Association between pregnancy-associatedα 2-glycoprotein (α 2-PAG) and mixed leucocyte reaction determinants on the leucocyte surface

Horne, C. H. W.; Thomson, A. W.; Hunter, C. B. J.; Heyningen, Veronica van; Deane, David; Steel, C. M.

doi:10.1007/bf01964383

Cited by 15 publications

(2 citation statements)

References 22 publications

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“…We have noted on SDS-PAGE gels run with labelled glycoprotein preparations from EB1, that there is a deficiency of glycoprotein of MW 28,000. Horne et al (1979) have shown that the "anti-LAD'' serum strongly inhibits the binding to peripheral lymphocytes of fluorescein-labelled purified IgG against pregnancy-associated a,-glycoprotein, and we have recently demonstrated (unpublished data) the blocking of Fc receptors on B-LCL by "anti-LAD'' serum. The significance of these associations remains to be further assessed.…”

Section: Discussionmentioning

confidence: 75%

Serological Distinction between DR Antigens and Lymphocyte Activating Determinants

et al. 1981

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The Burkitt lymphoma (BL)‐derived, HLA‐DR antigen positive B cell line, EB1, is a consistently low stimulator in MLC. A rabbit antiserum raised against the strongly stimulating BL line DAUDI, after appropriate absorption with EB1, inhibits MLC stimulation by both B cell lines and allogeneic lymphocytes, whilst lectin‐induced proliferation is not significantly affected. Indirect immunofluorescence and 125I‐staphylococcal protein A binding to cells pre‐incubated with this antiserum suggest that the antigen is present on both peripheral B and T cells, as well as on B lymphoblastoid and myeloma lines. We suggest that this antiserum is directed against lymphocyte activating determinant(s) (LADs) and that these are distinct from the serologically defined DR antigens.

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Section: Discussionmentioning

confidence: 75%

Serological Distinction between DR Antigens and Lymphocyte Activating Determinants

et al. 1981

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“…Some investigators have found that the in vitro response to phytohaemagglutinin (PHA) is depressed in lymphocytes from pregnant women (Purtilo et al, 1972;Leiken, 1972) while others have found that as long as the culture system was free of maternal serum the response was normal (Daunter et al, 1979). The depressing effect of maternal serum on the lymphocyte response to lectins has been attributed to an increase in the a2-glycoprotein (Horne et al, 1979), increase in serum alphafetoprotein (Figueredo et al, 1979) or to steroid or other hormones (Kasakura, 1973;Morse et al, 1976). Some investigators have found that at very high concentrations the steroid hormones associated with pregnancy cause some inhibition of the lymphocyte response to PHA (Ablin et al, 1974;Mendelsohn et al, 1977) but others (Schiff et al, 1975) considered the concentrations necessary for inhibition were such that the steroid hormones tested would not have a significant effect in pregnancy.…”

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confidence: 99%

The Effect of Progesterone, Oestrogens and Hydrocortisone on the Mitogenic Response of Lymphocytes to Phytohaemagglutinin in Pregnant and Non‐pregnant Women

Skinnider

Laxdal

1981

BJOG

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Summary The mitogenic response to phytohaemagglutinin of lymphocytes from women in the third trimester of pregnancy is not inhibited by progesterone, oestriol or oestradiol even at much greater than normal physiological levels. There was no difference in the response of lymphocytes from pregnant or non‐pregnant women. Inhibition in the mitogenic response to phytohaemagglutinin was achieved with hydrocortisone but not at the physiological levels found in pregnancy. The results from this in vitro study would not support the view that the level of steroid hormones in pregnancy significantly affects the immunological status of the mothers.

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Immunohistochemical definition of antigenic determinants of pregnancy‐associated α₂‐glycoprotein (α₂‐PAG) using monoclonal antibodies

Smith

Horne

Carpenter

et al. 1988

The Journal of Pathology

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Human pregnancy-associated alpha 2-glycoprotein (alpha 2-PAG) is a high molecular weight glycoprotein in normal sera. The protein is present in high concentration in the sera of pregnant females and in abnormally low concentration in association with conditions connected with abnormalities of mucosal immunity. Indirect immunoperoxidase techniques using poly- and monoclonal antibodies were employed to identify pregnancy-associated alpha 2-PAG in different tissues. Four monoclonal antibodies were selected from a battery of antibodies with defined specificities in order to ascertain reactivity with various epitopes of the antigen. The antibodies were applied to paraffin sections of breast, colon, salivary gland, and tonsil, and different fixation regimes were used in the preparation of the tissues. The polyclonal antibodies were found to stain plasma cells and epithelial lumina evenly in all the tissues included whereas the monoclonal antibodies were shown to stain certain components selectively. In breast and salivary glands, all four monoclonal antibodies could identify alpha 2-PAG, but in tonsil and colon, only two were reactive. This difference in epitope expression might reflect the internal processing of alpha 2-PAG, and lack of availability of certain epitopes may be indicative of functional blocking of certain domains.

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Association between pregnancy-associatedα 2-glycoprotein (α 2-PAG) and mixed leucocyte reaction determinants on the leucocyte surface

Cited by 15 publications

References 22 publications

Serological Distinction between DR Antigens and Lymphocyte Activating Determinants

Serological Distinction between DR Antigens and Lymphocyte Activating Determinants

The Effect of Progesterone, Oestrogens and Hydrocortisone on the Mitogenic Response of Lymphocytes to Phytohaemagglutinin in Pregnant and Non‐pregnant Women

Immunohistochemical definition of antigenic determinants of pregnancy‐associated α₂‐glycoprotein (α₂‐PAG) using monoclonal antibodies

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Association between pregnancy-associatedα 2-glycoprotein (α 2-PAG) and mixed leucocyte reaction determinants on the leucocyte surface

Cited by 15 publications

References 22 publications

Serological Distinction between DR Antigens and Lymphocyte Activating Determinants

Serological Distinction between DR Antigens and Lymphocyte Activating Determinants

The Effect of Progesterone, Oestrogens and Hydrocortisone on the Mitogenic Response of Lymphocytes to Phytohaemagglutinin in Pregnant and Non‐pregnant Women

Immunohistochemical definition of antigenic determinants of pregnancy‐associated α2‐glycoprotein (α2‐PAG) using monoclonal antibodies

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Immunohistochemical definition of antigenic determinants of pregnancy‐associated α₂‐glycoprotein (α₂‐PAG) using monoclonal antibodies