Association Between PON1 L55M Polymorphism and PON1 Enzyme Activity in Patients with Leukemia.

Abstract

Paraoxonase 1 (PON1) is an important antioxidant enzyme which has a role in preventing the effects of systemic oxidative stress. The purpose of our study was to investigate the possible association between PON1 L55M polymorphism and leukemia development and to determine the relationship between PON1 genotypes and PON1 enzyme activities. Genotypes of 102 cases and 112 healthy controls were determined by PCR-RFLP. PON1 enzyme activity was determined according to Eckerson's method. The ratio of MM genotype belonging to PON1 L55M polymorphism in control group was 6.3% and was 7.8% in patients with leukemia (p= 0.39). PON1 enzyme activity was 118.8 ± 115.1 U/mL in control group, while decreased to 75.6 ± 64.4 U/mL in patients with leukemia (p= 0.004). PON1 enzyme activities of the individuals with MM genotypes belonging to PON1 L55M polymorphism was 57.43 ± 21.61 U/ mL in control group and decreased to 39.18 ± 45.61 U/mL in leukemic patients. Our results suggest that, PON1 L55M polymorphism genotype ratios do not affect leukemia development. However, reduced PON1 enzyme activity and also the combination of PON1 L55M polymorphism with reduced PON1 enzyme activity are associated with the increased risk of leukemia. Furthermore, older age may be a risk factor for developing leukemia. PON1 enzim aktivitesi Eckerson yöntemi ile ölçüldü. PON1 L55M polimorfizmine ait MM genotip oranları kontrol grubunda %6.3 iken lösemili hastalarda % 7.8 idi (p= 0.39). PON1 enzim aktivitesi kontrol grubunda 118.8 ± 115.1 U/mL iken lösemili hastalarda düşük olarak saptandı (75.6 ± 64.4 U/mL, p= 0.004). PON1 L55M polimorfizmine ait MM genotipli bireylerin PON1 enzim aktivitesi kontrol grubunda 57.43 ± 21.61 U/mL iken lösemili hastalarda düşük olarak saptandı (39.18 ± 45.61 U/mL). Bizim bulgularımız PON1 L55M polimorfizmi genotip oranlarının lösemi gelişimini etkilemediğini göstermektedir. Bununla birlikte, düşük PON1 enzim aktivitesi tek başına ve PON1 L55M polimorfizmi ile birlikte lösemi riski artışı ile ilişkilidir. Ayrıca ileri yaş lösemiye yakalanma olasılığını yükseltir.

An investigation of the relation between catalase C262T gene polymorphism and catalase enzyme activity in leukemia patients

“…These individuals participated in a previously published case-control study. The cases and controls have been described previously in terms of data collection and study procedures [26]. This study has the approval of the Ethics Committee, Faculty of Medicine, The University of Mersin.…”

Clinical significance of PON1 L55M, Q192R and I102V polymorphisms and their association with prostate cancer risk in Polish men

“…Variations due to the presence of single nucleotide polymorphisms in antioxidant enzyme genes may contribute to the interindividual differences of transcript levels and enzyme activities, which might play a significant role in the prostate cancer development [14,22]. Therefore, the aim of the present study was to investigate the relation between PON1 L55M, Q192R and I102V polymorphisms and prostate can-cer risk in Polish men.…”

“…It was suggested that PON1 55M allele could correlate with higher PON1 activity than PON1 55L allele. In another study, it was observed that the differences in the PON1 serum levels linked to the L55M polymorphism were due to the reduced stability of the PON1 55M protein [22]. The hydrolyzing activity of the protein encoded by the 192RR, second the most common genotype of PON1 gene, is 8-fold higher than that encoded by the 192QQ.…”