Association between Polymorphisms of Hemochromatosis (HFE), Blood Lead (Pb) Levels, and DNA Oxidative Damage in Battery Workers

Gomes, Willian Robert; Devóz, Paula Pícoli; Rocha, Bruno Alves; Grotto, Denise; Serpeloni, Juliana Mara; Batista, Bruno Lemos; Asimakopoulos, Alexandros G.; Kannan, Kurunthachalam; Barbosa, Fernando; Barcelos, Gustavo Rafael Mazzaron

doi:10.3390/ijerph20043513

Cited by 3 publications

(1 citation statement)

References 50 publications

(57 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…However, a study accomplished on 36 Spanish patients C282Y and H63D mutations showed no differences between patients healthy people [33]. Another study mentioned to the relation between HFE polymorphisms and DNA damage by oxidative agents [34]. Further, a study found that C282Y mutation causes overload of serum ferritin level at 482 μg/L in female [35].…”

Section: Resultsmentioning

confidence: 99%

HFE Gene Mutations as Predisposing Factors for Childhood Acute Lymphoblastic Leukaemia in Iraqi Patients

Ibrahim,

Albandar,

Jabbar

et al. 2024

UTJsci

View full text Add to dashboard Cite

Hemochromatosis is a prevalent hereditary disorder that causes excess iron to build up in the body to dangerous levels. Hereditary hemochromatosis, also known as HFE-related hemochromatosis is carried on by changes in the HFE gene. Investigating the gene mutations of the HFE gene is a way to explore the prevalence of this disease. This study aims to determine the association between hemochromatosis HFE gene mutations (C282Y and H63D) and childhood acute lymphoblastic leukaemia in patients at Basra Specialized Hospital for Children and AL-sadder Teaching Hospital in the Basra governance. QIAamp DNA and Blood Mini Kit were used to isolate and identify Human genomic DNA and detect mutations in the HFE gene using the DNA hybridization method. In this study, the absence of the C282Y mutations in both patients and the control group was identified. However, testing DNA-based hybridization experiments revealed low detection levels of the H63D (homozygous, heterozygous) mutations; in only 12.5% of patients. The H63D (only homozygous) mutations were present in 10% of the control group. The association between patients and the control group is considered statistically significant. The HFE gene mutations (C282Y and H63D), originate in acute lymphoblastic leukaemia in childhood, thus, this study recommends complementary investigations to illustrate this case in more detail with more cases of patients and discover the hidden agents underlying these mutations.

show abstract

Section: Resultsmentioning

confidence: 99%

HFE Gene Mutations as Predisposing Factors for Childhood Acute Lymphoblastic Leukaemia in Iraqi Patients

Ibrahim,

Albandar,

Jabbar

et al. 2024

UTJsci

View full text Add to dashboard Cite

show abstract

Combating Lead (Pb) Contamination: Integrating Biomonitoring, Advanced Detection, and Remediation for Environmental and Public Health

Zafar,

Fatima,

Asad

et al. 2024

Water Air Soil Pollut

View full text Add to dashboard Cite

Environmentally Relevant Lead Exposure Impacts Gene Expression in SH-SY5Y Cells Throughout Neuronal Differentiation

Morgan,

Tapaswi,

Polemi

et al. 2024

Preprint

View full text Add to dashboard Cite

Lead (Pb) causes learning and memory impairments, but the molecular effects of continuous, environmentally relevant levels of exposure on key neurodevelopmental processes are not fully characterized. Here we examine the effects of a range of environmentally relevant Pb concentrations (0.16μM, 1.26μM, and 10μM Pb) relative to control on neural differentiation in the SH-SY5Y cell model. Pb exposure began on differentiation day 5 and was continuous for remaining days, after which we assessed the transcriptome via RNA sequencing at several time points. The bulk of detected changes in gene expression occurred with the 10μM Pb condition. Interestingly, changes associated with the lower two exposures were differentiation stage-specific, with aberrant expression of several genes (e.g., COL3A1, HMOX1, and CCL2) observed during differentiation on days 9, 12, and 15 in both the 0.16μM and 1.26μM Pb conditions, and which disappeared by the time differentiation concluded on day 18. We observed six co-expression clusters of genes during differentiation, and 10μM Pb significantly perturbed two clusters, one involved in cell cycling and DNA repair and the other in protein synthesis. Benchmark concentration analysis identified many genes affected by levels of Pb at or below the current US standard (3.5μg/dL) and these genes were enriched for pathways including stress responses, DNA repair, misfolded protein response, mitosis, and neurotransmitter production. This work highlights potential new mechanisms by which environmentally relevant concentrations of Pb impact gene expression throughout neural differentiation and result in long-lasting implications for neural health and cognition.

show abstract

Association between Polymorphisms of Hemochromatosis (HFE), Blood Lead (Pb) Levels, and DNA Oxidative Damage in Battery Workers

Cited by 3 publications

References 50 publications

HFE Gene Mutations as Predisposing Factors for Childhood Acute Lymphoblastic Leukaemia in Iraqi Patients

HFE Gene Mutations as Predisposing Factors for Childhood Acute Lymphoblastic Leukaemia in Iraqi Patients

Combating Lead (Pb) Contamination: Integrating Biomonitoring, Advanced Detection, and Remediation for Environmental and Public Health

Environmentally Relevant Lead Exposure Impacts Gene Expression in SH-SY5Y Cells Throughout Neuronal Differentiation

Contact Info

Product

Resources

About