Association between polymorphisms of Myf5 and POU1F1 genes with growth and carcass traits in Hanwoo (Korean cattle)

Abstract

Myogenic factor 5 (Myf5) and POU class 1 homeobox 1 (POU1F1) genes play important roles in growth and development of mammals. Bovine Myf5 and POU1F1 were characterized to detect genetic variation at these loci and to replace them to economic traits in 367 cattle representing Hanwoo (325) and Angus (37). Two single nucleotide polymorphisms (SNPs) were identified in intron 2 (A1948G SNP) of Myf5 and exon 6 (A15635G SNP) of POU1F1 by sequence analyses of genomic DNA. Statistical analysis indicated that the Myf5 polymorphisms significantly (0.05) associated backfat thickness and live weight at 6-months-of-age and that POU1F1 polymorphisms significantly influenced carcass weight and live weight at 24-month of age, and backfat thickness. The interaction between Myf5 and POU1F1 was significant on carcass weight, M. longissimus dorsi area, backfat thickness and marbling score. The results implicate Myf5 and POU1F1 as candidate genes of growth and carcass traits, and suggest that the interaction between Myf5 and POU1F1 strongly affect growth and carcass traits in cattle.

Identification of a Single Nucleotide Polymorphism at Hinf-1 Enzyme Restriction Site of Pit-1 Gene on Indonesian Bali Cattle Population

“…The same mutation (GàA) was also found, and classified as silent mutation, in exon 6 of Pit-1 gene (Seong et al, 2011) which was speculated to have no direct effect on phenotype variation . However, the relation between Pit-1|Hinf-1 polymorphism and carcass quality are significant for Hanwoo cattle (Seong et al, 2011;Han et al, 2010) as well as the relationship between the polymorphism and intramuscular fat, in Brangus bulls (Thomas et al, 2007) or production traits, in Qinchuan cattle (Zhang et al, 2009). …”

“…Besides, the study of this gene was also intensively conducted in native cattle breeds in some countries, including Iranian cattle (Javanmard et al, 2005), Southern Anatolian red cattle (Oztabak et al, 2008), Hanwoo cattle (Han et al, 2010;Seong et al, 2011), Turkey cattle (Ozdemir, 2012), Qinchuan cattle (Zhang et al, 2009) and Piemontese cattle (Ribeca et al, 2014). Even more, Pit-1 gene was also identified in non-cattle species, including chicken (Nie et al, 2008), sheep (Bastos et al, 2006) and buffalo (Javanmard et al, 2005;Misrianti et al, 2010).…”

“…Restriction fragment length polymorphism (RFLP) provided first evidence of high polymorphism in this gene at Hinf-1 restriction sites, located at 5 and exon 6 (Woolard et al, 1994). The polymorphism, so-called Pit-1|Hinf-1 polymorphism, is indicated by base substitution at the restriction site, in which G is changed to A (Seong et al, 2011). Yet, the change has no effect on the primary structure of transcripted polypeptide (silent mutation).…”

“…It is interesting that in beef cattle, Pit-1|Hinf-1 polymorphism significantly affects growth rate of Podolica cattle (Salvagi et al, 2011), Hanwoo cattle (Seong et al, 2011) and Nanyang cattle (Kai et al, 2006). These evidences promoted Pit-1|Hinf-1 polymorphism as a promising gene marker for MAS.…”

Association between polymorphisms of the CRH and POMC genes with economic traits in Korean cattle (Hanwoo)

“…The native cattle in Korea is considered to belong to European cattle breeds (Mannen et al, 1998(Mannen et al, , 2004Seong et al, 2011). Hanwoo (Korean native cattle) refers to a breed of cattle raised in Korea (Bos taurus coreanae) and Hanwoo is regarded as a premium beef because of its high palatability and desired chewiness.…”