and Kocak et al, (23) emphasized the importance of host factors in the process.…”

Section: In Silico Analysis Of H Pylori From Different Continentsmentioning

confidence: 99%

“…Kocak et al, reported that in patients with cagL and cagA positive H. pylori, bacterial virulence factors, as well as host genetic factors such as HLAs, are also involved in the gastrointestinal disease process (23). Like Erzin et al, (19), Sarıbaş et al, (22) and Kocak et al, (23) emphasized the importance of host factors in the process. It has strengthened our belief that our knowledge of the mechanism of cancer development of H. pylori is lacking.…”

Section: In Silico Analysis Of H Pylori From Different Continentsmentioning

confidence: 99%

In silico Analysis of Virulence, Resistance Genes and Phylogeny of Helicobacter pylori Strains from Different Continents

Demırci¹,

Ünlü²,

Kocazeybek³

2021

experimed

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Objective: Helicobacter pylori (H. pylori) is a bacterium that infects the gastric mucosa of 50% of the world population. It is known that different regional treatment practices used against the infections of H. pylori affect both the expression of virulence and antimicrobial resistance genes, giving the bacteria geographic differentiation. The aim of this study was to perform in silico analysis of virulence, resistance genes and phylogeny of H. pylori strains obtained from people living in different continents.Material and Method: Complete gene sequences of 18 H. pylori strains from six continents were downloaded from the National Center for Biotechnology Information (NCBI) database. The phylogeny of the strains, resistance and virulence genes were analyzed by CSI phylogeny, CARD and VFanalyzer, respectively.Results: African strains were the most distant identity to European strains. A2147G single nucleotide polymorphism associated with clarithromycin resistance was detected in South American and Asian origin. It was determined that strains were differentiated by a total of 95 related virulence genes under eight headings. The cagA, cagE, cagL and vacA genes were found in all strains in Asia. Conclusion:In conclusion, our study demonstrated that H. pylori strains, whose data were collected in different continents, differ from each other in terms of similarities and there is a serious difference especially in terms of virulence genes.

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Association between polymorphisms in HLA-A, HLA-B, HLA-DR, and DQ genes from gastric cancer and duodenal ulcer patients and cagL among cagA-positive Helicobacter pylori strains: The first study in a Turkish population

Cited by 11 publications

References 47 publications

CMTM3 protects the gastric epithelial cells from apoptosis and promotes IL-8 by stabilizing NEMO during Helicobacter pylori infection

CMTM3 protects the gastric epithelial cells from apoptosis and promotes IL-8 by stabilizing NEMO during Helicobacter pylori infection

Association of human leukocyte antigen DQ-rs3920AG genotype with Helicobacter pylori recurrence in Kafrelsheikh, Egypt

In silico Analysis of Virulence, Resistance Genes and Phylogeny of Helicobacter pylori Strains from Different Continents

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